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Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis

Myosin VI plays a role in the maintenance of Golgi morphology and in exocytosis. In a yeast 2-hybrid screen we identified optineurin as a binding partner for myosin VI at the Golgi complex and confirmed this interaction in a range of protein interaction studies. Both proteins colocalize at the Golgi...

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Autores principales: Sahlender, Daniela A., Roberts, Rhys C., Arden, Susan D., Spudich, Giulietta, Taylor, Marcus J., Luzio, J. Paul, Kendrick-Jones, John, Buss, Folma
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171882/
https://www.ncbi.nlm.nih.gov/pubmed/15837803
http://dx.doi.org/10.1083/jcb.200501162
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author Sahlender, Daniela A.
Roberts, Rhys C.
Arden, Susan D.
Spudich, Giulietta
Taylor, Marcus J.
Luzio, J. Paul
Kendrick-Jones, John
Buss, Folma
author_facet Sahlender, Daniela A.
Roberts, Rhys C.
Arden, Susan D.
Spudich, Giulietta
Taylor, Marcus J.
Luzio, J. Paul
Kendrick-Jones, John
Buss, Folma
author_sort Sahlender, Daniela A.
collection PubMed
description Myosin VI plays a role in the maintenance of Golgi morphology and in exocytosis. In a yeast 2-hybrid screen we identified optineurin as a binding partner for myosin VI at the Golgi complex and confirmed this interaction in a range of protein interaction studies. Both proteins colocalize at the Golgi complex and in vesicles at the plasma membrane. When optineurin is depleted from cells using RNA interference, myosin VI is lost from the Golgi complex, the Golgi is fragmented and exocytosis of vesicular stomatitis virus G-protein to the plasma membrane is dramatically reduced. Two further binding partners for optineurin have been identified: huntingtin and Rab8. We show that myosin VI and Rab8 colocalize around the Golgi complex and in vesicles at the plasma membrane and overexpression of constitutively active Rab8-Q67L recruits myosin VI onto Rab8-positive structures. These results show that optineurin links myosin VI to the Golgi complex and plays a central role in Golgi ribbon formation and exocytosis.
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spelling pubmed-21718822008-03-05 Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis Sahlender, Daniela A. Roberts, Rhys C. Arden, Susan D. Spudich, Giulietta Taylor, Marcus J. Luzio, J. Paul Kendrick-Jones, John Buss, Folma J Cell Biol Research Articles Myosin VI plays a role in the maintenance of Golgi morphology and in exocytosis. In a yeast 2-hybrid screen we identified optineurin as a binding partner for myosin VI at the Golgi complex and confirmed this interaction in a range of protein interaction studies. Both proteins colocalize at the Golgi complex and in vesicles at the plasma membrane. When optineurin is depleted from cells using RNA interference, myosin VI is lost from the Golgi complex, the Golgi is fragmented and exocytosis of vesicular stomatitis virus G-protein to the plasma membrane is dramatically reduced. Two further binding partners for optineurin have been identified: huntingtin and Rab8. We show that myosin VI and Rab8 colocalize around the Golgi complex and in vesicles at the plasma membrane and overexpression of constitutively active Rab8-Q67L recruits myosin VI onto Rab8-positive structures. These results show that optineurin links myosin VI to the Golgi complex and plays a central role in Golgi ribbon formation and exocytosis. The Rockefeller University Press 2005-04-25 /pmc/articles/PMC2171882/ /pubmed/15837803 http://dx.doi.org/10.1083/jcb.200501162 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Sahlender, Daniela A.
Roberts, Rhys C.
Arden, Susan D.
Spudich, Giulietta
Taylor, Marcus J.
Luzio, J. Paul
Kendrick-Jones, John
Buss, Folma
Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis
title Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis
title_full Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis
title_fullStr Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis
title_full_unstemmed Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis
title_short Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis
title_sort optineurin links myosin vi to the golgi complex and is involved in golgi organization and exocytosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171882/
https://www.ncbi.nlm.nih.gov/pubmed/15837803
http://dx.doi.org/10.1083/jcb.200501162
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