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Dominant regulation of interendothelial cell gap formation by calcium-inhibited type 6 adenylyl cyclase
Acute transitions in cytosolic calcium ([Ca(2+)]i) through store-operated calcium entry channels catalyze interendothelial cell gap formation that increases permeability. However, the rise in [Ca(2+)]i only disrupts barrier function in the absence of a rise in cAMP. Discovery that type 6 adenylyl cy...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173565/ https://www.ncbi.nlm.nih.gov/pubmed/12082084 http://dx.doi.org/10.1083/jcb.200204022 |
Sumario: | Acute transitions in cytosolic calcium ([Ca(2+)]i) through store-operated calcium entry channels catalyze interendothelial cell gap formation that increases permeability. However, the rise in [Ca(2+)]i only disrupts barrier function in the absence of a rise in cAMP. Discovery that type 6 adenylyl cyclase (AC(6); EC 4.6.6.1) is inhibited by calcium entry through store-operated calcium entry pathways provided a plausible explanation for how inflammatory [Ca(2+)]i mediators may decrease cAMP necessary for endothelial cell gap formation. [Ca(2+)]i mediators only modestly decrease global cAMP concentrations and thus, to date, the physiological role of AC(6) is unresolved. Present studies used an adenoviral construct that expresses the calcium-stimulated AC(8) to convert normal calcium inhibition into stimulation of cAMP, within physiologically relevant concentration ranges. Thrombin stimulated a dose-dependent [Ca(2+)]i rise in both pulmonary artery (PAECs) and microvascular (PMVEC) endothelial cells, and promoted intercellular gap formation in both cell types. In PAECs, gap formation was progressive over 2 h, whereas in PMVECs, gap formation was rapid (within 10 min) and gaps resealed within 2 h. Expression of AC(8) resulted in a modest calcium stimulation of cAMP, which virtually abolished thrombin-induced gap formation in PMVECs. Findings provide the first direct evidence that calcium inhibition of AC(6) is essential for endothelial gap formation. |
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