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Mathematical model of the morphogenesis checkpoint in budding yeast

The morphogenesis checkpoint in budding yeast delays progression through the cell cycle in response to stimuli that prevent bud formation. Central to the checkpoint mechanism is Swe1 kinase: normally inactive, its activation halts cell cycle progression in G2. We propose a molecular network for Swe1...

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Detalles Bibliográficos
Autores principales: Ciliberto, Andrea, Novak, Bela, Tyson, John J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173725/
https://www.ncbi.nlm.nih.gov/pubmed/14691135
http://dx.doi.org/10.1083/jcb.200306139
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author Ciliberto, Andrea
Novak, Bela
Tyson, John J.
author_facet Ciliberto, Andrea
Novak, Bela
Tyson, John J.
author_sort Ciliberto, Andrea
collection PubMed
description The morphogenesis checkpoint in budding yeast delays progression through the cell cycle in response to stimuli that prevent bud formation. Central to the checkpoint mechanism is Swe1 kinase: normally inactive, its activation halts cell cycle progression in G2. We propose a molecular network for Swe1 control, based on published observations of budding yeast and analogous control signals in fission yeast. The proposed Swe1 network is merged with a model of cyclin-dependent kinase regulation, converted into a set of differential equations and studied by numerical simulation. The simulations accurately reproduce the phenotypes of a dozen checkpoint mutants. Among other predictions, the model attributes a new role to Hsl1, a kinase known to play a role in Swe1 degradation: Hsl1 must also be indirectly responsible for potent inhibition of Swe1 activity. The model supports the idea that the morphogenesis checkpoint, like other checkpoints, raises the cell size threshold for progression from one phase of the cell cycle to the next.
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spelling pubmed-21737252008-05-01 Mathematical model of the morphogenesis checkpoint in budding yeast Ciliberto, Andrea Novak, Bela Tyson, John J. J Cell Biol Article The morphogenesis checkpoint in budding yeast delays progression through the cell cycle in response to stimuli that prevent bud formation. Central to the checkpoint mechanism is Swe1 kinase: normally inactive, its activation halts cell cycle progression in G2. We propose a molecular network for Swe1 control, based on published observations of budding yeast and analogous control signals in fission yeast. The proposed Swe1 network is merged with a model of cyclin-dependent kinase regulation, converted into a set of differential equations and studied by numerical simulation. The simulations accurately reproduce the phenotypes of a dozen checkpoint mutants. Among other predictions, the model attributes a new role to Hsl1, a kinase known to play a role in Swe1 degradation: Hsl1 must also be indirectly responsible for potent inhibition of Swe1 activity. The model supports the idea that the morphogenesis checkpoint, like other checkpoints, raises the cell size threshold for progression from one phase of the cell cycle to the next. The Rockefeller University Press 2003-12-22 /pmc/articles/PMC2173725/ /pubmed/14691135 http://dx.doi.org/10.1083/jcb.200306139 Text en Copyright © 2003, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Ciliberto, Andrea
Novak, Bela
Tyson, John J.
Mathematical model of the morphogenesis checkpoint in budding yeast
title Mathematical model of the morphogenesis checkpoint in budding yeast
title_full Mathematical model of the morphogenesis checkpoint in budding yeast
title_fullStr Mathematical model of the morphogenesis checkpoint in budding yeast
title_full_unstemmed Mathematical model of the morphogenesis checkpoint in budding yeast
title_short Mathematical model of the morphogenesis checkpoint in budding yeast
title_sort mathematical model of the morphogenesis checkpoint in budding yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173725/
https://www.ncbi.nlm.nih.gov/pubmed/14691135
http://dx.doi.org/10.1083/jcb.200306139
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