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Cadherin activity is required for activity-induced spine remodeling

Neural activity induces the remodeling of pre- and postsynaptic membranes, which maintain their apposition through cell adhesion molecules. Among them, N-cadherin is redistributed, undergoes activity-dependent conformational changes, and is required for synaptic plasticity. Here, we show that depola...

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Autores principales: Okamura, Ko, Tanaka, Hidekazu, Yagita, Yoshiki, Saeki, Yoshinaga, Taguchi, Akihiko, Hiraoka, Yasushi, Zeng, Ling-Hui, R Colman, David, Miki, Naomasa
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172468/
https://www.ncbi.nlm.nih.gov/pubmed/15569714
http://dx.doi.org/10.1083/jcb.200406030
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author Okamura, Ko
Tanaka, Hidekazu
Yagita, Yoshiki
Saeki, Yoshinaga
Taguchi, Akihiko
Hiraoka, Yasushi
Zeng, Ling-Hui
R Colman, David
Miki, Naomasa
author_facet Okamura, Ko
Tanaka, Hidekazu
Yagita, Yoshiki
Saeki, Yoshinaga
Taguchi, Akihiko
Hiraoka, Yasushi
Zeng, Ling-Hui
R Colman, David
Miki, Naomasa
author_sort Okamura, Ko
collection PubMed
description Neural activity induces the remodeling of pre- and postsynaptic membranes, which maintain their apposition through cell adhesion molecules. Among them, N-cadherin is redistributed, undergoes activity-dependent conformational changes, and is required for synaptic plasticity. Here, we show that depolarization induces the enlargement of the width of spine head, and that cadherin activity is essential for this synaptic rearrangement. Dendritic spines visualized with green fluorescent protein in hippocampal neurons showed an expansion by the activation of AMPA receptor, so that the synaptic apposition zone may be expanded. N-cadherin-venus fusion protein laterally dispersed along the expanding spine head. Overexpression of dominant-negative forms of N-cadherin resulted in the abrogation of the spine expansion. Inhibition of actin polymerization with cytochalasin D abolished the spine expansion. Together, our data suggest that cadherin-based adhesion machinery coupled with the actin-cytoskeleton is critical for the remodeling of synaptic apposition zone.
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spelling pubmed-21724682008-03-05 Cadherin activity is required for activity-induced spine remodeling Okamura, Ko Tanaka, Hidekazu Yagita, Yoshiki Saeki, Yoshinaga Taguchi, Akihiko Hiraoka, Yasushi Zeng, Ling-Hui R Colman, David Miki, Naomasa J Cell Biol Research Articles Neural activity induces the remodeling of pre- and postsynaptic membranes, which maintain their apposition through cell adhesion molecules. Among them, N-cadherin is redistributed, undergoes activity-dependent conformational changes, and is required for synaptic plasticity. Here, we show that depolarization induces the enlargement of the width of spine head, and that cadherin activity is essential for this synaptic rearrangement. Dendritic spines visualized with green fluorescent protein in hippocampal neurons showed an expansion by the activation of AMPA receptor, so that the synaptic apposition zone may be expanded. N-cadherin-venus fusion protein laterally dispersed along the expanding spine head. Overexpression of dominant-negative forms of N-cadherin resulted in the abrogation of the spine expansion. Inhibition of actin polymerization with cytochalasin D abolished the spine expansion. Together, our data suggest that cadherin-based adhesion machinery coupled with the actin-cytoskeleton is critical for the remodeling of synaptic apposition zone. The Rockefeller University Press 2004-12-06 /pmc/articles/PMC2172468/ /pubmed/15569714 http://dx.doi.org/10.1083/jcb.200406030 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Okamura, Ko
Tanaka, Hidekazu
Yagita, Yoshiki
Saeki, Yoshinaga
Taguchi, Akihiko
Hiraoka, Yasushi
Zeng, Ling-Hui
R Colman, David
Miki, Naomasa
Cadherin activity is required for activity-induced spine remodeling
title Cadherin activity is required for activity-induced spine remodeling
title_full Cadherin activity is required for activity-induced spine remodeling
title_fullStr Cadherin activity is required for activity-induced spine remodeling
title_full_unstemmed Cadherin activity is required for activity-induced spine remodeling
title_short Cadherin activity is required for activity-induced spine remodeling
title_sort cadherin activity is required for activity-induced spine remodeling
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172468/
https://www.ncbi.nlm.nih.gov/pubmed/15569714
http://dx.doi.org/10.1083/jcb.200406030
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