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A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them
The basic route and mechanisms for leukocyte migration across the endothelium remain poorly defined. We provide definitive evidence for transcellular (i.e., through individual endothelial cells) diapedesis in vitro and demonstrate that virtually all, both para- and transcellular, diapedesis occurs i...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2004
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172560/ https://www.ncbi.nlm.nih.gov/pubmed/15504916 http://dx.doi.org/10.1083/jcb.200404129 |
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author | Carman, Christopher V. Springer, Timothy A. |
author_facet | Carman, Christopher V. Springer, Timothy A. |
author_sort | Carman, Christopher V. |
collection | PubMed |
description | The basic route and mechanisms for leukocyte migration across the endothelium remain poorly defined. We provide definitive evidence for transcellular (i.e., through individual endothelial cells) diapedesis in vitro and demonstrate that virtually all, both para- and transcellular, diapedesis occurs in the context of a novel “cuplike” transmigratory structure. This endothelial structure was comprised of highly intercellular adhesion molecule-1– and vascular cell adhesion molecule-1–enriched vertical microvilli-like projections that surrounded transmigrating leukocytes and drove redistribution of their integrins into linear tracks oriented parallel to the direction of diapedesis. Disruption of projections was highly correlated with inhibition of transmigration. These findings suggest a novel mechanism, the “transmigratory cup”, by which the endothelium provides directional guidance to leukocytes for extravasation. |
format | Text |
id | pubmed-2172560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21725602008-03-05 A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them Carman, Christopher V. Springer, Timothy A. J Cell Biol Research Articles The basic route and mechanisms for leukocyte migration across the endothelium remain poorly defined. We provide definitive evidence for transcellular (i.e., through individual endothelial cells) diapedesis in vitro and demonstrate that virtually all, both para- and transcellular, diapedesis occurs in the context of a novel “cuplike” transmigratory structure. This endothelial structure was comprised of highly intercellular adhesion molecule-1– and vascular cell adhesion molecule-1–enriched vertical microvilli-like projections that surrounded transmigrating leukocytes and drove redistribution of their integrins into linear tracks oriented parallel to the direction of diapedesis. Disruption of projections was highly correlated with inhibition of transmigration. These findings suggest a novel mechanism, the “transmigratory cup”, by which the endothelium provides directional guidance to leukocytes for extravasation. The Rockefeller University Press 2004-10-25 /pmc/articles/PMC2172560/ /pubmed/15504916 http://dx.doi.org/10.1083/jcb.200404129 Text en Copyright © 2004, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Carman, Christopher V. Springer, Timothy A. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them |
title | A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them |
title_full | A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them |
title_fullStr | A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them |
title_full_unstemmed | A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them |
title_short | A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them |
title_sort | transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172560/ https://www.ncbi.nlm.nih.gov/pubmed/15504916 http://dx.doi.org/10.1083/jcb.200404129 |
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