RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis

Amitotically activated mitogen-activated protein kinase 1 (MEK1) fragments the pericentriolar Golgi stacks in mammalian cells. We show that activated MEK1 is found on the Golgi apparatus in late prophase. The fragmented and dispersed Golgi membranes in prometaphase and later stages of mitosis do not...

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Detalles Bibliográficos
Autores principales: Colanzi, Antonino, Sutterlin, Christine, Malhotra, Vivek
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172875/
https://www.ncbi.nlm.nih.gov/pubmed/12695496
http://dx.doi.org/10.1083/jcb.200208099
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author Colanzi, Antonino
Sutterlin, Christine
Malhotra, Vivek
author_facet Colanzi, Antonino
Sutterlin, Christine
Malhotra, Vivek
author_sort Colanzi, Antonino
collection PubMed
description Amitotically activated mitogen-activated protein kinase 1 (MEK1) fragments the pericentriolar Golgi stacks in mammalian cells. We show that activated MEK1 is found on the Golgi apparatus in late prophase. The fragmented and dispersed Golgi membranes in prometaphase and later stages of mitosis do not contain activated MEK1. MEK1-dependent Golgi complex fragmentation is through activation by RAF1 and not MEK1 kinase 1. We propose that a RAF1-dependent activation of MEK1 and its presence on the Golgi apparatus in late prophase is required for Golgi complex fragmentation.
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spelling pubmed-21728752008-05-01 RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis Colanzi, Antonino Sutterlin, Christine Malhotra, Vivek J Cell Biol Report Amitotically activated mitogen-activated protein kinase 1 (MEK1) fragments the pericentriolar Golgi stacks in mammalian cells. We show that activated MEK1 is found on the Golgi apparatus in late prophase. The fragmented and dispersed Golgi membranes in prometaphase and later stages of mitosis do not contain activated MEK1. MEK1-dependent Golgi complex fragmentation is through activation by RAF1 and not MEK1 kinase 1. We propose that a RAF1-dependent activation of MEK1 and its presence on the Golgi apparatus in late prophase is required for Golgi complex fragmentation. The Rockefeller University Press 2003-04-14 /pmc/articles/PMC2172875/ /pubmed/12695496 http://dx.doi.org/10.1083/jcb.200208099 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Report
Colanzi, Antonino
Sutterlin, Christine
Malhotra, Vivek
RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis
title RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis
title_full RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis
title_fullStr RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis
title_full_unstemmed RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis
title_short RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis
title_sort raf1-activated mek1 is found on the golgi apparatus in late prophase and is required for golgi complex fragmentation in mitosis
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172875/
https://www.ncbi.nlm.nih.gov/pubmed/12695496
http://dx.doi.org/10.1083/jcb.200208099
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AT sutterlinchristine raf1activatedmek1isfoundonthegolgiapparatusinlateprophaseandisrequiredforgolgicomplexfragmentationinmitosis
AT malhotravivek raf1activatedmek1isfoundonthegolgiapparatusinlateprophaseandisrequiredforgolgicomplexfragmentationinmitosis

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