Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115

p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins con...

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Autores principales: Shorter, James, Beard, Matthew B., Seemann, Joachim, Dirac-Svejstrup, A. Barbara, Warren, Graham
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173270/
https://www.ncbi.nlm.nih.gov/pubmed/11927603
http://dx.doi.org/10.1083/jcb.200112127
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author Shorter, James
Beard, Matthew B.
Seemann, Joachim
Dirac-Svejstrup, A. Barbara
Warren, Graham
author_facet Shorter, James
Beard, Matthew B.
Seemann, Joachim
Dirac-Svejstrup, A. Barbara
Warren, Graham
author_sort Shorter, James
collection PubMed
description p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28–syntaxin-5 (v-/t-SNARE) complex by first linking the SNAREs to promote their direct interaction. These events are essential for NSF-catalyzed reassembly of postmitotic Golgi vesicles and tubules into mature cisternae. Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).
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spelling pubmed-21732702008-05-01 Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115 Shorter, James Beard, Matthew B. Seemann, Joachim Dirac-Svejstrup, A. Barbara Warren, Graham J Cell Biol Article p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28–syntaxin-5 (v-/t-SNARE) complex by first linking the SNAREs to promote their direct interaction. These events are essential for NSF-catalyzed reassembly of postmitotic Golgi vesicles and tubules into mature cisternae. Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs). The Rockefeller University Press 2002-04-01 /pmc/articles/PMC2173270/ /pubmed/11927603 http://dx.doi.org/10.1083/jcb.200112127 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Shorter, James
Beard, Matthew B.
Seemann, Joachim
Dirac-Svejstrup, A. Barbara
Warren, Graham
Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115
title Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115
title_full Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115
title_fullStr Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115
title_full_unstemmed Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115
title_short Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115
title_sort sequential tethering of golgins and catalysis of snarepin assembly by the vesicle-tethering protein p115
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173270/
https://www.ncbi.nlm.nih.gov/pubmed/11927603
http://dx.doi.org/10.1083/jcb.200112127
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