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Induction of secretory pathway components in yeast is associated with increased stability of their mRNA

The overexpression of certain membrane proteins is accompanied by a striking proliferation of intracellular membranes. One of the best characterized inducers of membrane proliferation is the 180-kD mammalian ribosome receptor (p180), whose expression in yeast results in increases in levels of mRNAs...

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Detalles Bibliográficos
Autores principales: Hyde, Maureen, Block-Alper, Laura, Felix, Jahaira, Webster, Paul, Meyer, David I.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173461/
https://www.ncbi.nlm.nih.gov/pubmed/11901166
http://dx.doi.org/10.1083/jcb.200112008
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author Hyde, Maureen
Block-Alper, Laura
Felix, Jahaira
Webster, Paul
Meyer, David I.
author_facet Hyde, Maureen
Block-Alper, Laura
Felix, Jahaira
Webster, Paul
Meyer, David I.
author_sort Hyde, Maureen
collection PubMed
description The overexpression of certain membrane proteins is accompanied by a striking proliferation of intracellular membranes. One of the best characterized inducers of membrane proliferation is the 180-kD mammalian ribosome receptor (p180), whose expression in yeast results in increases in levels of mRNAs encoding proteins that function in the secretory pathway, and an elevation in the cell's ability to secrete proteins. In this study we demonstrate that neither the unfolded protein response nor increased transcription accounts for membrane proliferation or the observed increase in secretory pathway mRNAs. Rather, p180-induced up-regulation of certain secretory pathway transcripts is due to a p180-mediated increase in the longevity of these mRNA species, as determined by measurements of transcriptional activity and specific mRNA turnover. Moreover, we show that the longevity of mRNA in general is substantially promoted through the process of its targeting to the membrane of the endoplasmic reticulum. With respect to the terminal differentiation of secretory tissues, results from this model system provide insights into how the expression of a single protein, p180, could result in substantial morphological and functional changes.
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spelling pubmed-21734612008-05-01 Induction of secretory pathway components in yeast is associated with increased stability of their mRNA Hyde, Maureen Block-Alper, Laura Felix, Jahaira Webster, Paul Meyer, David I. J Cell Biol Article The overexpression of certain membrane proteins is accompanied by a striking proliferation of intracellular membranes. One of the best characterized inducers of membrane proliferation is the 180-kD mammalian ribosome receptor (p180), whose expression in yeast results in increases in levels of mRNAs encoding proteins that function in the secretory pathway, and an elevation in the cell's ability to secrete proteins. In this study we demonstrate that neither the unfolded protein response nor increased transcription accounts for membrane proliferation or the observed increase in secretory pathway mRNAs. Rather, p180-induced up-regulation of certain secretory pathway transcripts is due to a p180-mediated increase in the longevity of these mRNA species, as determined by measurements of transcriptional activity and specific mRNA turnover. Moreover, we show that the longevity of mRNA in general is substantially promoted through the process of its targeting to the membrane of the endoplasmic reticulum. With respect to the terminal differentiation of secretory tissues, results from this model system provide insights into how the expression of a single protein, p180, could result in substantial morphological and functional changes. The Rockefeller University Press 2002-03-18 /pmc/articles/PMC2173461/ /pubmed/11901166 http://dx.doi.org/10.1083/jcb.200112008 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hyde, Maureen
Block-Alper, Laura
Felix, Jahaira
Webster, Paul
Meyer, David I.
Induction of secretory pathway components in yeast is associated with increased stability of their mRNA
title Induction of secretory pathway components in yeast is associated with increased stability of their mRNA
title_full Induction of secretory pathway components in yeast is associated with increased stability of their mRNA
title_fullStr Induction of secretory pathway components in yeast is associated with increased stability of their mRNA
title_full_unstemmed Induction of secretory pathway components in yeast is associated with increased stability of their mRNA
title_short Induction of secretory pathway components in yeast is associated with increased stability of their mRNA
title_sort induction of secretory pathway components in yeast is associated with increased stability of their mrna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173461/
https://www.ncbi.nlm.nih.gov/pubmed/11901166
http://dx.doi.org/10.1083/jcb.200112008
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