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Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release
We have recently isolated a novel cytomatrix at the active zone (CAZ)–associated protein, CAST, and found it directly binds another CAZ protein RIM1 and indirectly binds Munc13-1 through RIM1; RIM1 and Munc13-1 directly bind to each other and are implicated in priming of synaptic vesicles. Here, we...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2004
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172332/ https://www.ncbi.nlm.nih.gov/pubmed/14734538 http://dx.doi.org/10.1083/jcb.200307101 |
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author | Takao-Rikitsu, Etsuko Mochida, Sumiko Inoue, Eiji Deguchi-Tawarada, Maki Inoue, Marie Ohtsuka, Toshihisa Takai, Yoshimi |
author_facet | Takao-Rikitsu, Etsuko Mochida, Sumiko Inoue, Eiji Deguchi-Tawarada, Maki Inoue, Marie Ohtsuka, Toshihisa Takai, Yoshimi |
author_sort | Takao-Rikitsu, Etsuko |
collection | PubMed |
description | We have recently isolated a novel cytomatrix at the active zone (CAZ)–associated protein, CAST, and found it directly binds another CAZ protein RIM1 and indirectly binds Munc13-1 through RIM1; RIM1 and Munc13-1 directly bind to each other and are implicated in priming of synaptic vesicles. Here, we show that all the CAZ proteins thus far known form a large molecular complex in the brain, including CAST, RIM1, Munc13-1, Bassoon, and Piccolo. RIM1 and Bassoon directly bind to the COOH terminus and central region of CAST, respectively, forming a ternary complex. Piccolo, which is structurally related to Bassoon, also binds to the Bassoon-binding region of CAST. Moreover, the microinjected RIM1- or Bassoon-binding region of CAST impairs synaptic transmission in cultured superior cervical ganglion neurons. Furthermore, the CAST-binding domain of RIM1 or Bassoon also impairs synaptic transmission in the cultured neurons. These results indicate that CAST serves as a key component of the CAZ structure and is involved in neurotransmitter release by binding these CAZ proteins. |
format | Text |
id | pubmed-2172332 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21723322008-03-05 Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release Takao-Rikitsu, Etsuko Mochida, Sumiko Inoue, Eiji Deguchi-Tawarada, Maki Inoue, Marie Ohtsuka, Toshihisa Takai, Yoshimi J Cell Biol Article We have recently isolated a novel cytomatrix at the active zone (CAZ)–associated protein, CAST, and found it directly binds another CAZ protein RIM1 and indirectly binds Munc13-1 through RIM1; RIM1 and Munc13-1 directly bind to each other and are implicated in priming of synaptic vesicles. Here, we show that all the CAZ proteins thus far known form a large molecular complex in the brain, including CAST, RIM1, Munc13-1, Bassoon, and Piccolo. RIM1 and Bassoon directly bind to the COOH terminus and central region of CAST, respectively, forming a ternary complex. Piccolo, which is structurally related to Bassoon, also binds to the Bassoon-binding region of CAST. Moreover, the microinjected RIM1- or Bassoon-binding region of CAST impairs synaptic transmission in cultured superior cervical ganglion neurons. Furthermore, the CAST-binding domain of RIM1 or Bassoon also impairs synaptic transmission in the cultured neurons. These results indicate that CAST serves as a key component of the CAZ structure and is involved in neurotransmitter release by binding these CAZ proteins. The Rockefeller University Press 2004-01-19 /pmc/articles/PMC2172332/ /pubmed/14734538 http://dx.doi.org/10.1083/jcb.200307101 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Takao-Rikitsu, Etsuko Mochida, Sumiko Inoue, Eiji Deguchi-Tawarada, Maki Inoue, Marie Ohtsuka, Toshihisa Takai, Yoshimi Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release |
title | Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release |
title_full | Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release |
title_fullStr | Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release |
title_full_unstemmed | Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release |
title_short | Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release |
title_sort | physical and functional interaction of the active zone proteins, cast, rim1, and bassoon, in neurotransmitter release |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172332/ https://www.ncbi.nlm.nih.gov/pubmed/14734538 http://dx.doi.org/10.1083/jcb.200307101 |
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