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Catch bonds govern adhesion through L-selectin at threshold shear

Flow-enhanced cell adhesion is an unexplained phenomenon that might result from a transport-dependent increase in on-rates or a force-dependent decrease in off-rates of adhesive bonds. L-selectin requires a threshold shear to support leukocyte rolling on P-selectin glycoprotein ligand-1 (PSGL-1) and...

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Autores principales: Yago, Tadayuki, Wu, Jianhua, Wey, C. Diana, Klopocki, Arkadiusz G., Zhu, Cheng, McEver, Rodger P.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172126/
https://www.ncbi.nlm.nih.gov/pubmed/15364963
http://dx.doi.org/10.1083/jcb.200403144
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author Yago, Tadayuki
Wu, Jianhua
Wey, C. Diana
Klopocki, Arkadiusz G.
Zhu, Cheng
McEver, Rodger P.
author_facet Yago, Tadayuki
Wu, Jianhua
Wey, C. Diana
Klopocki, Arkadiusz G.
Zhu, Cheng
McEver, Rodger P.
author_sort Yago, Tadayuki
collection PubMed
description Flow-enhanced cell adhesion is an unexplained phenomenon that might result from a transport-dependent increase in on-rates or a force-dependent decrease in off-rates of adhesive bonds. L-selectin requires a threshold shear to support leukocyte rolling on P-selectin glycoprotein ligand-1 (PSGL-1) and other vascular ligands. Low forces decrease L-selectin–PSGL-1 off-rates (catch bonds), whereas higher forces increase off-rates (slip bonds). We determined that a force-dependent decrease in off-rates dictated flow-enhanced rolling of L-selectin–bearing microspheres or neutrophils on PSGL-1. Catch bonds enabled increasing force to convert short-lived tethers into longer-lived tethers, which decreased rolling velocities and increased the regularity of rolling steps as shear rose from the threshold to an optimal value. As shear increased above the optimum, transitions to slip bonds shortened tether lifetimes, which increased rolling velocities and decreased rolling regularity. Thus, force-dependent alterations of bond lifetimes govern L-selectin–dependent cell adhesion below and above the shear optimum. These findings establish the first biological function for catch bonds as a mechanism for flow-enhanced cell adhesion.
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spelling pubmed-21721262008-03-05 Catch bonds govern adhesion through L-selectin at threshold shear Yago, Tadayuki Wu, Jianhua Wey, C. Diana Klopocki, Arkadiusz G. Zhu, Cheng McEver, Rodger P. J Cell Biol Research Articles Flow-enhanced cell adhesion is an unexplained phenomenon that might result from a transport-dependent increase in on-rates or a force-dependent decrease in off-rates of adhesive bonds. L-selectin requires a threshold shear to support leukocyte rolling on P-selectin glycoprotein ligand-1 (PSGL-1) and other vascular ligands. Low forces decrease L-selectin–PSGL-1 off-rates (catch bonds), whereas higher forces increase off-rates (slip bonds). We determined that a force-dependent decrease in off-rates dictated flow-enhanced rolling of L-selectin–bearing microspheres or neutrophils on PSGL-1. Catch bonds enabled increasing force to convert short-lived tethers into longer-lived tethers, which decreased rolling velocities and increased the regularity of rolling steps as shear rose from the threshold to an optimal value. As shear increased above the optimum, transitions to slip bonds shortened tether lifetimes, which increased rolling velocities and decreased rolling regularity. Thus, force-dependent alterations of bond lifetimes govern L-selectin–dependent cell adhesion below and above the shear optimum. These findings establish the first biological function for catch bonds as a mechanism for flow-enhanced cell adhesion. The Rockefeller University Press 2004-09-13 /pmc/articles/PMC2172126/ /pubmed/15364963 http://dx.doi.org/10.1083/jcb.200403144 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Yago, Tadayuki
Wu, Jianhua
Wey, C. Diana
Klopocki, Arkadiusz G.
Zhu, Cheng
McEver, Rodger P.
Catch bonds govern adhesion through L-selectin at threshold shear
title Catch bonds govern adhesion through L-selectin at threshold shear
title_full Catch bonds govern adhesion through L-selectin at threshold shear
title_fullStr Catch bonds govern adhesion through L-selectin at threshold shear
title_full_unstemmed Catch bonds govern adhesion through L-selectin at threshold shear
title_short Catch bonds govern adhesion through L-selectin at threshold shear
title_sort catch bonds govern adhesion through l-selectin at threshold shear
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172126/
https://www.ncbi.nlm.nih.gov/pubmed/15364963
http://dx.doi.org/10.1083/jcb.200403144
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