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Cadherin activity is required for activity-induced spine remodeling
Neural activity induces the remodeling of pre- and postsynaptic membranes, which maintain their apposition through cell adhesion molecules. Among them, N-cadherin is redistributed, undergoes activity-dependent conformational changes, and is required for synaptic plasticity. Here, we show that depola...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172468/ https://www.ncbi.nlm.nih.gov/pubmed/15569714 http://dx.doi.org/10.1083/jcb.200406030 |
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author | Okamura, Ko Tanaka, Hidekazu Yagita, Yoshiki Saeki, Yoshinaga Taguchi, Akihiko Hiraoka, Yasushi Zeng, Ling-Hui R Colman, David Miki, Naomasa |
author_facet | Okamura, Ko Tanaka, Hidekazu Yagita, Yoshiki Saeki, Yoshinaga Taguchi, Akihiko Hiraoka, Yasushi Zeng, Ling-Hui R Colman, David Miki, Naomasa |
author_sort | Okamura, Ko |
collection | PubMed |
description | Neural activity induces the remodeling of pre- and postsynaptic membranes, which maintain their apposition through cell adhesion molecules. Among them, N-cadherin is redistributed, undergoes activity-dependent conformational changes, and is required for synaptic plasticity. Here, we show that depolarization induces the enlargement of the width of spine head, and that cadherin activity is essential for this synaptic rearrangement. Dendritic spines visualized with green fluorescent protein in hippocampal neurons showed an expansion by the activation of AMPA receptor, so that the synaptic apposition zone may be expanded. N-cadherin-venus fusion protein laterally dispersed along the expanding spine head. Overexpression of dominant-negative forms of N-cadherin resulted in the abrogation of the spine expansion. Inhibition of actin polymerization with cytochalasin D abolished the spine expansion. Together, our data suggest that cadherin-based adhesion machinery coupled with the actin-cytoskeleton is critical for the remodeling of synaptic apposition zone. |
format | Text |
id | pubmed-2172468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21724682008-03-05 Cadherin activity is required for activity-induced spine remodeling Okamura, Ko Tanaka, Hidekazu Yagita, Yoshiki Saeki, Yoshinaga Taguchi, Akihiko Hiraoka, Yasushi Zeng, Ling-Hui R Colman, David Miki, Naomasa J Cell Biol Research Articles Neural activity induces the remodeling of pre- and postsynaptic membranes, which maintain their apposition through cell adhesion molecules. Among them, N-cadherin is redistributed, undergoes activity-dependent conformational changes, and is required for synaptic plasticity. Here, we show that depolarization induces the enlargement of the width of spine head, and that cadherin activity is essential for this synaptic rearrangement. Dendritic spines visualized with green fluorescent protein in hippocampal neurons showed an expansion by the activation of AMPA receptor, so that the synaptic apposition zone may be expanded. N-cadherin-venus fusion protein laterally dispersed along the expanding spine head. Overexpression of dominant-negative forms of N-cadherin resulted in the abrogation of the spine expansion. Inhibition of actin polymerization with cytochalasin D abolished the spine expansion. Together, our data suggest that cadherin-based adhesion machinery coupled with the actin-cytoskeleton is critical for the remodeling of synaptic apposition zone. The Rockefeller University Press 2004-12-06 /pmc/articles/PMC2172468/ /pubmed/15569714 http://dx.doi.org/10.1083/jcb.200406030 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Okamura, Ko Tanaka, Hidekazu Yagita, Yoshiki Saeki, Yoshinaga Taguchi, Akihiko Hiraoka, Yasushi Zeng, Ling-Hui R Colman, David Miki, Naomasa Cadherin activity is required for activity-induced spine remodeling |
title | Cadherin activity is required for activity-induced spine remodeling |
title_full | Cadherin activity is required for activity-induced spine remodeling |
title_fullStr | Cadherin activity is required for activity-induced spine remodeling |
title_full_unstemmed | Cadherin activity is required for activity-induced spine remodeling |
title_short | Cadherin activity is required for activity-induced spine remodeling |
title_sort | cadherin activity is required for activity-induced spine remodeling |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172468/ https://www.ncbi.nlm.nih.gov/pubmed/15569714 http://dx.doi.org/10.1083/jcb.200406030 |
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