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Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release

We have recently isolated a novel cytomatrix at the active zone (CAZ)–associated protein, CAST, and found it directly binds another CAZ protein RIM1 and indirectly binds Munc13-1 through RIM1; RIM1 and Munc13-1 directly bind to each other and are implicated in priming of synaptic vesicles. Here, we...

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Autores principales: Takao-Rikitsu, Etsuko, Mochida, Sumiko, Inoue, Eiji, Deguchi-Tawarada, Maki, Inoue, Marie, Ohtsuka, Toshihisa, Takai, Yoshimi
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172332/
https://www.ncbi.nlm.nih.gov/pubmed/14734538
http://dx.doi.org/10.1083/jcb.200307101
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author Takao-Rikitsu, Etsuko
Mochida, Sumiko
Inoue, Eiji
Deguchi-Tawarada, Maki
Inoue, Marie
Ohtsuka, Toshihisa
Takai, Yoshimi
author_facet Takao-Rikitsu, Etsuko
Mochida, Sumiko
Inoue, Eiji
Deguchi-Tawarada, Maki
Inoue, Marie
Ohtsuka, Toshihisa
Takai, Yoshimi
author_sort Takao-Rikitsu, Etsuko
collection PubMed
description We have recently isolated a novel cytomatrix at the active zone (CAZ)–associated protein, CAST, and found it directly binds another CAZ protein RIM1 and indirectly binds Munc13-1 through RIM1; RIM1 and Munc13-1 directly bind to each other and are implicated in priming of synaptic vesicles. Here, we show that all the CAZ proteins thus far known form a large molecular complex in the brain, including CAST, RIM1, Munc13-1, Bassoon, and Piccolo. RIM1 and Bassoon directly bind to the COOH terminus and central region of CAST, respectively, forming a ternary complex. Piccolo, which is structurally related to Bassoon, also binds to the Bassoon-binding region of CAST. Moreover, the microinjected RIM1- or Bassoon-binding region of CAST impairs synaptic transmission in cultured superior cervical ganglion neurons. Furthermore, the CAST-binding domain of RIM1 or Bassoon also impairs synaptic transmission in the cultured neurons. These results indicate that CAST serves as a key component of the CAZ structure and is involved in neurotransmitter release by binding these CAZ proteins.
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spelling pubmed-21723322008-03-05 Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release Takao-Rikitsu, Etsuko Mochida, Sumiko Inoue, Eiji Deguchi-Tawarada, Maki Inoue, Marie Ohtsuka, Toshihisa Takai, Yoshimi J Cell Biol Article We have recently isolated a novel cytomatrix at the active zone (CAZ)–associated protein, CAST, and found it directly binds another CAZ protein RIM1 and indirectly binds Munc13-1 through RIM1; RIM1 and Munc13-1 directly bind to each other and are implicated in priming of synaptic vesicles. Here, we show that all the CAZ proteins thus far known form a large molecular complex in the brain, including CAST, RIM1, Munc13-1, Bassoon, and Piccolo. RIM1 and Bassoon directly bind to the COOH terminus and central region of CAST, respectively, forming a ternary complex. Piccolo, which is structurally related to Bassoon, also binds to the Bassoon-binding region of CAST. Moreover, the microinjected RIM1- or Bassoon-binding region of CAST impairs synaptic transmission in cultured superior cervical ganglion neurons. Furthermore, the CAST-binding domain of RIM1 or Bassoon also impairs synaptic transmission in the cultured neurons. These results indicate that CAST serves as a key component of the CAZ structure and is involved in neurotransmitter release by binding these CAZ proteins. The Rockefeller University Press 2004-01-19 /pmc/articles/PMC2172332/ /pubmed/14734538 http://dx.doi.org/10.1083/jcb.200307101 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Takao-Rikitsu, Etsuko
Mochida, Sumiko
Inoue, Eiji
Deguchi-Tawarada, Maki
Inoue, Marie
Ohtsuka, Toshihisa
Takai, Yoshimi
Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release
title Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release
title_full Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release
title_fullStr Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release
title_full_unstemmed Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release
title_short Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release
title_sort physical and functional interaction of the active zone proteins, cast, rim1, and bassoon, in neurotransmitter release
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172332/
https://www.ncbi.nlm.nih.gov/pubmed/14734538
http://dx.doi.org/10.1083/jcb.200307101
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