Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts

Transformation of a contact between axon and dendrite into a synapse is accompanied by accumulation of the synaptic machinery at this site, being delivered in intracellular organelles mainly of TGN origin. Here, we report that in cultured hippocampal neurons, TGN organelles are linked via spectrin t...

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Detalles Bibliográficos
Autores principales: Sytnyk, Vladimir, Leshchyns'ka, Iryna, Delling, Markus, Dityateva, Galina, Dityatev, Alexander, Schachner, Melitta
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173095/
https://www.ncbi.nlm.nih.gov/pubmed/12438412
http://dx.doi.org/10.1083/jcb.200205098
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author Sytnyk, Vladimir
Leshchyns'ka, Iryna
Delling, Markus
Dityateva, Galina
Dityatev, Alexander
Schachner, Melitta
author_facet Sytnyk, Vladimir
Leshchyns'ka, Iryna
Delling, Markus
Dityateva, Galina
Dityatev, Alexander
Schachner, Melitta
author_sort Sytnyk, Vladimir
collection PubMed
description Transformation of a contact between axon and dendrite into a synapse is accompanied by accumulation of the synaptic machinery at this site, being delivered in intracellular organelles mainly of TGN origin. Here, we report that in cultured hippocampal neurons, TGN organelles are linked via spectrin to clusters of the neural cell adhesion molecule (NCAM) in the plasma membrane. These complexes are translocated along neurites and trapped at sites of initial neurite-to-neurite contacts within several minutes after initial contact formation. The accumulation of TGN organelles at contacts with NCAM-deficient neurons is reduced when compared with wild-type cells, suggesting that NCAM mediates the anchoring of intracellular organelles in nascent synapses.
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spelling pubmed-21730952008-05-01 Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts Sytnyk, Vladimir Leshchyns'ka, Iryna Delling, Markus Dityateva, Galina Dityatev, Alexander Schachner, Melitta J Cell Biol Article Transformation of a contact between axon and dendrite into a synapse is accompanied by accumulation of the synaptic machinery at this site, being delivered in intracellular organelles mainly of TGN origin. Here, we report that in cultured hippocampal neurons, TGN organelles are linked via spectrin to clusters of the neural cell adhesion molecule (NCAM) in the plasma membrane. These complexes are translocated along neurites and trapped at sites of initial neurite-to-neurite contacts within several minutes after initial contact formation. The accumulation of TGN organelles at contacts with NCAM-deficient neurons is reduced when compared with wild-type cells, suggesting that NCAM mediates the anchoring of intracellular organelles in nascent synapses. The Rockefeller University Press 2002-11-25 /pmc/articles/PMC2173095/ /pubmed/12438412 http://dx.doi.org/10.1083/jcb.200205098 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Sytnyk, Vladimir
Leshchyns'ka, Iryna
Delling, Markus
Dityateva, Galina
Dityatev, Alexander
Schachner, Melitta
Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts
title Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts
title_full Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts
title_fullStr Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts
title_full_unstemmed Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts
title_short Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts
title_sort neural cell adhesion molecule promotes accumulation of tgn organelles at sites of neuron-to-neuron contacts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173095/
https://www.ncbi.nlm.nih.gov/pubmed/12438412
http://dx.doi.org/10.1083/jcb.200205098
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