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ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts

Reorganization of actomyosin is an essential process for cell migration and myosin regulatory light chain (MLC(20)) phosphorylation plays a key role in this process. Here, we found that zipper-interacting protein (ZIP) kinase plays a predominant role in myosin II phosphorylation in mammalian fibrobl...

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Detalles Bibliográficos
Autores principales: Komatsu, Satoshi, Ikebe, Mitsuo
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172045/
https://www.ncbi.nlm.nih.gov/pubmed/15096528
http://dx.doi.org/10.1083/jcb.200309056
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author Komatsu, Satoshi
Ikebe, Mitsuo
author_facet Komatsu, Satoshi
Ikebe, Mitsuo
author_sort Komatsu, Satoshi
collection PubMed
description Reorganization of actomyosin is an essential process for cell migration and myosin regulatory light chain (MLC(20)) phosphorylation plays a key role in this process. Here, we found that zipper-interacting protein (ZIP) kinase plays a predominant role in myosin II phosphorylation in mammalian fibroblasts. Using two phosphorylation site-specific antibodies, we demonstrated that a significant portion of the phosphorylated MLC(20) is diphosphorylated and that the localization of mono- and diphosphorylated myosin is different from each other. The kinase responsible for the phosphorylation was ZIP kinase because (a) the kinase in the cell extracts phosphorylated Ser19 and Thr18 of MLC(20) with similar potency; (b) immunodepletion of ZIP kinase from the cell extracts markedly diminished its myosin II kinase activity; and (c) disruption of ZIP kinase expression by RNA interference diminished myosin phosphorylation, and resulted in the defect of cell polarity and migration efficiency. These results suggest that ZIP kinase is critical for myosin phosphorylation and necessary for cell motile processes in mammalian fibroblasts.
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spelling pubmed-21720452008-03-05 ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts Komatsu, Satoshi Ikebe, Mitsuo J Cell Biol Article Reorganization of actomyosin is an essential process for cell migration and myosin regulatory light chain (MLC(20)) phosphorylation plays a key role in this process. Here, we found that zipper-interacting protein (ZIP) kinase plays a predominant role in myosin II phosphorylation in mammalian fibroblasts. Using two phosphorylation site-specific antibodies, we demonstrated that a significant portion of the phosphorylated MLC(20) is diphosphorylated and that the localization of mono- and diphosphorylated myosin is different from each other. The kinase responsible for the phosphorylation was ZIP kinase because (a) the kinase in the cell extracts phosphorylated Ser19 and Thr18 of MLC(20) with similar potency; (b) immunodepletion of ZIP kinase from the cell extracts markedly diminished its myosin II kinase activity; and (c) disruption of ZIP kinase expression by RNA interference diminished myosin phosphorylation, and resulted in the defect of cell polarity and migration efficiency. These results suggest that ZIP kinase is critical for myosin phosphorylation and necessary for cell motile processes in mammalian fibroblasts. The Rockefeller University Press 2004-04-26 /pmc/articles/PMC2172045/ /pubmed/15096528 http://dx.doi.org/10.1083/jcb.200309056 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Komatsu, Satoshi
Ikebe, Mitsuo
ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts
title ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts
title_full ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts
title_fullStr ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts
title_full_unstemmed ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts
title_short ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts
title_sort zip kinase is responsible for the phosphorylation of myosin ii and necessary for cell motility in mammalian fibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172045/
https://www.ncbi.nlm.nih.gov/pubmed/15096528
http://dx.doi.org/10.1083/jcb.200309056
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