Cargando…

Chromosomes are predominantly located randomly with respect to each other in interphase human cells

To test quantitatively whether there are systematic chromosome–chromosome associations within human interphase nuclei, interchanges between all possible heterologous pairs of chromosomes were measured with 24-color whole-chromosome painting (multiplex FISH), after damage to interphase lymphocytes by...

Descripción completa

Detalles Bibliográficos
Autores principales: Cornforth, Michael N., Greulich-Bode, Karin M., Loucas, Bradford D., Arsuaga, Javier, Vázquez, Mariel, Sachs, Rainer K., Brückner, Martina, Molls, Michael, Hahnfeldt, Philip, Hlatky, Lynn, Brenner, David J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173058/
https://www.ncbi.nlm.nih.gov/pubmed/12403811
http://dx.doi.org/10.1083/jcb.200206009
_version_ 1782145149170614272
author Cornforth, Michael N.
Greulich-Bode, Karin M.
Loucas, Bradford D.
Arsuaga, Javier
Vázquez, Mariel
Sachs, Rainer K.
Brückner, Martina
Molls, Michael
Hahnfeldt, Philip
Hlatky, Lynn
Brenner, David J.
author_facet Cornforth, Michael N.
Greulich-Bode, Karin M.
Loucas, Bradford D.
Arsuaga, Javier
Vázquez, Mariel
Sachs, Rainer K.
Brückner, Martina
Molls, Michael
Hahnfeldt, Philip
Hlatky, Lynn
Brenner, David J.
author_sort Cornforth, Michael N.
collection PubMed
description To test quantitatively whether there are systematic chromosome–chromosome associations within human interphase nuclei, interchanges between all possible heterologous pairs of chromosomes were measured with 24-color whole-chromosome painting (multiplex FISH), after damage to interphase lymphocytes by sparsely ionizing radiation in vitro. An excess of interchanges for a specific chromosome pair would indicate spatial proximity between the chromosomes comprising that pair. The experimental design was such that quite small deviations from randomness (extra pairwise interchanges within a group of chromosomes) would be detectable. The only statistically significant chromosome cluster was a group of five chromosomes previously observed to be preferentially located near the center of the nucleus. However, quantitatively, the overall deviation from randomness within the whole genome was small. Thus, whereas some chromosome–chromosome associations are clearly present, at the whole-chromosomal level, the predominant overall pattern appears to be spatially random.
format Text
id pubmed-2173058
institution National Center for Biotechnology Information
language English
publishDate 2002
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21730582008-05-01 Chromosomes are predominantly located randomly with respect to each other in interphase human cells Cornforth, Michael N. Greulich-Bode, Karin M. Loucas, Bradford D. Arsuaga, Javier Vázquez, Mariel Sachs, Rainer K. Brückner, Martina Molls, Michael Hahnfeldt, Philip Hlatky, Lynn Brenner, David J. J Cell Biol Article To test quantitatively whether there are systematic chromosome–chromosome associations within human interphase nuclei, interchanges between all possible heterologous pairs of chromosomes were measured with 24-color whole-chromosome painting (multiplex FISH), after damage to interphase lymphocytes by sparsely ionizing radiation in vitro. An excess of interchanges for a specific chromosome pair would indicate spatial proximity between the chromosomes comprising that pair. The experimental design was such that quite small deviations from randomness (extra pairwise interchanges within a group of chromosomes) would be detectable. The only statistically significant chromosome cluster was a group of five chromosomes previously observed to be preferentially located near the center of the nucleus. However, quantitatively, the overall deviation from randomness within the whole genome was small. Thus, whereas some chromosome–chromosome associations are clearly present, at the whole-chromosomal level, the predominant overall pattern appears to be spatially random. The Rockefeller University Press 2002-10-28 /pmc/articles/PMC2173058/ /pubmed/12403811 http://dx.doi.org/10.1083/jcb.200206009 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Cornforth, Michael N.
Greulich-Bode, Karin M.
Loucas, Bradford D.
Arsuaga, Javier
Vázquez, Mariel
Sachs, Rainer K.
Brückner, Martina
Molls, Michael
Hahnfeldt, Philip
Hlatky, Lynn
Brenner, David J.
Chromosomes are predominantly located randomly with respect to each other in interphase human cells
title Chromosomes are predominantly located randomly with respect to each other in interphase human cells
title_full Chromosomes are predominantly located randomly with respect to each other in interphase human cells
title_fullStr Chromosomes are predominantly located randomly with respect to each other in interphase human cells
title_full_unstemmed Chromosomes are predominantly located randomly with respect to each other in interphase human cells
title_short Chromosomes are predominantly located randomly with respect to each other in interphase human cells
title_sort chromosomes are predominantly located randomly with respect to each other in interphase human cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173058/
https://www.ncbi.nlm.nih.gov/pubmed/12403811
http://dx.doi.org/10.1083/jcb.200206009
work_keys_str_mv AT cornforthmichaeln chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells
AT greulichbodekarinm chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells
AT loucasbradfordd chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells
AT arsuagajavier chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells
AT vazquezmariel chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells
AT sachsrainerk chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells
AT brucknermartina chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells
AT mollsmichael chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells
AT hahnfeldtphilip chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells
AT hlatkylynn chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells
AT brennerdavidj chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells