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Molecular constituents of neuronal AMPA receptors

Dynamic regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) underlies aspects of synaptic plasticity. Although numerous AMPAR-interacting proteins have been identified, their quantitative and relative contributions to native AMPAR complexes remain unclear. Here, we...

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Autores principales: Fukata, Yuko, Tzingounis, Anastassios V., Trinidad, Jonathan C., Fukata, Masaki, Burlingame, Alma L., Nicoll, Roger A., Bredt, David S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171936/
https://www.ncbi.nlm.nih.gov/pubmed/15883194
http://dx.doi.org/10.1083/jcb.200501121
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author Fukata, Yuko
Tzingounis, Anastassios V.
Trinidad, Jonathan C.
Fukata, Masaki
Burlingame, Alma L.
Nicoll, Roger A.
Bredt, David S.
author_facet Fukata, Yuko
Tzingounis, Anastassios V.
Trinidad, Jonathan C.
Fukata, Masaki
Burlingame, Alma L.
Nicoll, Roger A.
Bredt, David S.
author_sort Fukata, Yuko
collection PubMed
description Dynamic regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) underlies aspects of synaptic plasticity. Although numerous AMPAR-interacting proteins have been identified, their quantitative and relative contributions to native AMPAR complexes remain unclear. Here, we quantitated protein interactions with neuronal AMPARs by immunoprecipitation from brain extracts. We found that stargazin-like transmembrane AMPAR regulatory proteins (TARPs) copurified with neuronal AMPARs, but we found negligible binding to GRIP, PICK1, NSF, or SAP-97. To facilitate purification of neuronal AMPAR complexes, we generated a transgenic mouse expressing an epitope-tagged GluR2 subunit of AMPARs. Taking advantage of this powerful new tool, we isolated two populations of GluR2 containing AMPARs: an immature complex with the endoplasmic reticulum chaperone immunoglobulin-binding protein and a mature complex containing GluR1, TARPs, and PSD-95. These studies establish TARPs as the auxiliary components of neuronal AMPARs.
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spelling pubmed-21719362008-03-05 Molecular constituents of neuronal AMPA receptors Fukata, Yuko Tzingounis, Anastassios V. Trinidad, Jonathan C. Fukata, Masaki Burlingame, Alma L. Nicoll, Roger A. Bredt, David S. J Cell Biol Research Articles Dynamic regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) underlies aspects of synaptic plasticity. Although numerous AMPAR-interacting proteins have been identified, their quantitative and relative contributions to native AMPAR complexes remain unclear. Here, we quantitated protein interactions with neuronal AMPARs by immunoprecipitation from brain extracts. We found that stargazin-like transmembrane AMPAR regulatory proteins (TARPs) copurified with neuronal AMPARs, but we found negligible binding to GRIP, PICK1, NSF, or SAP-97. To facilitate purification of neuronal AMPAR complexes, we generated a transgenic mouse expressing an epitope-tagged GluR2 subunit of AMPARs. Taking advantage of this powerful new tool, we isolated two populations of GluR2 containing AMPARs: an immature complex with the endoplasmic reticulum chaperone immunoglobulin-binding protein and a mature complex containing GluR1, TARPs, and PSD-95. These studies establish TARPs as the auxiliary components of neuronal AMPARs. The Rockefeller University Press 2005-05-09 /pmc/articles/PMC2171936/ /pubmed/15883194 http://dx.doi.org/10.1083/jcb.200501121 Text en Copyright © 2005, The Rockefeller University Press https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/ (https://creativecommons.org/licenses/by-nc-sa/4.0/) ).
spellingShingle Research Articles
Fukata, Yuko
Tzingounis, Anastassios V.
Trinidad, Jonathan C.
Fukata, Masaki
Burlingame, Alma L.
Nicoll, Roger A.
Bredt, David S.
Molecular constituents of neuronal AMPA receptors
title Molecular constituents of neuronal AMPA receptors
title_full Molecular constituents of neuronal AMPA receptors
title_fullStr Molecular constituents of neuronal AMPA receptors
title_full_unstemmed Molecular constituents of neuronal AMPA receptors
title_short Molecular constituents of neuronal AMPA receptors
title_sort molecular constituents of neuronal ampa receptors
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171936/
https://www.ncbi.nlm.nih.gov/pubmed/15883194
http://dx.doi.org/10.1083/jcb.200501121
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