Cargando…

TGF-β receptor kinase inhibitor enhances growth and integrity of embryonic stem cell–derived endothelial cells

Recent findings have shown that embryonic vascular progenitor cells are capable of differentiating into mural and endothelial cells. However, the molecular mechanisms that regulate their differentiation, proliferation, and endothelial sheet formation remain to be elucidated. Here, we show that membe...

Descripción completa

Detalles Bibliográficos
Autores principales: Watabe, Tetsuro, Nishihara, Ayako, Mishima, Koichi, Yamashita, Jun, Shimizu, Kiyoshi, Miyazawa, Keiji, Nishikawa, Shin-Ichi, Miyazono, Kohei
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173713/
https://www.ncbi.nlm.nih.gov/pubmed/14676305
http://dx.doi.org/10.1083/jcb.200305147
_version_ 1782145240860196864
author Watabe, Tetsuro
Nishihara, Ayako
Mishima, Koichi
Yamashita, Jun
Shimizu, Kiyoshi
Miyazawa, Keiji
Nishikawa, Shin-Ichi
Miyazono, Kohei
author_facet Watabe, Tetsuro
Nishihara, Ayako
Mishima, Koichi
Yamashita, Jun
Shimizu, Kiyoshi
Miyazawa, Keiji
Nishikawa, Shin-Ichi
Miyazono, Kohei
author_sort Watabe, Tetsuro
collection PubMed
description Recent findings have shown that embryonic vascular progenitor cells are capable of differentiating into mural and endothelial cells. However, the molecular mechanisms that regulate their differentiation, proliferation, and endothelial sheet formation remain to be elucidated. Here, we show that members of the transforming growth factor (TGF)-β superfamily play important roles during differentiation of vascular progenitor cells derived from mouse embryonic stem cells (ESCs) and from 8.5–days postcoitum embryos. TGF-β and activin inhibited proliferation and sheet formation of endothelial cells. Interestingly, SB-431542, a synthetic molecule that inhibits the kinases of receptors for TGF-β and activin, facilitated proliferation and sheet formation of ESC-derived endothelial cells. Moreover, SB-431542 up-regulated the expression of claudin-5, an endothelial specific component of tight junctions. These results suggest that endogenous TGF-β/activin signals play important roles in regulating vascular growth and permeability.
format Text
id pubmed-2173713
institution National Center for Biotechnology Information
language English
publishDate 2003
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21737132008-05-01 TGF-β receptor kinase inhibitor enhances growth and integrity of embryonic stem cell–derived endothelial cells Watabe, Tetsuro Nishihara, Ayako Mishima, Koichi Yamashita, Jun Shimizu, Kiyoshi Miyazawa, Keiji Nishikawa, Shin-Ichi Miyazono, Kohei J Cell Biol Article Recent findings have shown that embryonic vascular progenitor cells are capable of differentiating into mural and endothelial cells. However, the molecular mechanisms that regulate their differentiation, proliferation, and endothelial sheet formation remain to be elucidated. Here, we show that members of the transforming growth factor (TGF)-β superfamily play important roles during differentiation of vascular progenitor cells derived from mouse embryonic stem cells (ESCs) and from 8.5–days postcoitum embryos. TGF-β and activin inhibited proliferation and sheet formation of endothelial cells. Interestingly, SB-431542, a synthetic molecule that inhibits the kinases of receptors for TGF-β and activin, facilitated proliferation and sheet formation of ESC-derived endothelial cells. Moreover, SB-431542 up-regulated the expression of claudin-5, an endothelial specific component of tight junctions. These results suggest that endogenous TGF-β/activin signals play important roles in regulating vascular growth and permeability. The Rockefeller University Press 2003-12-22 /pmc/articles/PMC2173713/ /pubmed/14676305 http://dx.doi.org/10.1083/jcb.200305147 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Watabe, Tetsuro
Nishihara, Ayako
Mishima, Koichi
Yamashita, Jun
Shimizu, Kiyoshi
Miyazawa, Keiji
Nishikawa, Shin-Ichi
Miyazono, Kohei
TGF-β receptor kinase inhibitor enhances growth and integrity of embryonic stem cell–derived endothelial cells
title TGF-β receptor kinase inhibitor enhances growth and integrity of embryonic stem cell–derived endothelial cells
title_full TGF-β receptor kinase inhibitor enhances growth and integrity of embryonic stem cell–derived endothelial cells
title_fullStr TGF-β receptor kinase inhibitor enhances growth and integrity of embryonic stem cell–derived endothelial cells
title_full_unstemmed TGF-β receptor kinase inhibitor enhances growth and integrity of embryonic stem cell–derived endothelial cells
title_short TGF-β receptor kinase inhibitor enhances growth and integrity of embryonic stem cell–derived endothelial cells
title_sort tgf-β receptor kinase inhibitor enhances growth and integrity of embryonic stem cell–derived endothelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173713/
https://www.ncbi.nlm.nih.gov/pubmed/14676305
http://dx.doi.org/10.1083/jcb.200305147
work_keys_str_mv AT watabetetsuro tgfbreceptorkinaseinhibitorenhancesgrowthandintegrityofembryonicstemcellderivedendothelialcells
AT nishiharaayako tgfbreceptorkinaseinhibitorenhancesgrowthandintegrityofembryonicstemcellderivedendothelialcells
AT mishimakoichi tgfbreceptorkinaseinhibitorenhancesgrowthandintegrityofembryonicstemcellderivedendothelialcells
AT yamashitajun tgfbreceptorkinaseinhibitorenhancesgrowthandintegrityofembryonicstemcellderivedendothelialcells
AT shimizukiyoshi tgfbreceptorkinaseinhibitorenhancesgrowthandintegrityofembryonicstemcellderivedendothelialcells
AT miyazawakeiji tgfbreceptorkinaseinhibitorenhancesgrowthandintegrityofembryonicstemcellderivedendothelialcells
AT nishikawashinichi tgfbreceptorkinaseinhibitorenhancesgrowthandintegrityofembryonicstemcellderivedendothelialcells
AT miyazonokohei tgfbreceptorkinaseinhibitorenhancesgrowthandintegrityofembryonicstemcellderivedendothelialcells