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Coalignment of plasma membrane channels and protrusions (fibripositors) specifies the parallelism of tendon

The functional properties of tendon require an extracellular matrix (ECM) rich in elongated collagen fibrils in parallel register. We sought to understand how embryonic fibroblasts elaborate this exquisite arrangement of fibrils. We show that procollagen processing and collagen fibrillogenesis are i...

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Autores principales: Canty, Elizabeth G., Lu, Yinhui, Meadows, Roger S., Shaw, Michael K., Holmes, David F., Kadler, Karl E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172348/
https://www.ncbi.nlm.nih.gov/pubmed/15159420
http://dx.doi.org/10.1083/jcb.200312071
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author Canty, Elizabeth G.
Lu, Yinhui
Meadows, Roger S.
Shaw, Michael K.
Holmes, David F.
Kadler, Karl E.
author_facet Canty, Elizabeth G.
Lu, Yinhui
Meadows, Roger S.
Shaw, Michael K.
Holmes, David F.
Kadler, Karl E.
author_sort Canty, Elizabeth G.
collection PubMed
description The functional properties of tendon require an extracellular matrix (ECM) rich in elongated collagen fibrils in parallel register. We sought to understand how embryonic fibroblasts elaborate this exquisite arrangement of fibrils. We show that procollagen processing and collagen fibrillogenesis are initiated in Golgi to plasma membrane carriers (GPCs). These carriers and their cargo of 28-nm-diam fibrils are targeted to previously unidentified plasma membrane (PM) protrusions (here designated “fibripositors”) that are parallel to the tendon axis and project into parallel channels between cells. The base of the fibripositor lumen (buried several microns within the cell) is a nucleation site of collagen fibrillogenesis. The tip of the fibripositor is the site of fibril deposition to the ECM. Fibripositors are absent at postnatal stages when fibrils increase in diameter by accretion of extracellular collagen, thereby maintaining parallelism of the tendon. Thus, we show that the parallelism of tendon is determined by the late secretory pathway and interaction of adjacent PMs to form extracellular channels.
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spelling pubmed-21723482008-03-05 Coalignment of plasma membrane channels and protrusions (fibripositors) specifies the parallelism of tendon Canty, Elizabeth G. Lu, Yinhui Meadows, Roger S. Shaw, Michael K. Holmes, David F. Kadler, Karl E. J Cell Biol Article The functional properties of tendon require an extracellular matrix (ECM) rich in elongated collagen fibrils in parallel register. We sought to understand how embryonic fibroblasts elaborate this exquisite arrangement of fibrils. We show that procollagen processing and collagen fibrillogenesis are initiated in Golgi to plasma membrane carriers (GPCs). These carriers and their cargo of 28-nm-diam fibrils are targeted to previously unidentified plasma membrane (PM) protrusions (here designated “fibripositors”) that are parallel to the tendon axis and project into parallel channels between cells. The base of the fibripositor lumen (buried several microns within the cell) is a nucleation site of collagen fibrillogenesis. The tip of the fibripositor is the site of fibril deposition to the ECM. Fibripositors are absent at postnatal stages when fibrils increase in diameter by accretion of extracellular collagen, thereby maintaining parallelism of the tendon. Thus, we show that the parallelism of tendon is determined by the late secretory pathway and interaction of adjacent PMs to form extracellular channels. The Rockefeller University Press 2004-05-24 /pmc/articles/PMC2172348/ /pubmed/15159420 http://dx.doi.org/10.1083/jcb.200312071 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Canty, Elizabeth G.
Lu, Yinhui
Meadows, Roger S.
Shaw, Michael K.
Holmes, David F.
Kadler, Karl E.
Coalignment of plasma membrane channels and protrusions (fibripositors) specifies the parallelism of tendon
title Coalignment of plasma membrane channels and protrusions (fibripositors) specifies the parallelism of tendon
title_full Coalignment of plasma membrane channels and protrusions (fibripositors) specifies the parallelism of tendon
title_fullStr Coalignment of plasma membrane channels and protrusions (fibripositors) specifies the parallelism of tendon
title_full_unstemmed Coalignment of plasma membrane channels and protrusions (fibripositors) specifies the parallelism of tendon
title_short Coalignment of plasma membrane channels and protrusions (fibripositors) specifies the parallelism of tendon
title_sort coalignment of plasma membrane channels and protrusions (fibripositors) specifies the parallelism of tendon
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172348/
https://www.ncbi.nlm.nih.gov/pubmed/15159420
http://dx.doi.org/10.1083/jcb.200312071
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