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Nuclear RNP complex assembly initiates cytoplasmic RNA localization
Cytoplasmic localization of mRNAs is a widespread mechanism for generating cell polarity and can provide the basis for patterning during embryonic development. A prominent example of this is localization of maternal mRNAs in Xenopus oocytes, a process requiring recognition of essential RNA sequences...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172032/ https://www.ncbi.nlm.nih.gov/pubmed/15096527 http://dx.doi.org/10.1083/jcb.200309145 |
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author | Kress, Tracy L. Yoon, Young J. Mowry, Kimberly L. |
author_facet | Kress, Tracy L. Yoon, Young J. Mowry, Kimberly L. |
author_sort | Kress, Tracy L. |
collection | PubMed |
description | Cytoplasmic localization of mRNAs is a widespread mechanism for generating cell polarity and can provide the basis for patterning during embryonic development. A prominent example of this is localization of maternal mRNAs in Xenopus oocytes, a process requiring recognition of essential RNA sequences by protein components of the localization machinery. However, it is not yet clear how and when such protein factors associate with localized RNAs to carry out RNA transport. To trace the RNA–protein interactions that mediate RNA localization, we analyzed RNP complexes from the nucleus and cytoplasm. We find that an early step in the localization pathway is recognition of localized RNAs by specific RNA-binding proteins in the nucleus. After transport into the cytoplasm, the RNP complex is remodeled and additional transport factors are recruited. These results suggest that cytoplasmic RNA localization initiates in the nucleus and that binding of specific RNA-binding proteins in the nucleus may act to target RNAs to their appropriate destinations in the cytoplasm. |
format | Text |
id | pubmed-2172032 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21720322008-03-05 Nuclear RNP complex assembly initiates cytoplasmic RNA localization Kress, Tracy L. Yoon, Young J. Mowry, Kimberly L. J Cell Biol Article Cytoplasmic localization of mRNAs is a widespread mechanism for generating cell polarity and can provide the basis for patterning during embryonic development. A prominent example of this is localization of maternal mRNAs in Xenopus oocytes, a process requiring recognition of essential RNA sequences by protein components of the localization machinery. However, it is not yet clear how and when such protein factors associate with localized RNAs to carry out RNA transport. To trace the RNA–protein interactions that mediate RNA localization, we analyzed RNP complexes from the nucleus and cytoplasm. We find that an early step in the localization pathway is recognition of localized RNAs by specific RNA-binding proteins in the nucleus. After transport into the cytoplasm, the RNP complex is remodeled and additional transport factors are recruited. These results suggest that cytoplasmic RNA localization initiates in the nucleus and that binding of specific RNA-binding proteins in the nucleus may act to target RNAs to their appropriate destinations in the cytoplasm. The Rockefeller University Press 2004-04-26 /pmc/articles/PMC2172032/ /pubmed/15096527 http://dx.doi.org/10.1083/jcb.200309145 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Kress, Tracy L. Yoon, Young J. Mowry, Kimberly L. Nuclear RNP complex assembly initiates cytoplasmic RNA localization |
title | Nuclear RNP complex assembly initiates cytoplasmic RNA localization |
title_full | Nuclear RNP complex assembly initiates cytoplasmic RNA localization |
title_fullStr | Nuclear RNP complex assembly initiates cytoplasmic RNA localization |
title_full_unstemmed | Nuclear RNP complex assembly initiates cytoplasmic RNA localization |
title_short | Nuclear RNP complex assembly initiates cytoplasmic RNA localization |
title_sort | nuclear rnp complex assembly initiates cytoplasmic rna localization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172032/ https://www.ncbi.nlm.nih.gov/pubmed/15096527 http://dx.doi.org/10.1083/jcb.200309145 |
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