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Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen
Ca(2+) transients trigger many SNARE-dependent membrane fusion events. The homotypic fusion of yeast vacuoles occurs after a release of lumenal Ca(2+). Here, we show that trans-SNARE interactions promote the release of Ca(2+) from the vacuole lumen. Ypt7p–GTP, the Sec1p/Munc18-protein Vps33p, and Rh...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172329/ https://www.ncbi.nlm.nih.gov/pubmed/14734531 http://dx.doi.org/10.1083/jcb.200310105 |
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author | Merz, Alexey J. Wickner, William T. |
author_facet | Merz, Alexey J. Wickner, William T. |
author_sort | Merz, Alexey J. |
collection | PubMed |
description | Ca(2+) transients trigger many SNARE-dependent membrane fusion events. The homotypic fusion of yeast vacuoles occurs after a release of lumenal Ca(2+). Here, we show that trans-SNARE interactions promote the release of Ca(2+) from the vacuole lumen. Ypt7p–GTP, the Sec1p/Munc18-protein Vps33p, and Rho GTPases, all of which function during docking, are required for Ca(2+) release. Inhibitors of SNARE function prevent Ca(2+) release. Recombinant Vam7p, a soluble Q-SNARE, stimulates Ca(2+) release. Vacuoles lacking either of two complementary SNAREs, Vam3p or Nyv1p, fail to release Ca(2+) upon tethering. Mixing these two vacuole populations together allows Vam3p and Nyv1p to interact in trans and rescues Ca(2+) release. Sec17/18p promote sustained Ca(2+) release by recycling SNAREs (and perhaps other limiting factors), but are not required at the release step itself. We conclude that trans-SNARE assembly events during docking promote Ca(2+) release from the vacuole lumen. |
format | Text |
id | pubmed-2172329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21723292008-03-05 Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen Merz, Alexey J. Wickner, William T. J Cell Biol Article Ca(2+) transients trigger many SNARE-dependent membrane fusion events. The homotypic fusion of yeast vacuoles occurs after a release of lumenal Ca(2+). Here, we show that trans-SNARE interactions promote the release of Ca(2+) from the vacuole lumen. Ypt7p–GTP, the Sec1p/Munc18-protein Vps33p, and Rho GTPases, all of which function during docking, are required for Ca(2+) release. Inhibitors of SNARE function prevent Ca(2+) release. Recombinant Vam7p, a soluble Q-SNARE, stimulates Ca(2+) release. Vacuoles lacking either of two complementary SNAREs, Vam3p or Nyv1p, fail to release Ca(2+) upon tethering. Mixing these two vacuole populations together allows Vam3p and Nyv1p to interact in trans and rescues Ca(2+) release. Sec17/18p promote sustained Ca(2+) release by recycling SNAREs (and perhaps other limiting factors), but are not required at the release step itself. We conclude that trans-SNARE assembly events during docking promote Ca(2+) release from the vacuole lumen. The Rockefeller University Press 2004-01-19 /pmc/articles/PMC2172329/ /pubmed/14734531 http://dx.doi.org/10.1083/jcb.200310105 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Merz, Alexey J. Wickner, William T. Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen |
title | Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen |
title_full | Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen |
title_fullStr | Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen |
title_full_unstemmed | Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen |
title_short | Trans-SNARE interactions elicit Ca(2+) efflux from the yeast vacuole lumen |
title_sort | trans-snare interactions elicit ca(2+) efflux from the yeast vacuole lumen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172329/ https://www.ncbi.nlm.nih.gov/pubmed/14734531 http://dx.doi.org/10.1083/jcb.200310105 |
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