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The stromal cell–derived factor-1α/CXCR4 ligand–receptor axis is critical for progenitor survival and migration in the pancreas

The SDF-1α/CXCR4 ligand/chemokine receptor pair is required for appropriate patterning during ontogeny and stimulates the growth and differentiation of critical cell types. Here, we demonstrate SDF-1α and CXCR4 expression in fetal pancreas. We have found that SDF-1α and its receptor CXCR4 are expres...

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Autores principales: Kayali, Ayse G., Van Gunst, Kurt, Campbell, Iain L., Stotland, Aleksandr, Kritzik, Marcie, Liu, Guoxun, Flodström-Tullberg, Malin, Zhang, You-Qing, Sarvetnick, Nora
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173676/
https://www.ncbi.nlm.nih.gov/pubmed/14638861
http://dx.doi.org/10.1083/jcb.200304153
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author Kayali, Ayse G.
Van Gunst, Kurt
Campbell, Iain L.
Stotland, Aleksandr
Kritzik, Marcie
Liu, Guoxun
Flodström-Tullberg, Malin
Zhang, You-Qing
Sarvetnick, Nora
author_facet Kayali, Ayse G.
Van Gunst, Kurt
Campbell, Iain L.
Stotland, Aleksandr
Kritzik, Marcie
Liu, Guoxun
Flodström-Tullberg, Malin
Zhang, You-Qing
Sarvetnick, Nora
author_sort Kayali, Ayse G.
collection PubMed
description The SDF-1α/CXCR4 ligand/chemokine receptor pair is required for appropriate patterning during ontogeny and stimulates the growth and differentiation of critical cell types. Here, we demonstrate SDF-1α and CXCR4 expression in fetal pancreas. We have found that SDF-1α and its receptor CXCR4 are expressed in islets, also CXCR4 is expressed in and around the proliferating duct epithelium of the regenerating pancreas of the interferon (IFN) γ–nonobese diabetic mouse. We show that SDF-1α stimulates the phosphorylation of Akt, mitogen-activated protein kinase, and Src in pancreatic duct cells. Furthermore, migration assays indicate a stimulatory effect of SDF-1α on ductal cell migration. Importantly, blocking the SDF-1α/CXCR4 axis in IFNγ-nonobese diabetic mice resulted in diminished proliferation and increased apoptosis in the pancreatic ductal cells. Together, these data indicate that the SDF-1α–CXCR4 ligand receptor axis is an obligatory component in the maintenance of duct cell survival, proliferation, and migration during pancreatic regeneration.
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spelling pubmed-21736762008-05-01 The stromal cell–derived factor-1α/CXCR4 ligand–receptor axis is critical for progenitor survival and migration in the pancreas Kayali, Ayse G. Van Gunst, Kurt Campbell, Iain L. Stotland, Aleksandr Kritzik, Marcie Liu, Guoxun Flodström-Tullberg, Malin Zhang, You-Qing Sarvetnick, Nora J Cell Biol Article The SDF-1α/CXCR4 ligand/chemokine receptor pair is required for appropriate patterning during ontogeny and stimulates the growth and differentiation of critical cell types. Here, we demonstrate SDF-1α and CXCR4 expression in fetal pancreas. We have found that SDF-1α and its receptor CXCR4 are expressed in islets, also CXCR4 is expressed in and around the proliferating duct epithelium of the regenerating pancreas of the interferon (IFN) γ–nonobese diabetic mouse. We show that SDF-1α stimulates the phosphorylation of Akt, mitogen-activated protein kinase, and Src in pancreatic duct cells. Furthermore, migration assays indicate a stimulatory effect of SDF-1α on ductal cell migration. Importantly, blocking the SDF-1α/CXCR4 axis in IFNγ-nonobese diabetic mice resulted in diminished proliferation and increased apoptosis in the pancreatic ductal cells. Together, these data indicate that the SDF-1α–CXCR4 ligand receptor axis is an obligatory component in the maintenance of duct cell survival, proliferation, and migration during pancreatic regeneration. The Rockefeller University Press 2003-11-24 /pmc/articles/PMC2173676/ /pubmed/14638861 http://dx.doi.org/10.1083/jcb.200304153 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kayali, Ayse G.
Van Gunst, Kurt
Campbell, Iain L.
Stotland, Aleksandr
Kritzik, Marcie
Liu, Guoxun
Flodström-Tullberg, Malin
Zhang, You-Qing
Sarvetnick, Nora
The stromal cell–derived factor-1α/CXCR4 ligand–receptor axis is critical for progenitor survival and migration in the pancreas
title The stromal cell–derived factor-1α/CXCR4 ligand–receptor axis is critical for progenitor survival and migration in the pancreas
title_full The stromal cell–derived factor-1α/CXCR4 ligand–receptor axis is critical for progenitor survival and migration in the pancreas
title_fullStr The stromal cell–derived factor-1α/CXCR4 ligand–receptor axis is critical for progenitor survival and migration in the pancreas
title_full_unstemmed The stromal cell–derived factor-1α/CXCR4 ligand–receptor axis is critical for progenitor survival and migration in the pancreas
title_short The stromal cell–derived factor-1α/CXCR4 ligand–receptor axis is critical for progenitor survival and migration in the pancreas
title_sort stromal cell–derived factor-1α/cxcr4 ligand–receptor axis is critical for progenitor survival and migration in the pancreas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173676/
https://www.ncbi.nlm.nih.gov/pubmed/14638861
http://dx.doi.org/10.1083/jcb.200304153
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