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Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen

Ca(2+) transients trigger many SNARE-dependent membrane fusion events. The homotypic fusion of yeast vacuoles occurs after a release of lumenal Ca(2+). Here, we show that trans-SNARE interactions promote the release of Ca(2+) from the vacuole lumen. Ypt7p–GTP, the Sec1p/Munc18-protein Vps33p, and Rh...

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Autores principales: Merz, Alexey J., Wickner, William T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172329/
https://www.ncbi.nlm.nih.gov/pubmed/14734531
http://dx.doi.org/10.1083/jcb.200310105
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author Merz, Alexey J.
Wickner, William T.
author_facet Merz, Alexey J.
Wickner, William T.
author_sort Merz, Alexey J.
collection PubMed
description Ca(2+) transients trigger many SNARE-dependent membrane fusion events. The homotypic fusion of yeast vacuoles occurs after a release of lumenal Ca(2+). Here, we show that trans-SNARE interactions promote the release of Ca(2+) from the vacuole lumen. Ypt7p–GTP, the Sec1p/Munc18-protein Vps33p, and Rho GTPases, all of which function during docking, are required for Ca(2+) release. Inhibitors of SNARE function prevent Ca(2+) release. Recombinant Vam7p, a soluble Q-SNARE, stimulates Ca(2+) release. Vacuoles lacking either of two complementary SNAREs, Vam3p or Nyv1p, fail to release Ca(2+) upon tethering. Mixing these two vacuole populations together allows Vam3p and Nyv1p to interact in trans and rescues Ca(2+) release. Sec17/18p promote sustained Ca(2+) release by recycling SNAREs (and perhaps other limiting factors), but are not required at the release step itself. We conclude that trans-SNARE assembly events during docking promote Ca(2+) release from the vacuole lumen.
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spelling pubmed-21723292008-03-05 Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen Merz, Alexey J. Wickner, William T. J Cell Biol Article Ca(2+) transients trigger many SNARE-dependent membrane fusion events. The homotypic fusion of yeast vacuoles occurs after a release of lumenal Ca(2+). Here, we show that trans-SNARE interactions promote the release of Ca(2+) from the vacuole lumen. Ypt7p–GTP, the Sec1p/Munc18-protein Vps33p, and Rho GTPases, all of which function during docking, are required for Ca(2+) release. Inhibitors of SNARE function prevent Ca(2+) release. Recombinant Vam7p, a soluble Q-SNARE, stimulates Ca(2+) release. Vacuoles lacking either of two complementary SNAREs, Vam3p or Nyv1p, fail to release Ca(2+) upon tethering. Mixing these two vacuole populations together allows Vam3p and Nyv1p to interact in trans and rescues Ca(2+) release. Sec17/18p promote sustained Ca(2+) release by recycling SNAREs (and perhaps other limiting factors), but are not required at the release step itself. We conclude that trans-SNARE assembly events during docking promote Ca(2+) release from the vacuole lumen. The Rockefeller University Press 2004-01-19 /pmc/articles/PMC2172329/ /pubmed/14734531 http://dx.doi.org/10.1083/jcb.200310105 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Merz, Alexey J.
Wickner, William T.
Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen
title Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen
title_full Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen
title_fullStr Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen
title_full_unstemmed Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen
title_short Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen
title_sort trans-snare interactions elicit ca(2+) efflux from the yeast vacuole lumen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172329/
https://www.ncbi.nlm.nih.gov/pubmed/14734531
http://dx.doi.org/10.1083/jcb.200310105
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