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The role of cytochrome c in caspase activation in Drosophila melanogaster cells
The release of cytochrome c from mitochondria is necessary for the formation of the Apaf-1 apoptosome and subsequent activation of caspase-9 in mammalian cells. However, the role of cytochrome c in caspase activation in Drosophila cells is not well understood. We demonstrate here that cytochrome c r...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173478/ https://www.ncbi.nlm.nih.gov/pubmed/11901173 http://dx.doi.org/10.1083/jcb.200111107 |
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author | Dorstyn, Loretta Read, Stuart Cakouros, Dimitrios Huh, Jun R. Hay, Bruce A. Kumar, Sharad |
author_facet | Dorstyn, Loretta Read, Stuart Cakouros, Dimitrios Huh, Jun R. Hay, Bruce A. Kumar, Sharad |
author_sort | Dorstyn, Loretta |
collection | PubMed |
description | The release of cytochrome c from mitochondria is necessary for the formation of the Apaf-1 apoptosome and subsequent activation of caspase-9 in mammalian cells. However, the role of cytochrome c in caspase activation in Drosophila cells is not well understood. We demonstrate here that cytochrome c remains associated with mitochondria during apoptosis of Drosophila cells and that the initiator caspase DRONC and effector caspase DRICE are activated after various death stimuli without any significant release of cytochrome c in the cytosol. Ectopic expression of the proapoptotic Bcl-2 protein, DEBCL, also fails to show any cytochrome c release from mitochondria. A significant proportion of cellular DRONC and DRICE appears to localize near mitochondria, suggesting that an apoptosome may form in the vicinity of mitochondria in the absence of cytochrome c release. In vitro, DRONC was recruited to a >700-kD complex, similar to the mammalian apoptosome in cell extracts supplemented with cytochrome c and dATP. These results suggest that caspase activation in insects follows a more primitive mechanism that may be the precursor to the caspase activation pathways in mammals. |
format | Text |
id | pubmed-2173478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21734782008-05-01 The role of cytochrome c in caspase activation in Drosophila melanogaster cells Dorstyn, Loretta Read, Stuart Cakouros, Dimitrios Huh, Jun R. Hay, Bruce A. Kumar, Sharad J Cell Biol Article The release of cytochrome c from mitochondria is necessary for the formation of the Apaf-1 apoptosome and subsequent activation of caspase-9 in mammalian cells. However, the role of cytochrome c in caspase activation in Drosophila cells is not well understood. We demonstrate here that cytochrome c remains associated with mitochondria during apoptosis of Drosophila cells and that the initiator caspase DRONC and effector caspase DRICE are activated after various death stimuli without any significant release of cytochrome c in the cytosol. Ectopic expression of the proapoptotic Bcl-2 protein, DEBCL, also fails to show any cytochrome c release from mitochondria. A significant proportion of cellular DRONC and DRICE appears to localize near mitochondria, suggesting that an apoptosome may form in the vicinity of mitochondria in the absence of cytochrome c release. In vitro, DRONC was recruited to a >700-kD complex, similar to the mammalian apoptosome in cell extracts supplemented with cytochrome c and dATP. These results suggest that caspase activation in insects follows a more primitive mechanism that may be the precursor to the caspase activation pathways in mammals. The Rockefeller University Press 2002-03-18 /pmc/articles/PMC2173478/ /pubmed/11901173 http://dx.doi.org/10.1083/jcb.200111107 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Dorstyn, Loretta Read, Stuart Cakouros, Dimitrios Huh, Jun R. Hay, Bruce A. Kumar, Sharad The role of cytochrome c in caspase activation in Drosophila melanogaster cells |
title | The role of cytochrome c in caspase activation in Drosophila melanogaster cells |
title_full | The role of cytochrome c in caspase activation in Drosophila melanogaster cells |
title_fullStr | The role of cytochrome c in caspase activation in Drosophila melanogaster cells |
title_full_unstemmed | The role of cytochrome c in caspase activation in Drosophila melanogaster cells |
title_short | The role of cytochrome c in caspase activation in Drosophila melanogaster cells |
title_sort | role of cytochrome c in caspase activation in drosophila melanogaster cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173478/ https://www.ncbi.nlm.nih.gov/pubmed/11901173 http://dx.doi.org/10.1083/jcb.200111107 |
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