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Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis
During heart morphogenesis, cardiac chambers arise by differential expansion of regions of the primitive cardiac tube. This process is under the control of specific transcription factors such as Tbx5 and dHAND. To gain insight into the cellular mechanisms that underlie cardiogenesis, we have used a...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171975/ https://www.ncbi.nlm.nih.gov/pubmed/14709543 http://dx.doi.org/10.1083/jcb.200309160 |
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author | Meilhac, Sigolène M. Esner, Milan Kerszberg, Michel Moss, Julie E. Buckingham, Margaret E. |
author_facet | Meilhac, Sigolène M. Esner, Milan Kerszberg, Michel Moss, Julie E. Buckingham, Margaret E. |
author_sort | Meilhac, Sigolène M. |
collection | PubMed |
description | During heart morphogenesis, cardiac chambers arise by differential expansion of regions of the primitive cardiac tube. This process is under the control of specific transcription factors such as Tbx5 and dHAND. To gain insight into the cellular mechanisms that underlie cardiogenesis, we have used a retrospective clonal approach based on the spontaneous recombination of an nlaacZ reporter gene targeted to the murine α-cardiac actin locus. We show that clonal growth of myocardial cells is oriented. At embryonic day (E) 10.5, the shape of clones is characteristic of a given cardiac region and reflects its morphology. This is already detectable in the primitive cardiac tube at E8.5, and is maintained after septation at E14.5 with additional modulations. The clonal analysis reveals new subdivisions of the myocardium, including an interventricular boundary region. Our results show that the myocardium, from the time of its formation, is a polarized and regionalized tissue and point to the role of oriented clonal cell growth in cardiac chamber morphogenesis. |
format | Text |
id | pubmed-2171975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21719752008-03-05 Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis Meilhac, Sigolène M. Esner, Milan Kerszberg, Michel Moss, Julie E. Buckingham, Margaret E. J Cell Biol Article During heart morphogenesis, cardiac chambers arise by differential expansion of regions of the primitive cardiac tube. This process is under the control of specific transcription factors such as Tbx5 and dHAND. To gain insight into the cellular mechanisms that underlie cardiogenesis, we have used a retrospective clonal approach based on the spontaneous recombination of an nlaacZ reporter gene targeted to the murine α-cardiac actin locus. We show that clonal growth of myocardial cells is oriented. At embryonic day (E) 10.5, the shape of clones is characteristic of a given cardiac region and reflects its morphology. This is already detectable in the primitive cardiac tube at E8.5, and is maintained after septation at E14.5 with additional modulations. The clonal analysis reveals new subdivisions of the myocardium, including an interventricular boundary region. Our results show that the myocardium, from the time of its formation, is a polarized and regionalized tissue and point to the role of oriented clonal cell growth in cardiac chamber morphogenesis. The Rockefeller University Press 2004-01-05 /pmc/articles/PMC2171975/ /pubmed/14709543 http://dx.doi.org/10.1083/jcb.200309160 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Meilhac, Sigolène M. Esner, Milan Kerszberg, Michel Moss, Julie E. Buckingham, Margaret E. Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis |
title | Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis |
title_full | Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis |
title_fullStr | Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis |
title_full_unstemmed | Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis |
title_short | Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis |
title_sort | oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171975/ https://www.ncbi.nlm.nih.gov/pubmed/14709543 http://dx.doi.org/10.1083/jcb.200309160 |
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