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Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis

In contrast to its well-established actions as an organizer of synaptic differentiation at the neuromuscular junction, the proteoglycan agrin is still in search of a function in the nervous system. Here, we report an entirely unanticipated role for agrin in the dual modulation of electrical and chem...

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Detalles Bibliográficos
Autores principales: Martin, Agnès O., Alonso, Gérard, Guérineau, Nathalie C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171940/
https://www.ncbi.nlm.nih.gov/pubmed/15883200
http://dx.doi.org/10.1083/jcb.200411054
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author Martin, Agnès O.
Alonso, Gérard
Guérineau, Nathalie C.
author_facet Martin, Agnès O.
Alonso, Gérard
Guérineau, Nathalie C.
author_sort Martin, Agnès O.
collection PubMed
description In contrast to its well-established actions as an organizer of synaptic differentiation at the neuromuscular junction, the proteoglycan agrin is still in search of a function in the nervous system. Here, we report an entirely unanticipated role for agrin in the dual modulation of electrical and chemical intercellular communication that occurs during the critical period of synapse formation. When applied at the developing splanchnic nerve–chromaffin cell cholinergic synapse in rat adrenal acute slices, agrin rapidly modified cell-to-cell communication mechanisms. Specifically, it led to decreased gap junction–mediated electrical coupling that preceded an increase in nicotinic synaptic transmission. This developmental switch from predominantly electrical to chemical communication was fully operational within one hour and depended on the activation of Src family–related tyrosine kinases. Hence, agrin may play a pivotal role in synaptogenesis in promoting a rapid switch between electrical coupling and synaptic neurotransmission.
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spelling pubmed-21719402008-03-05 Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis Martin, Agnès O. Alonso, Gérard Guérineau, Nathalie C. J Cell Biol Research Articles In contrast to its well-established actions as an organizer of synaptic differentiation at the neuromuscular junction, the proteoglycan agrin is still in search of a function in the nervous system. Here, we report an entirely unanticipated role for agrin in the dual modulation of electrical and chemical intercellular communication that occurs during the critical period of synapse formation. When applied at the developing splanchnic nerve–chromaffin cell cholinergic synapse in rat adrenal acute slices, agrin rapidly modified cell-to-cell communication mechanisms. Specifically, it led to decreased gap junction–mediated electrical coupling that preceded an increase in nicotinic synaptic transmission. This developmental switch from predominantly electrical to chemical communication was fully operational within one hour and depended on the activation of Src family–related tyrosine kinases. Hence, agrin may play a pivotal role in synaptogenesis in promoting a rapid switch between electrical coupling and synaptic neurotransmission. The Rockefeller University Press 2005-05-09 /pmc/articles/PMC2171940/ /pubmed/15883200 http://dx.doi.org/10.1083/jcb.200411054 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Martin, Agnès O.
Alonso, Gérard
Guérineau, Nathalie C.
Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis
title Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis
title_full Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis
title_fullStr Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis
title_full_unstemmed Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis
title_short Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis
title_sort agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171940/
https://www.ncbi.nlm.nih.gov/pubmed/15883200
http://dx.doi.org/10.1083/jcb.200411054
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