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The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo

In all eukaryotic organisms, the physical separation of two nascent cells must be coordinated with chromosome segregation and mitotic exit. In Saccharomyces cerevisiae and Schizosaccharomyces pombe this coordination depends on a number of genes that cooperate in intricate regulatory pathways termed...

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Autores principales: Gruneberg, Ulrike, Glotzer, Michael, Gartner, Anton, Nigg, Erich A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173158/
https://www.ncbi.nlm.nih.gov/pubmed/12213836
http://dx.doi.org/10.1083/jcb.200202054
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author Gruneberg, Ulrike
Glotzer, Michael
Gartner, Anton
Nigg, Erich A.
author_facet Gruneberg, Ulrike
Glotzer, Michael
Gartner, Anton
Nigg, Erich A.
author_sort Gruneberg, Ulrike
collection PubMed
description In all eukaryotic organisms, the physical separation of two nascent cells must be coordinated with chromosome segregation and mitotic exit. In Saccharomyces cerevisiae and Schizosaccharomyces pombe this coordination depends on a number of genes that cooperate in intricate regulatory pathways termed mitotic exit network and septum initiation network, respectively. Here we have explored the function of potentially homologous genes in a metazoan organism, Caenorhabditis elegans, using RNA-mediated interference. Of all the genes tested, only depletion of CeCDC-14, the C. elegans homologue of the budding yeast dual-specificity phosphatase Cdc14p (Clp1/Flp1p in fission yeast), caused embryonic lethality. We show that CeCDC-14 is required for cytokinesis but may be dispensable for progression of the early embryonic cell cycles. In response to depletion of CeCDC-14, embryos fail to establish a central spindle, and several proteins normally found at this structure are mislocalized. CeCDC-14 itself localizes to the central spindle in anaphase and to the midbody in telophase. It colocalizes with the mitotic kinesin ZEN-4, and the two proteins depend on each other for correct localization. These findings identify the CDC14 phosphatase as an important regulator of central spindle formation and cytokinesis in a metazoan organism.
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spelling pubmed-21731582008-05-01 The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo Gruneberg, Ulrike Glotzer, Michael Gartner, Anton Nigg, Erich A. J Cell Biol Article In all eukaryotic organisms, the physical separation of two nascent cells must be coordinated with chromosome segregation and mitotic exit. In Saccharomyces cerevisiae and Schizosaccharomyces pombe this coordination depends on a number of genes that cooperate in intricate regulatory pathways termed mitotic exit network and septum initiation network, respectively. Here we have explored the function of potentially homologous genes in a metazoan organism, Caenorhabditis elegans, using RNA-mediated interference. Of all the genes tested, only depletion of CeCDC-14, the C. elegans homologue of the budding yeast dual-specificity phosphatase Cdc14p (Clp1/Flp1p in fission yeast), caused embryonic lethality. We show that CeCDC-14 is required for cytokinesis but may be dispensable for progression of the early embryonic cell cycles. In response to depletion of CeCDC-14, embryos fail to establish a central spindle, and several proteins normally found at this structure are mislocalized. CeCDC-14 itself localizes to the central spindle in anaphase and to the midbody in telophase. It colocalizes with the mitotic kinesin ZEN-4, and the two proteins depend on each other for correct localization. These findings identify the CDC14 phosphatase as an important regulator of central spindle formation and cytokinesis in a metazoan organism. The Rockefeller University Press 2002-09-02 /pmc/articles/PMC2173158/ /pubmed/12213836 http://dx.doi.org/10.1083/jcb.200202054 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Gruneberg, Ulrike
Glotzer, Michael
Gartner, Anton
Nigg, Erich A.
The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo
title The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo
title_full The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo
title_fullStr The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo
title_full_unstemmed The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo
title_short The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo
title_sort cecdc-14 phosphatase is required for cytokinesis in the caenorhabditis elegans embryo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173158/
https://www.ncbi.nlm.nih.gov/pubmed/12213836
http://dx.doi.org/10.1083/jcb.200202054
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