MCAK associates with the tips of polymerizing microtubules

MCAK is a member of the kinesin-13 family of microtubule (MT)-depolymerizing kinesins. We show that the potent MT depolymerizer MCAK tracks (treadmills) with the tips of polymerizing MTs in living cells. Tip tracking of MCAK is inhibited by phosphorylation and is dependent on the extreme COOH-termin...

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Autores principales: Moore, Ayana T., Rankin, Kathleen E., von Dassow, George, Peris, Leticia, Wagenbach, Michael, Ovechkina, Yulia, Andrieux, Annie, Job, Didier, Wordeman, Linda
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171944/
https://www.ncbi.nlm.nih.gov/pubmed/15883193
http://dx.doi.org/10.1083/jcb.200411089
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author Moore, Ayana T.
Rankin, Kathleen E.
von Dassow, George
Peris, Leticia
Wagenbach, Michael
Ovechkina, Yulia
Andrieux, Annie
Job, Didier
Wordeman, Linda
author_facet Moore, Ayana T.
Rankin, Kathleen E.
von Dassow, George
Peris, Leticia
Wagenbach, Michael
Ovechkina, Yulia
Andrieux, Annie
Job, Didier
Wordeman, Linda
author_sort Moore, Ayana T.
collection PubMed
description MCAK is a member of the kinesin-13 family of microtubule (MT)-depolymerizing kinesins. We show that the potent MT depolymerizer MCAK tracks (treadmills) with the tips of polymerizing MTs in living cells. Tip tracking of MCAK is inhibited by phosphorylation and is dependent on the extreme COOH-terminal tail of MCAK. Tip tracking is not essential for MCAK's MT-depolymerizing activity. We propose that tip tracking is a mechanism by which MCAK is preferentially localized to regions of the cell that modulate the plus ends of MTs.
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spelling pubmed-21719442008-03-05 MCAK associates with the tips of polymerizing microtubules Moore, Ayana T. Rankin, Kathleen E. von Dassow, George Peris, Leticia Wagenbach, Michael Ovechkina, Yulia Andrieux, Annie Job, Didier Wordeman, Linda J Cell Biol Research Articles MCAK is a member of the kinesin-13 family of microtubule (MT)-depolymerizing kinesins. We show that the potent MT depolymerizer MCAK tracks (treadmills) with the tips of polymerizing MTs in living cells. Tip tracking of MCAK is inhibited by phosphorylation and is dependent on the extreme COOH-terminal tail of MCAK. Tip tracking is not essential for MCAK's MT-depolymerizing activity. We propose that tip tracking is a mechanism by which MCAK is preferentially localized to regions of the cell that modulate the plus ends of MTs. The Rockefeller University Press 2005-05-09 /pmc/articles/PMC2171944/ /pubmed/15883193 http://dx.doi.org/10.1083/jcb.200411089 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Moore, Ayana T.
Rankin, Kathleen E.
von Dassow, George
Peris, Leticia
Wagenbach, Michael
Ovechkina, Yulia
Andrieux, Annie
Job, Didier
Wordeman, Linda
MCAK associates with the tips of polymerizing microtubules
title MCAK associates with the tips of polymerizing microtubules
title_full MCAK associates with the tips of polymerizing microtubules
title_fullStr MCAK associates with the tips of polymerizing microtubules
title_full_unstemmed MCAK associates with the tips of polymerizing microtubules
title_short MCAK associates with the tips of polymerizing microtubules
title_sort mcak associates with the tips of polymerizing microtubules
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171944/
https://www.ncbi.nlm.nih.gov/pubmed/15883193
http://dx.doi.org/10.1083/jcb.200411089
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