Cargando…
Chromosomes are predominantly located randomly with respect to each other in interphase human cells
To test quantitatively whether there are systematic chromosome–chromosome associations within human interphase nuclei, interchanges between all possible heterologous pairs of chromosomes were measured with 24-color whole-chromosome painting (multiplex FISH), after damage to interphase lymphocytes by...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2002
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173058/ https://www.ncbi.nlm.nih.gov/pubmed/12403811 http://dx.doi.org/10.1083/jcb.200206009 |
_version_ | 1782145149170614272 |
---|---|
author | Cornforth, Michael N. Greulich-Bode, Karin M. Loucas, Bradford D. Arsuaga, Javier Vázquez, Mariel Sachs, Rainer K. Brückner, Martina Molls, Michael Hahnfeldt, Philip Hlatky, Lynn Brenner, David J. |
author_facet | Cornforth, Michael N. Greulich-Bode, Karin M. Loucas, Bradford D. Arsuaga, Javier Vázquez, Mariel Sachs, Rainer K. Brückner, Martina Molls, Michael Hahnfeldt, Philip Hlatky, Lynn Brenner, David J. |
author_sort | Cornforth, Michael N. |
collection | PubMed |
description | To test quantitatively whether there are systematic chromosome–chromosome associations within human interphase nuclei, interchanges between all possible heterologous pairs of chromosomes were measured with 24-color whole-chromosome painting (multiplex FISH), after damage to interphase lymphocytes by sparsely ionizing radiation in vitro. An excess of interchanges for a specific chromosome pair would indicate spatial proximity between the chromosomes comprising that pair. The experimental design was such that quite small deviations from randomness (extra pairwise interchanges within a group of chromosomes) would be detectable. The only statistically significant chromosome cluster was a group of five chromosomes previously observed to be preferentially located near the center of the nucleus. However, quantitatively, the overall deviation from randomness within the whole genome was small. Thus, whereas some chromosome–chromosome associations are clearly present, at the whole-chromosomal level, the predominant overall pattern appears to be spatially random. |
format | Text |
id | pubmed-2173058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21730582008-05-01 Chromosomes are predominantly located randomly with respect to each other in interphase human cells Cornforth, Michael N. Greulich-Bode, Karin M. Loucas, Bradford D. Arsuaga, Javier Vázquez, Mariel Sachs, Rainer K. Brückner, Martina Molls, Michael Hahnfeldt, Philip Hlatky, Lynn Brenner, David J. J Cell Biol Article To test quantitatively whether there are systematic chromosome–chromosome associations within human interphase nuclei, interchanges between all possible heterologous pairs of chromosomes were measured with 24-color whole-chromosome painting (multiplex FISH), after damage to interphase lymphocytes by sparsely ionizing radiation in vitro. An excess of interchanges for a specific chromosome pair would indicate spatial proximity between the chromosomes comprising that pair. The experimental design was such that quite small deviations from randomness (extra pairwise interchanges within a group of chromosomes) would be detectable. The only statistically significant chromosome cluster was a group of five chromosomes previously observed to be preferentially located near the center of the nucleus. However, quantitatively, the overall deviation from randomness within the whole genome was small. Thus, whereas some chromosome–chromosome associations are clearly present, at the whole-chromosomal level, the predominant overall pattern appears to be spatially random. The Rockefeller University Press 2002-10-28 /pmc/articles/PMC2173058/ /pubmed/12403811 http://dx.doi.org/10.1083/jcb.200206009 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Cornforth, Michael N. Greulich-Bode, Karin M. Loucas, Bradford D. Arsuaga, Javier Vázquez, Mariel Sachs, Rainer K. Brückner, Martina Molls, Michael Hahnfeldt, Philip Hlatky, Lynn Brenner, David J. Chromosomes are predominantly located randomly with respect to each other in interphase human cells |
title | Chromosomes are predominantly located randomly with respect to each other in interphase human cells |
title_full | Chromosomes are predominantly located randomly with respect to each other in interphase human cells |
title_fullStr | Chromosomes are predominantly located randomly with respect to each other in interphase human cells |
title_full_unstemmed | Chromosomes are predominantly located randomly with respect to each other in interphase human cells |
title_short | Chromosomes are predominantly located randomly with respect to each other in interphase human cells |
title_sort | chromosomes are predominantly located randomly with respect to each other in interphase human cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173058/ https://www.ncbi.nlm.nih.gov/pubmed/12403811 http://dx.doi.org/10.1083/jcb.200206009 |
work_keys_str_mv | AT cornforthmichaeln chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells AT greulichbodekarinm chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells AT loucasbradfordd chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells AT arsuagajavier chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells AT vazquezmariel chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells AT sachsrainerk chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells AT brucknermartina chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells AT mollsmichael chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells AT hahnfeldtphilip chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells AT hlatkylynn chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells AT brennerdavidj chromosomesarepredominantlylocatedrandomlywithrespecttoeachotherininterphasehumancells |