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A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites

Membrane skeletons are structural elements that provide mechanical support to the plasma membrane and define cell shape. Here, we identify and characterize a putative protein component of the membrane skeleton of the malaria parasite. The protein, named PbIMC1a, is the structural orthologue of the T...

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Detalles Bibliográficos
Autores principales: Khater, Emad I., Sinden, Robert E., Dessens, Johannes T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172497/
https://www.ncbi.nlm.nih.gov/pubmed/15533999
http://dx.doi.org/10.1083/jcb.200406068
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author Khater, Emad I.
Sinden, Robert E.
Dessens, Johannes T.
author_facet Khater, Emad I.
Sinden, Robert E.
Dessens, Johannes T.
author_sort Khater, Emad I.
collection PubMed
description Membrane skeletons are structural elements that provide mechanical support to the plasma membrane and define cell shape. Here, we identify and characterize a putative protein component of the membrane skeleton of the malaria parasite. The protein, named PbIMC1a, is the structural orthologue of the Toxoplasma gondii inner membrane complex protein 1 (TgIMC1), a component of the membrane skeleton in tachyzoites. Using targeted gene disruption in the rodent malaria species Plasmodium berghei, we show that PbIMC1a is involved in sporozoite development, is necessary for providing normal sporozoite cell shape and mechanical stability, and is essential for sporozoite infectivity in insect and vertebrate hosts. Knockout of PbIMC1a protein expression reduces, but does not abolish, sporozoite gliding locomotion. We identify a family of proteins related to PbIMC1a in Plasmodium and other apicomplexan parasites. These results provide new functional insight in the role of membrane skeletons in apicomplexan parasite biology.
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spelling pubmed-21724972008-03-05 A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites Khater, Emad I. Sinden, Robert E. Dessens, Johannes T. J Cell Biol Research Articles Membrane skeletons are structural elements that provide mechanical support to the plasma membrane and define cell shape. Here, we identify and characterize a putative protein component of the membrane skeleton of the malaria parasite. The protein, named PbIMC1a, is the structural orthologue of the Toxoplasma gondii inner membrane complex protein 1 (TgIMC1), a component of the membrane skeleton in tachyzoites. Using targeted gene disruption in the rodent malaria species Plasmodium berghei, we show that PbIMC1a is involved in sporozoite development, is necessary for providing normal sporozoite cell shape and mechanical stability, and is essential for sporozoite infectivity in insect and vertebrate hosts. Knockout of PbIMC1a protein expression reduces, but does not abolish, sporozoite gliding locomotion. We identify a family of proteins related to PbIMC1a in Plasmodium and other apicomplexan parasites. These results provide new functional insight in the role of membrane skeletons in apicomplexan parasite biology. The Rockefeller University Press 2004-11-08 /pmc/articles/PMC2172497/ /pubmed/15533999 http://dx.doi.org/10.1083/jcb.200406068 Text en Copyright © 2004, The Rockefeller University Press https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/ (https://creativecommons.org/licenses/by-nc-sa/4.0/) ).
spellingShingle Research Articles
Khater, Emad I.
Sinden, Robert E.
Dessens, Johannes T.
A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites
title A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites
title_full A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites
title_fullStr A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites
title_full_unstemmed A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites
title_short A malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites
title_sort malaria membrane skeletal protein is essential for normal morphogenesis, motility, and infectivity of sporozoites
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172497/
https://www.ncbi.nlm.nih.gov/pubmed/15533999
http://dx.doi.org/10.1083/jcb.200406068
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