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Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae

Rho1p, an essential Rho-type GTPase in Saccharomyces cerevisiae, activates its effectors in the GTP-bound form. Here, we show that Rho1p in secretory vesicles cannot activate 1,3-β-glucan synthase, a cell wall synthesizing enzyme, during vesicular transport to the plasma membrane. Analyses with an a...

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Detalles Bibliográficos
Autores principales: Abe, Mitsuhiro, Qadota, Hiroshi, Hirata, Aiko, Ohya, Yoshikazu
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172714/
https://www.ncbi.nlm.nih.gov/pubmed/12847085
http://dx.doi.org/10.1083/jcb.200301022
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author Abe, Mitsuhiro
Qadota, Hiroshi
Hirata, Aiko
Ohya, Yoshikazu
author_facet Abe, Mitsuhiro
Qadota, Hiroshi
Hirata, Aiko
Ohya, Yoshikazu
author_sort Abe, Mitsuhiro
collection PubMed
description Rho1p, an essential Rho-type GTPase in Saccharomyces cerevisiae, activates its effectors in the GTP-bound form. Here, we show that Rho1p in secretory vesicles cannot activate 1,3-β-glucan synthase, a cell wall synthesizing enzyme, during vesicular transport to the plasma membrane. Analyses with an antibody preferentially reacting with the GTP-bound form of Rho1p revealed that Rho1p remains in the inactive form in secretory vesicles. Rom2p, the GDP/GTP exchange factor of Rho1p, is preferentially localized on the plasma membrane even when vesicular transport is blocked. Overexpression of Rom2p results in delocalization of Rom2p and accumulation of 1,3-β-glucan in secretory vesicles. Based on these results, we propose that Rho1p is kept inactive in intracellular secretory organelles, resulting in repression of the activity of the cell wall–synthesizing enzyme within cells.
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spelling pubmed-21727142008-05-01 Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae Abe, Mitsuhiro Qadota, Hiroshi Hirata, Aiko Ohya, Yoshikazu J Cell Biol Article Rho1p, an essential Rho-type GTPase in Saccharomyces cerevisiae, activates its effectors in the GTP-bound form. Here, we show that Rho1p in secretory vesicles cannot activate 1,3-β-glucan synthase, a cell wall synthesizing enzyme, during vesicular transport to the plasma membrane. Analyses with an antibody preferentially reacting with the GTP-bound form of Rho1p revealed that Rho1p remains in the inactive form in secretory vesicles. Rom2p, the GDP/GTP exchange factor of Rho1p, is preferentially localized on the plasma membrane even when vesicular transport is blocked. Overexpression of Rom2p results in delocalization of Rom2p and accumulation of 1,3-β-glucan in secretory vesicles. Based on these results, we propose that Rho1p is kept inactive in intracellular secretory organelles, resulting in repression of the activity of the cell wall–synthesizing enzyme within cells. The Rockefeller University Press 2003-07-07 /pmc/articles/PMC2172714/ /pubmed/12847085 http://dx.doi.org/10.1083/jcb.200301022 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Abe, Mitsuhiro
Qadota, Hiroshi
Hirata, Aiko
Ohya, Yoshikazu
Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae
title Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae
title_full Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae
title_fullStr Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae
title_full_unstemmed Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae
title_short Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae
title_sort lack of gtp-bound rho1p in secretory vesicles of saccharomyces cerevisiae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172714/
https://www.ncbi.nlm.nih.gov/pubmed/12847085
http://dx.doi.org/10.1083/jcb.200301022
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