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p107 regulates neural precursor cells in the mammalian brain

Here we show a novel function for Retinoblastoma family member, p107 in controlling stem cell expansion in the mammalian brain. Adult p107-null mice had elevated numbers of proliferating progenitor cells in their lateral ventricles. In vitro neurosphere assays revealed striking increases in the numb...

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Autores principales: Vanderluit, Jacqueline L., Ferguson, Kerry L., Nikoletopoulou, Vassiliki, Parker, Maura, Ruzhynsky, Vladimir, Alexson, Tania, McNamara, Stephen M., Park, David S., Rudnicki, Michael, Slack, Ruth S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172121/
https://www.ncbi.nlm.nih.gov/pubmed/15353549
http://dx.doi.org/10.1083/jcb.200403156
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author Vanderluit, Jacqueline L.
Ferguson, Kerry L.
Nikoletopoulou, Vassiliki
Parker, Maura
Ruzhynsky, Vladimir
Alexson, Tania
McNamara, Stephen M.
Park, David S.
Rudnicki, Michael
Slack, Ruth S.
author_facet Vanderluit, Jacqueline L.
Ferguson, Kerry L.
Nikoletopoulou, Vassiliki
Parker, Maura
Ruzhynsky, Vladimir
Alexson, Tania
McNamara, Stephen M.
Park, David S.
Rudnicki, Michael
Slack, Ruth S.
author_sort Vanderluit, Jacqueline L.
collection PubMed
description Here we show a novel function for Retinoblastoma family member, p107 in controlling stem cell expansion in the mammalian brain. Adult p107-null mice had elevated numbers of proliferating progenitor cells in their lateral ventricles. In vitro neurosphere assays revealed striking increases in the number of neurosphere forming cells from p107(−/−) brains that exhibited enhanced capacity for self-renewal. An expanded stem cell population in p107-deficient mice was shown in vivo by (a) increased numbers of slowly cycling cells in the lateral ventricles; and (b) accelerated rates of neural precursor repopulation after progenitor ablation. Notch1 was up-regulated in p107(−/−) neurospheres in vitro and brains in vivo. Chromatin immunoprecipitation and p107 overexpression suggest that p107 may modulate the Notch1 pathway. These results demonstrate a novel function for p107 that is distinct from Rb, which is to negatively regulate the number of neural stem cells in the developing and adult brain.
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spelling pubmed-21721212008-03-05 p107 regulates neural precursor cells in the mammalian brain Vanderluit, Jacqueline L. Ferguson, Kerry L. Nikoletopoulou, Vassiliki Parker, Maura Ruzhynsky, Vladimir Alexson, Tania McNamara, Stephen M. Park, David S. Rudnicki, Michael Slack, Ruth S. J Cell Biol Research Articles Here we show a novel function for Retinoblastoma family member, p107 in controlling stem cell expansion in the mammalian brain. Adult p107-null mice had elevated numbers of proliferating progenitor cells in their lateral ventricles. In vitro neurosphere assays revealed striking increases in the number of neurosphere forming cells from p107(−/−) brains that exhibited enhanced capacity for self-renewal. An expanded stem cell population in p107-deficient mice was shown in vivo by (a) increased numbers of slowly cycling cells in the lateral ventricles; and (b) accelerated rates of neural precursor repopulation after progenitor ablation. Notch1 was up-regulated in p107(−/−) neurospheres in vitro and brains in vivo. Chromatin immunoprecipitation and p107 overexpression suggest that p107 may modulate the Notch1 pathway. These results demonstrate a novel function for p107 that is distinct from Rb, which is to negatively regulate the number of neural stem cells in the developing and adult brain. The Rockefeller University Press 2004-09-13 /pmc/articles/PMC2172121/ /pubmed/15353549 http://dx.doi.org/10.1083/jcb.200403156 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Vanderluit, Jacqueline L.
Ferguson, Kerry L.
Nikoletopoulou, Vassiliki
Parker, Maura
Ruzhynsky, Vladimir
Alexson, Tania
McNamara, Stephen M.
Park, David S.
Rudnicki, Michael
Slack, Ruth S.
p107 regulates neural precursor cells in the mammalian brain
title p107 regulates neural precursor cells in the mammalian brain
title_full p107 regulates neural precursor cells in the mammalian brain
title_fullStr p107 regulates neural precursor cells in the mammalian brain
title_full_unstemmed p107 regulates neural precursor cells in the mammalian brain
title_short p107 regulates neural precursor cells in the mammalian brain
title_sort p107 regulates neural precursor cells in the mammalian brain
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172121/
https://www.ncbi.nlm.nih.gov/pubmed/15353549
http://dx.doi.org/10.1083/jcb.200403156
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