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Recycling endosomes can serve as intermediates during transport from the Golgi to the plasma membrane of MDCK cells

The AP-1B clathrin adaptor complex is responsible for the polarized transport of many basolateral membrane proteins in epithelial cells. Localization of AP-1B to recycling endosomes (REs) along with other components (exocyst subunits and Rab8) involved in AP-1B–dependent transport suggested that RE...

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Autores principales: Ang, Agnes Lee, Taguchi, Tomohiko, Francis, Stephen, Fölsch, Heike, Murrells, Lindsay J., Pypaert, Marc, Warren, Graham, Mellman, Ira
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172492/
https://www.ncbi.nlm.nih.gov/pubmed/15534004
http://dx.doi.org/10.1083/jcb.200408165
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author Ang, Agnes Lee
Taguchi, Tomohiko
Francis, Stephen
Fölsch, Heike
Murrells, Lindsay J.
Pypaert, Marc
Warren, Graham
Mellman, Ira
author_facet Ang, Agnes Lee
Taguchi, Tomohiko
Francis, Stephen
Fölsch, Heike
Murrells, Lindsay J.
Pypaert, Marc
Warren, Graham
Mellman, Ira
author_sort Ang, Agnes Lee
collection PubMed
description The AP-1B clathrin adaptor complex is responsible for the polarized transport of many basolateral membrane proteins in epithelial cells. Localization of AP-1B to recycling endosomes (REs) along with other components (exocyst subunits and Rab8) involved in AP-1B–dependent transport suggested that RE might be an intermediate between the Golgi and the plasma membrane. Although the involvement of endosomes in the secretory pathway has long been suspected, we now present direct evidence using four independent methods that REs play a role in basolateral transport in MDCK cells. Newly synthesized AP-1B–dependent cargo, vesicular stomatitis virus glycoprotein G (VSV-G), was found by video microscopy, immunoelectron microscopy, and cell fractionation to enter transferrin-positive REs within a few minutes after exit from the trans-Golgi network. Although transient, RE entry appears essential because enzymatic inactivation of REs blocked VSV-G delivery to the cell surface. Because an apically targeted VSV-G mutant behaved similarly, these results suggest that REs not only serve as an intermediate but also as a common site for polarized sorting on the endocytic and secretory pathways.
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spelling pubmed-21724922008-03-05 Recycling endosomes can serve as intermediates during transport from the Golgi to the plasma membrane of MDCK cells Ang, Agnes Lee Taguchi, Tomohiko Francis, Stephen Fölsch, Heike Murrells, Lindsay J. Pypaert, Marc Warren, Graham Mellman, Ira J Cell Biol Research Articles The AP-1B clathrin adaptor complex is responsible for the polarized transport of many basolateral membrane proteins in epithelial cells. Localization of AP-1B to recycling endosomes (REs) along with other components (exocyst subunits and Rab8) involved in AP-1B–dependent transport suggested that RE might be an intermediate between the Golgi and the plasma membrane. Although the involvement of endosomes in the secretory pathway has long been suspected, we now present direct evidence using four independent methods that REs play a role in basolateral transport in MDCK cells. Newly synthesized AP-1B–dependent cargo, vesicular stomatitis virus glycoprotein G (VSV-G), was found by video microscopy, immunoelectron microscopy, and cell fractionation to enter transferrin-positive REs within a few minutes after exit from the trans-Golgi network. Although transient, RE entry appears essential because enzymatic inactivation of REs blocked VSV-G delivery to the cell surface. Because an apically targeted VSV-G mutant behaved similarly, these results suggest that REs not only serve as an intermediate but also as a common site for polarized sorting on the endocytic and secretory pathways. The Rockefeller University Press 2004-11-08 /pmc/articles/PMC2172492/ /pubmed/15534004 http://dx.doi.org/10.1083/jcb.200408165 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Ang, Agnes Lee
Taguchi, Tomohiko
Francis, Stephen
Fölsch, Heike
Murrells, Lindsay J.
Pypaert, Marc
Warren, Graham
Mellman, Ira
Recycling endosomes can serve as intermediates during transport from the Golgi to the plasma membrane of MDCK cells
title Recycling endosomes can serve as intermediates during transport from the Golgi to the plasma membrane of MDCK cells
title_full Recycling endosomes can serve as intermediates during transport from the Golgi to the plasma membrane of MDCK cells
title_fullStr Recycling endosomes can serve as intermediates during transport from the Golgi to the plasma membrane of MDCK cells
title_full_unstemmed Recycling endosomes can serve as intermediates during transport from the Golgi to the plasma membrane of MDCK cells
title_short Recycling endosomes can serve as intermediates during transport from the Golgi to the plasma membrane of MDCK cells
title_sort recycling endosomes can serve as intermediates during transport from the golgi to the plasma membrane of mdck cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172492/
https://www.ncbi.nlm.nih.gov/pubmed/15534004
http://dx.doi.org/10.1083/jcb.200408165
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