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Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis

During heart morphogenesis, cardiac chambers arise by differential expansion of regions of the primitive cardiac tube. This process is under the control of specific transcription factors such as Tbx5 and dHAND. To gain insight into the cellular mechanisms that underlie cardiogenesis, we have used a...

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Autores principales: Meilhac, Sigolène M., Esner, Milan, Kerszberg, Michel, Moss, Julie E., Buckingham, Margaret E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171975/
https://www.ncbi.nlm.nih.gov/pubmed/14709543
http://dx.doi.org/10.1083/jcb.200309160
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author Meilhac, Sigolène M.
Esner, Milan
Kerszberg, Michel
Moss, Julie E.
Buckingham, Margaret E.
author_facet Meilhac, Sigolène M.
Esner, Milan
Kerszberg, Michel
Moss, Julie E.
Buckingham, Margaret E.
author_sort Meilhac, Sigolène M.
collection PubMed
description During heart morphogenesis, cardiac chambers arise by differential expansion of regions of the primitive cardiac tube. This process is under the control of specific transcription factors such as Tbx5 and dHAND. To gain insight into the cellular mechanisms that underlie cardiogenesis, we have used a retrospective clonal approach based on the spontaneous recombination of an nlaacZ reporter gene targeted to the murine α-cardiac actin locus. We show that clonal growth of myocardial cells is oriented. At embryonic day (E) 10.5, the shape of clones is characteristic of a given cardiac region and reflects its morphology. This is already detectable in the primitive cardiac tube at E8.5, and is maintained after septation at E14.5 with additional modulations. The clonal analysis reveals new subdivisions of the myocardium, including an interventricular boundary region. Our results show that the myocardium, from the time of its formation, is a polarized and regionalized tissue and point to the role of oriented clonal cell growth in cardiac chamber morphogenesis.
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spelling pubmed-21719752008-03-05 Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis Meilhac, Sigolène M. Esner, Milan Kerszberg, Michel Moss, Julie E. Buckingham, Margaret E. J Cell Biol Article During heart morphogenesis, cardiac chambers arise by differential expansion of regions of the primitive cardiac tube. This process is under the control of specific transcription factors such as Tbx5 and dHAND. To gain insight into the cellular mechanisms that underlie cardiogenesis, we have used a retrospective clonal approach based on the spontaneous recombination of an nlaacZ reporter gene targeted to the murine α-cardiac actin locus. We show that clonal growth of myocardial cells is oriented. At embryonic day (E) 10.5, the shape of clones is characteristic of a given cardiac region and reflects its morphology. This is already detectable in the primitive cardiac tube at E8.5, and is maintained after septation at E14.5 with additional modulations. The clonal analysis reveals new subdivisions of the myocardium, including an interventricular boundary region. Our results show that the myocardium, from the time of its formation, is a polarized and regionalized tissue and point to the role of oriented clonal cell growth in cardiac chamber morphogenesis. The Rockefeller University Press 2004-01-05 /pmc/articles/PMC2171975/ /pubmed/14709543 http://dx.doi.org/10.1083/jcb.200309160 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Meilhac, Sigolène M.
Esner, Milan
Kerszberg, Michel
Moss, Julie E.
Buckingham, Margaret E.
Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis
title Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis
title_full Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis
title_fullStr Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis
title_full_unstemmed Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis
title_short Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis
title_sort oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171975/
https://www.ncbi.nlm.nih.gov/pubmed/14709543
http://dx.doi.org/10.1083/jcb.200309160
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