MCAK associates with the tips of polymerizing microtubules

MCAK is a member of the kinesin-13 family of microtubule (MT)-depolymerizing kinesins. We show that the potent MT depolymerizer MCAK tracks (treadmills) with the tips of polymerizing MTs in living cells. Tip tracking of MCAK is inhibited by phosphorylation and is dependent on the extreme COOH-termin...

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Detalles Bibliográficos
Autores principales: Moore, Ayana T., Rankin, Kathleen E., von Dassow, George, Peris, Leticia, Wagenbach, Michael, Ovechkina, Yulia, Andrieux, Annie, Job, Didier, Wordeman, Linda
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171944/
https://www.ncbi.nlm.nih.gov/pubmed/15883193
http://dx.doi.org/10.1083/jcb.200411089
Descripción
Sumario:MCAK is a member of the kinesin-13 family of microtubule (MT)-depolymerizing kinesins. We show that the potent MT depolymerizer MCAK tracks (treadmills) with the tips of polymerizing MTs in living cells. Tip tracking of MCAK is inhibited by phosphorylation and is dependent on the extreme COOH-terminal tail of MCAK. Tip tracking is not essential for MCAK's MT-depolymerizing activity. We propose that tip tracking is a mechanism by which MCAK is preferentially localized to regions of the cell that modulate the plus ends of MTs.

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