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The Rho-associated protein kinase p160ROCK is required for centrosome positioning

The p160–Rho-associated coiled-coil–containing protein kinase (ROCK) is identified as a new centrosomal component. Using immunofluorescence with a variety of p160ROCK antibodies, immuno EM, and depletion with RNA interference, p160ROCK is principally bound to the mother centriole (MC) and an interce...

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Autores principales: Chevrier, Véronique, Piel, Matthieu, Collomb, Nora, Saoudi, Yasmina, Frank, Ronald, Paintrand, Michel, Narumiya, Shuh, Bornens, Michel, Job, Didier
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173415/
https://www.ncbi.nlm.nih.gov/pubmed/12034773
http://dx.doi.org/10.1083/jcb.200203034
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author Chevrier, Véronique
Piel, Matthieu
Collomb, Nora
Saoudi, Yasmina
Frank, Ronald
Paintrand, Michel
Narumiya, Shuh
Bornens, Michel
Job, Didier
author_facet Chevrier, Véronique
Piel, Matthieu
Collomb, Nora
Saoudi, Yasmina
Frank, Ronald
Paintrand, Michel
Narumiya, Shuh
Bornens, Michel
Job, Didier
author_sort Chevrier, Véronique
collection PubMed
description The p160–Rho-associated coiled-coil–containing protein kinase (ROCK) is identified as a new centrosomal component. Using immunofluorescence with a variety of p160ROCK antibodies, immuno EM, and depletion with RNA interference, p160ROCK is principally bound to the mother centriole (MC) and an intercentriolar linker. Inhibition of p160ROCK provoked centrosome splitting in G1 with the MC, which is normally positioned at the cell center and shows little motion during G1, displaying wide excursions around the cell periphery, similar to its migration toward the midbody during cytokinesis. p160ROCK inhibition late after anaphase in mitosis triggered MC migration to the midbody followed by completion of cell division. Thus, p160ROCK is required for centrosome positioning and centrosome-dependent exit from mitosis.
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spelling pubmed-21734152008-05-01 The Rho-associated protein kinase p160ROCK is required for centrosome positioning Chevrier, Véronique Piel, Matthieu Collomb, Nora Saoudi, Yasmina Frank, Ronald Paintrand, Michel Narumiya, Shuh Bornens, Michel Job, Didier J Cell Biol Article The p160–Rho-associated coiled-coil–containing protein kinase (ROCK) is identified as a new centrosomal component. Using immunofluorescence with a variety of p160ROCK antibodies, immuno EM, and depletion with RNA interference, p160ROCK is principally bound to the mother centriole (MC) and an intercentriolar linker. Inhibition of p160ROCK provoked centrosome splitting in G1 with the MC, which is normally positioned at the cell center and shows little motion during G1, displaying wide excursions around the cell periphery, similar to its migration toward the midbody during cytokinesis. p160ROCK inhibition late after anaphase in mitosis triggered MC migration to the midbody followed by completion of cell division. Thus, p160ROCK is required for centrosome positioning and centrosome-dependent exit from mitosis. The Rockefeller University Press 2002-05-28 /pmc/articles/PMC2173415/ /pubmed/12034773 http://dx.doi.org/10.1083/jcb.200203034 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Chevrier, Véronique
Piel, Matthieu
Collomb, Nora
Saoudi, Yasmina
Frank, Ronald
Paintrand, Michel
Narumiya, Shuh
Bornens, Michel
Job, Didier
The Rho-associated protein kinase p160ROCK is required for centrosome positioning
title The Rho-associated protein kinase p160ROCK is required for centrosome positioning
title_full The Rho-associated protein kinase p160ROCK is required for centrosome positioning
title_fullStr The Rho-associated protein kinase p160ROCK is required for centrosome positioning
title_full_unstemmed The Rho-associated protein kinase p160ROCK is required for centrosome positioning
title_short The Rho-associated protein kinase p160ROCK is required for centrosome positioning
title_sort rho-associated protein kinase p160rock is required for centrosome positioning
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173415/
https://www.ncbi.nlm.nih.gov/pubmed/12034773
http://dx.doi.org/10.1083/jcb.200203034
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