A J-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria

Transport of preproteins into the mitochondrial matrix is mediated by the presequence translocase–associated motor (PAM). Three essential subunits of the motor are known: mitochondrial Hsp70 (mtHsp70); the peripheral membrane protein Tim44; and the nucleotide exchange factor Mge1. We have identified...

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Autores principales: Truscott, Kaye N., Voos, Wolfgang, Frazier, Ann E., Lind, Maria, Li, Yanfeng, Geissler, Andreas, Dudek, Jan, Müller, Hanne, Sickmann, Albert, Meyer, Helmut E., Meisinger, Chris, Guiard, Bernard, Rehling, Peter, Pfanner, Nikolaus
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173675/
https://www.ncbi.nlm.nih.gov/pubmed/14638855
http://dx.doi.org/10.1083/jcb.200308004
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author Truscott, Kaye N.
Voos, Wolfgang
Frazier, Ann E.
Lind, Maria
Li, Yanfeng
Geissler, Andreas
Dudek, Jan
Müller, Hanne
Sickmann, Albert
Meyer, Helmut E.
Meisinger, Chris
Guiard, Bernard
Rehling, Peter
Pfanner, Nikolaus
author_facet Truscott, Kaye N.
Voos, Wolfgang
Frazier, Ann E.
Lind, Maria
Li, Yanfeng
Geissler, Andreas
Dudek, Jan
Müller, Hanne
Sickmann, Albert
Meyer, Helmut E.
Meisinger, Chris
Guiard, Bernard
Rehling, Peter
Pfanner, Nikolaus
author_sort Truscott, Kaye N.
collection PubMed
description Transport of preproteins into the mitochondrial matrix is mediated by the presequence translocase–associated motor (PAM). Three essential subunits of the motor are known: mitochondrial Hsp70 (mtHsp70); the peripheral membrane protein Tim44; and the nucleotide exchange factor Mge1. We have identified the fourth essential subunit of the PAM, an essential inner membrane protein of 18 kD with a J-domain that stimulates the ATPase activity of mtHsp70. The novel J-protein (encoded by PAM18/YLR008c/TIM14) is required for the interaction of mtHsp70 with Tim44 and protein translocation into the matrix. We conclude that the reaction cycle of the PAM of mitochondria involves an essential J-protein.
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spelling pubmed-21736752008-05-01 A J-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria Truscott, Kaye N. Voos, Wolfgang Frazier, Ann E. Lind, Maria Li, Yanfeng Geissler, Andreas Dudek, Jan Müller, Hanne Sickmann, Albert Meyer, Helmut E. Meisinger, Chris Guiard, Bernard Rehling, Peter Pfanner, Nikolaus J Cell Biol Report Transport of preproteins into the mitochondrial matrix is mediated by the presequence translocase–associated motor (PAM). Three essential subunits of the motor are known: mitochondrial Hsp70 (mtHsp70); the peripheral membrane protein Tim44; and the nucleotide exchange factor Mge1. We have identified the fourth essential subunit of the PAM, an essential inner membrane protein of 18 kD with a J-domain that stimulates the ATPase activity of mtHsp70. The novel J-protein (encoded by PAM18/YLR008c/TIM14) is required for the interaction of mtHsp70 with Tim44 and protein translocation into the matrix. We conclude that the reaction cycle of the PAM of mitochondria involves an essential J-protein. The Rockefeller University Press 2003-11-24 /pmc/articles/PMC2173675/ /pubmed/14638855 http://dx.doi.org/10.1083/jcb.200308004 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Report
Truscott, Kaye N.
Voos, Wolfgang
Frazier, Ann E.
Lind, Maria
Li, Yanfeng
Geissler, Andreas
Dudek, Jan
Müller, Hanne
Sickmann, Albert
Meyer, Helmut E.
Meisinger, Chris
Guiard, Bernard
Rehling, Peter
Pfanner, Nikolaus
A J-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria
title A J-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria
title_full A J-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria
title_fullStr A J-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria
title_full_unstemmed A J-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria
title_short A J-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria
title_sort j-protein is an essential subunit of the presequence translocase–associated protein import motor of mitochondria
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173675/
https://www.ncbi.nlm.nih.gov/pubmed/14638855
http://dx.doi.org/10.1083/jcb.200308004
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