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Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut

Enteric neural crest cells (NCCs) migrate and colonize the entire gut and proliferate and differentiate into neurons and glia of the enteric nervous system in vertebrate embryos. We have investigated the mitogenic and morphogenic functions of Sonic hedgehog (Shh) on enteric NCCs in cell and organ cu...

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Detalles Bibliográficos
Autores principales: Fu, Ming, Lui, Vincent Chi Hang, Sham, Mai Har, Pachnis, Vassilis, Tam, Paul Kwong Hang
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172437/
https://www.ncbi.nlm.nih.gov/pubmed/15337776
http://dx.doi.org/10.1083/jcb.200401077
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author Fu, Ming
Lui, Vincent Chi Hang
Sham, Mai Har
Pachnis, Vassilis
Tam, Paul Kwong Hang
author_facet Fu, Ming
Lui, Vincent Chi Hang
Sham, Mai Har
Pachnis, Vassilis
Tam, Paul Kwong Hang
author_sort Fu, Ming
collection PubMed
description Enteric neural crest cells (NCCs) migrate and colonize the entire gut and proliferate and differentiate into neurons and glia of the enteric nervous system in vertebrate embryos. We have investigated the mitogenic and morphogenic functions of Sonic hedgehog (Shh) on enteric NCCs in cell and organ culture. Enteric NCCs expressed Shh receptor Patched and transcripts encoding the Shh signal transducer (Gli1). Shh promoted the proliferation and inhibited the differentiation of NCCs. The pro-neurogenic effect of glial cell line–derived neurotrophic factor (GDNF) on NCCs was abolished by Shh. In gut explants, NCCs migrated from the explants onto the adjacent substratum if GDNF was added, whereas addition of Shh abolished this migration. Neuronal differentiation and coalescence of neural crest–derived cells into myenteric plexuses in explants was repressed by the addition of Shh. Our data suggest that Shh controls the proliferation and differentiation of NCCs and modulates the responsiveness of NCCs toward GDNF inductions.
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spelling pubmed-21724372008-03-05 Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut Fu, Ming Lui, Vincent Chi Hang Sham, Mai Har Pachnis, Vassilis Tam, Paul Kwong Hang J Cell Biol Research Articles Enteric neural crest cells (NCCs) migrate and colonize the entire gut and proliferate and differentiate into neurons and glia of the enteric nervous system in vertebrate embryos. We have investigated the mitogenic and morphogenic functions of Sonic hedgehog (Shh) on enteric NCCs in cell and organ culture. Enteric NCCs expressed Shh receptor Patched and transcripts encoding the Shh signal transducer (Gli1). Shh promoted the proliferation and inhibited the differentiation of NCCs. The pro-neurogenic effect of glial cell line–derived neurotrophic factor (GDNF) on NCCs was abolished by Shh. In gut explants, NCCs migrated from the explants onto the adjacent substratum if GDNF was added, whereas addition of Shh abolished this migration. Neuronal differentiation and coalescence of neural crest–derived cells into myenteric plexuses in explants was repressed by the addition of Shh. Our data suggest that Shh controls the proliferation and differentiation of NCCs and modulates the responsiveness of NCCs toward GDNF inductions. The Rockefeller University Press 2004-08-30 /pmc/articles/PMC2172437/ /pubmed/15337776 http://dx.doi.org/10.1083/jcb.200401077 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Fu, Ming
Lui, Vincent Chi Hang
Sham, Mai Har
Pachnis, Vassilis
Tam, Paul Kwong Hang
Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut
title Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut
title_full Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut
title_fullStr Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut
title_full_unstemmed Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut
title_short Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut
title_sort sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172437/
https://www.ncbi.nlm.nih.gov/pubmed/15337776
http://dx.doi.org/10.1083/jcb.200401077
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