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A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5
The cardiac homeobox transcription factor CSX/NKX2-5 plays an important role in vertebrate heart development. Using a yeast two-hybrid screening, we identified a novel LIM domain–containing protein, named CSX-associated LIM protein (Cal), that interacts with CSX/NKX2-5. CSX/NKX2-5 and Cal associate...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172236/ https://www.ncbi.nlm.nih.gov/pubmed/14757752 http://dx.doi.org/10.1083/jcb.200309159 |
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author | Akazawa, Hiroshi Kudoh, Sumiyo Mochizuki, Naoki Takekoshi, Noboru Takano, Hiroyuki Nagai, Toshio Komuro, Issei |
author_facet | Akazawa, Hiroshi Kudoh, Sumiyo Mochizuki, Naoki Takekoshi, Noboru Takano, Hiroyuki Nagai, Toshio Komuro, Issei |
author_sort | Akazawa, Hiroshi |
collection | PubMed |
description | The cardiac homeobox transcription factor CSX/NKX2-5 plays an important role in vertebrate heart development. Using a yeast two-hybrid screening, we identified a novel LIM domain–containing protein, named CSX-associated LIM protein (Cal), that interacts with CSX/NKX2-5. CSX/NKX2-5 and Cal associate with each other both in vivo and in vitro, and the LIM domains of Cal and the homeodomain of CSX/NKX2-5 were necessary for mutual binding. Cal itself possessed the transcription-promoting activity, and cotransfection of Cal enhanced CSX/NKX2-5–induced activation of atrial natriuretic peptide gene promoter. Cal contained a functional nuclear export signal and shuttled from the cytoplasm into the nucleus in response to calcium. Accumulation of Cal in the nucleus of P19CL6 cells promoted myocardial cell differentiation accompanied by increased expression levels of the target genes of CSX/NKX2-5. These results suggest that a novel LIM protein Cal induces cardiomyocyte differentiation through its dynamic intracellular shuttling and association with CSX/NKX2-5. |
format | Text |
id | pubmed-2172236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21722362008-03-05 A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5 Akazawa, Hiroshi Kudoh, Sumiyo Mochizuki, Naoki Takekoshi, Noboru Takano, Hiroyuki Nagai, Toshio Komuro, Issei J Cell Biol Article The cardiac homeobox transcription factor CSX/NKX2-5 plays an important role in vertebrate heart development. Using a yeast two-hybrid screening, we identified a novel LIM domain–containing protein, named CSX-associated LIM protein (Cal), that interacts with CSX/NKX2-5. CSX/NKX2-5 and Cal associate with each other both in vivo and in vitro, and the LIM domains of Cal and the homeodomain of CSX/NKX2-5 were necessary for mutual binding. Cal itself possessed the transcription-promoting activity, and cotransfection of Cal enhanced CSX/NKX2-5–induced activation of atrial natriuretic peptide gene promoter. Cal contained a functional nuclear export signal and shuttled from the cytoplasm into the nucleus in response to calcium. Accumulation of Cal in the nucleus of P19CL6 cells promoted myocardial cell differentiation accompanied by increased expression levels of the target genes of CSX/NKX2-5. These results suggest that a novel LIM protein Cal induces cardiomyocyte differentiation through its dynamic intracellular shuttling and association with CSX/NKX2-5. The Rockefeller University Press 2004-02-02 /pmc/articles/PMC2172236/ /pubmed/14757752 http://dx.doi.org/10.1083/jcb.200309159 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Akazawa, Hiroshi Kudoh, Sumiyo Mochizuki, Naoki Takekoshi, Noboru Takano, Hiroyuki Nagai, Toshio Komuro, Issei A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5 |
title | A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5 |
title_full | A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5 |
title_fullStr | A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5 |
title_full_unstemmed | A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5 |
title_short | A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5 |
title_sort | novel lim protein cal promotes cardiac differentiation by association with csx/nkx2-5 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172236/ https://www.ncbi.nlm.nih.gov/pubmed/14757752 http://dx.doi.org/10.1083/jcb.200309159 |
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