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Regulation of the expression and processing of caspase-12
Phylogenetic analysis clusters caspase-12 with the inflammatory caspases 1 and 11. We analyzed the expression of caspase-12 in mouse embryos, adult organs, and different cell types and tested the effect of interferons (IFNs) and other proinflammatory stimuli. Constitutive expression of the caspase-1...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172698/ https://www.ncbi.nlm.nih.gov/pubmed/12885762 http://dx.doi.org/10.1083/jcb.200303157 |
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author | Kalai, Michael Lamkanfi, Mohamed Denecker, Geertrui Boogmans, Michael Lippens, Saskia Meeus, Ann Declercq, Wim Vandenabeele, Peter |
author_facet | Kalai, Michael Lamkanfi, Mohamed Denecker, Geertrui Boogmans, Michael Lippens, Saskia Meeus, Ann Declercq, Wim Vandenabeele, Peter |
author_sort | Kalai, Michael |
collection | PubMed |
description | Phylogenetic analysis clusters caspase-12 with the inflammatory caspases 1 and 11. We analyzed the expression of caspase-12 in mouse embryos, adult organs, and different cell types and tested the effect of interferons (IFNs) and other proinflammatory stimuli. Constitutive expression of the caspase-12 protein was restricted to certain cell types, such as epithelial cells, primary fibroblasts, and L929 fibrosarcoma cells. In fibroblasts and B16/B16 melanoma cells, caspase-12 expression is stimulated by IFN-γ but not by IFN-α or -β. The effect is increased further when IFN-γ is combined with TNF, lipopolysaccharide (LPS), or dsRNA. These stimuli also induce caspase-1 and -11 but inhibit the expression of caspase-3 and -9. In contrast to caspase-1 and -11, no caspase-12 protein was detected in macrophages in any of these treatments. Transient overexpression of full-length caspase-12 leads to proteolytic processing of the enzyme and apoptosis. Similar processing occurs in TNF-, LPS-, Fas ligand–, and thapsigargin (Tg)-induced apoptosis. However, B16/B16 melanoma cells die when treated with the ER stress–inducing agent Tg whether they express caspase-12 or not. |
format | Text |
id | pubmed-2172698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21726982008-05-01 Regulation of the expression and processing of caspase-12 Kalai, Michael Lamkanfi, Mohamed Denecker, Geertrui Boogmans, Michael Lippens, Saskia Meeus, Ann Declercq, Wim Vandenabeele, Peter J Cell Biol Article Phylogenetic analysis clusters caspase-12 with the inflammatory caspases 1 and 11. We analyzed the expression of caspase-12 in mouse embryos, adult organs, and different cell types and tested the effect of interferons (IFNs) and other proinflammatory stimuli. Constitutive expression of the caspase-12 protein was restricted to certain cell types, such as epithelial cells, primary fibroblasts, and L929 fibrosarcoma cells. In fibroblasts and B16/B16 melanoma cells, caspase-12 expression is stimulated by IFN-γ but not by IFN-α or -β. The effect is increased further when IFN-γ is combined with TNF, lipopolysaccharide (LPS), or dsRNA. These stimuli also induce caspase-1 and -11 but inhibit the expression of caspase-3 and -9. In contrast to caspase-1 and -11, no caspase-12 protein was detected in macrophages in any of these treatments. Transient overexpression of full-length caspase-12 leads to proteolytic processing of the enzyme and apoptosis. Similar processing occurs in TNF-, LPS-, Fas ligand–, and thapsigargin (Tg)-induced apoptosis. However, B16/B16 melanoma cells die when treated with the ER stress–inducing agent Tg whether they express caspase-12 or not. The Rockefeller University Press 2003-08-04 /pmc/articles/PMC2172698/ /pubmed/12885762 http://dx.doi.org/10.1083/jcb.200303157 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Kalai, Michael Lamkanfi, Mohamed Denecker, Geertrui Boogmans, Michael Lippens, Saskia Meeus, Ann Declercq, Wim Vandenabeele, Peter Regulation of the expression and processing of caspase-12 |
title | Regulation of the expression and processing of caspase-12 |
title_full | Regulation of the expression and processing of caspase-12 |
title_fullStr | Regulation of the expression and processing of caspase-12 |
title_full_unstemmed | Regulation of the expression and processing of caspase-12 |
title_short | Regulation of the expression and processing of caspase-12 |
title_sort | regulation of the expression and processing of caspase-12 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172698/ https://www.ncbi.nlm.nih.gov/pubmed/12885762 http://dx.doi.org/10.1083/jcb.200303157 |
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