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Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators

The dynamic regulation of polarized cell growth allows cells to form structures of defined size and shape. We have studied the regulation of polarized growth using mating yeast as a model. Haploid yeast cells treated with high concentration of pheromone form successive mating projections that initia...

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Detalles Bibliográficos
Autores principales: Bidlingmaier, Scott, Snyder, Michael
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172334/
https://www.ncbi.nlm.nih.gov/pubmed/14734532
http://dx.doi.org/10.1083/jcb.200307065
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author Bidlingmaier, Scott
Snyder, Michael
author_facet Bidlingmaier, Scott
Snyder, Michael
author_sort Bidlingmaier, Scott
collection PubMed
description The dynamic regulation of polarized cell growth allows cells to form structures of defined size and shape. We have studied the regulation of polarized growth using mating yeast as a model. Haploid yeast cells treated with high concentration of pheromone form successive mating projections that initiate and terminate growth with regular periodicity. The mechanisms that control the frequency of growth initiation and termination under these conditions are not well understood. We found that the polarisome components Spa2, Pea2, and Bni1 and the Cdc42 regulators Cdc24 and Bem3 control the timing and frequency of projection formation. Loss of polarisome components and mutation of Cdc24 decrease the frequency of projection formation, while loss of Bem3 increases the frequency of projection formation. We found that polarisome components and the cell fusion proteins Fus1 and Fus2 are important for the termination of projection growth. Our results define the first molecular regulators that control the timing of growth initiation and termination during eukaryotic cell differentiation.
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spelling pubmed-21723342008-03-05 Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators Bidlingmaier, Scott Snyder, Michael J Cell Biol Article The dynamic regulation of polarized cell growth allows cells to form structures of defined size and shape. We have studied the regulation of polarized growth using mating yeast as a model. Haploid yeast cells treated with high concentration of pheromone form successive mating projections that initiate and terminate growth with regular periodicity. The mechanisms that control the frequency of growth initiation and termination under these conditions are not well understood. We found that the polarisome components Spa2, Pea2, and Bni1 and the Cdc42 regulators Cdc24 and Bem3 control the timing and frequency of projection formation. Loss of polarisome components and mutation of Cdc24 decrease the frequency of projection formation, while loss of Bem3 increases the frequency of projection formation. We found that polarisome components and the cell fusion proteins Fus1 and Fus2 are important for the termination of projection growth. Our results define the first molecular regulators that control the timing of growth initiation and termination during eukaryotic cell differentiation. The Rockefeller University Press 2004-01-19 /pmc/articles/PMC2172334/ /pubmed/14734532 http://dx.doi.org/10.1083/jcb.200307065 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Bidlingmaier, Scott
Snyder, Michael
Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators
title Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators
title_full Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators
title_fullStr Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators
title_full_unstemmed Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators
title_short Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators
title_sort regulation of polarized growth initiation and termination cycles by the polarisome and cdc42 regulators
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172334/
https://www.ncbi.nlm.nih.gov/pubmed/14734532
http://dx.doi.org/10.1083/jcb.200307065
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