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Latent TGF-β binding protein LTBP-2 decreases fibroblast adhesion to fibronectin

We have analyzed the effects of latent TGF-β binding protein 2 (LTBP-2) and its fragments on lung fibroblast adhesion. Quantitative cell adhesion assays indicated that fibroblasts do not adhere to full-length LTBP-2. Interestingly, LTBP-2 had dominant disrupting effects on the morphology of fibrobla...

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Detalles Bibliográficos
Autores principales: Hyytiäinen, Marko, Keski-Oja, Jorma
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173701/
https://www.ncbi.nlm.nih.gov/pubmed/14691143
http://dx.doi.org/10.1083/jcb.200309105
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author Hyytiäinen, Marko
Keski-Oja, Jorma
author_facet Hyytiäinen, Marko
Keski-Oja, Jorma
author_sort Hyytiäinen, Marko
collection PubMed
description We have analyzed the effects of latent TGF-β binding protein 2 (LTBP-2) and its fragments on lung fibroblast adhesion. Quantitative cell adhesion assays indicated that fibroblasts do not adhere to full-length LTBP-2. Interestingly, LTBP-2 had dominant disrupting effects on the morphology of fibroblasts adhering to fibronectin (FN). Fibroblasts plated on LTBP-2 and FN substratum exhibited less adherent morphology and displayed clearly decreased actin stress fibers than cells plated on FN. These cells formed, instead, extensive membrane ruffles. LTBP-2 had no effects on cells adhering to collagen type I. Fibroblasts adhered weakly to the NH(2)-terminal fragment of LTBP-2. Unlike FN, this fragment did not augment actin stress fiber formation. Interestingly, the adhesion-mediating and cytoskeleton-disrupting effects were localized to the same NH(2)-terminal proline-rich region of LTBP-2. LTBP-2 and its antiadhesive fragment bound to FN in vitro, and the antiadhesive fragment associated with the extracellular matrix FN fibrils. These observations reveal a potentially important role for LTBP-2 as an antiadhesive matrix component.
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spelling pubmed-21737012008-05-01 Latent TGF-β binding protein LTBP-2 decreases fibroblast adhesion to fibronectin Hyytiäinen, Marko Keski-Oja, Jorma J Cell Biol Article We have analyzed the effects of latent TGF-β binding protein 2 (LTBP-2) and its fragments on lung fibroblast adhesion. Quantitative cell adhesion assays indicated that fibroblasts do not adhere to full-length LTBP-2. Interestingly, LTBP-2 had dominant disrupting effects on the morphology of fibroblasts adhering to fibronectin (FN). Fibroblasts plated on LTBP-2 and FN substratum exhibited less adherent morphology and displayed clearly decreased actin stress fibers than cells plated on FN. These cells formed, instead, extensive membrane ruffles. LTBP-2 had no effects on cells adhering to collagen type I. Fibroblasts adhered weakly to the NH(2)-terminal fragment of LTBP-2. Unlike FN, this fragment did not augment actin stress fiber formation. Interestingly, the adhesion-mediating and cytoskeleton-disrupting effects were localized to the same NH(2)-terminal proline-rich region of LTBP-2. LTBP-2 and its antiadhesive fragment bound to FN in vitro, and the antiadhesive fragment associated with the extracellular matrix FN fibrils. These observations reveal a potentially important role for LTBP-2 as an antiadhesive matrix component. The Rockefeller University Press 2003-12-22 /pmc/articles/PMC2173701/ /pubmed/14691143 http://dx.doi.org/10.1083/jcb.200309105 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hyytiäinen, Marko
Keski-Oja, Jorma
Latent TGF-β binding protein LTBP-2 decreases fibroblast adhesion to fibronectin
title Latent TGF-β binding protein LTBP-2 decreases fibroblast adhesion to fibronectin
title_full Latent TGF-β binding protein LTBP-2 decreases fibroblast adhesion to fibronectin
title_fullStr Latent TGF-β binding protein LTBP-2 decreases fibroblast adhesion to fibronectin
title_full_unstemmed Latent TGF-β binding protein LTBP-2 decreases fibroblast adhesion to fibronectin
title_short Latent TGF-β binding protein LTBP-2 decreases fibroblast adhesion to fibronectin
title_sort latent tgf-β binding protein ltbp-2 decreases fibroblast adhesion to fibronectin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173701/
https://www.ncbi.nlm.nih.gov/pubmed/14691143
http://dx.doi.org/10.1083/jcb.200309105
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