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Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head
Post-Golgi carriers of various newly synthesized axonal membrane proteins, which possess kinesin (KIF5)-driven highly processive motility, were transported from the TGN directly to axons. We found that KIF5 has a preference to the microtubules in the initial segment of axon. Low dose paclitaxel trea...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2003
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172855/ https://www.ncbi.nlm.nih.gov/pubmed/12975348 http://dx.doi.org/10.1083/jcb.200302175 |
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author | Nakata, Takao Hirokawa, Nobutaka |
author_facet | Nakata, Takao Hirokawa, Nobutaka |
author_sort | Nakata, Takao |
collection | PubMed |
description | Post-Golgi carriers of various newly synthesized axonal membrane proteins, which possess kinesin (KIF5)-driven highly processive motility, were transported from the TGN directly to axons. We found that KIF5 has a preference to the microtubules in the initial segment of axon. Low dose paclitaxel treatment caused missorting of KIF5, as well as axonal membrane proteins to the tips of dendrites. Microtubules in the initial segment of axons showed a remarkably high affinity to EB1–YFP, which was known to bind the tips of growing microtubules. These findings revealed unique features of the microtubule cytoskeletons in the initial segment, and suggested that they provide directional information for polarized axonal transport. |
format | Text |
id | pubmed-2172855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21728552008-05-01 Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head Nakata, Takao Hirokawa, Nobutaka J Cell Biol Article Post-Golgi carriers of various newly synthesized axonal membrane proteins, which possess kinesin (KIF5)-driven highly processive motility, were transported from the TGN directly to axons. We found that KIF5 has a preference to the microtubules in the initial segment of axon. Low dose paclitaxel treatment caused missorting of KIF5, as well as axonal membrane proteins to the tips of dendrites. Microtubules in the initial segment of axons showed a remarkably high affinity to EB1–YFP, which was known to bind the tips of growing microtubules. These findings revealed unique features of the microtubule cytoskeletons in the initial segment, and suggested that they provide directional information for polarized axonal transport. The Rockefeller University Press 2003-09-15 /pmc/articles/PMC2172855/ /pubmed/12975348 http://dx.doi.org/10.1083/jcb.200302175 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Nakata, Takao Hirokawa, Nobutaka Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head |
title | Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head |
title_full | Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head |
title_fullStr | Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head |
title_full_unstemmed | Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head |
title_short | Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head |
title_sort | microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172855/ https://www.ncbi.nlm.nih.gov/pubmed/12975348 http://dx.doi.org/10.1083/jcb.200302175 |
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