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SadA, a novel adhesion receptor in Dictyostelium

Little is known about cell–substrate adhesion and how motile and adhesive forces work together in moving cells. The ability to rapidly screen a large number of insertional mutants prompted us to perform a genetic screen in Dictyostelium to isolate adhesion-deficient mutants. The resulting substrate...

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Detalles Bibliográficos
Autores principales: Fey, Petra, Stephens, Stephen, Titus, Margaret A., Chisholm, Rex L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173991/
https://www.ncbi.nlm.nih.gov/pubmed/12499361
http://dx.doi.org/10.1083/jcb.200206067
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author Fey, Petra
Stephens, Stephen
Titus, Margaret A.
Chisholm, Rex L.
author_facet Fey, Petra
Stephens, Stephen
Titus, Margaret A.
Chisholm, Rex L.
author_sort Fey, Petra
collection PubMed
description Little is known about cell–substrate adhesion and how motile and adhesive forces work together in moving cells. The ability to rapidly screen a large number of insertional mutants prompted us to perform a genetic screen in Dictyostelium to isolate adhesion-deficient mutants. The resulting substrate adhesion–deficient (sad) mutants grew in plastic dishes without attaching to the substrate. The cells were often larger than their wild-type parents and displayed a rough surface with many apparent blebs. One of these mutants, sadA(−), completely lacked substrate adhesion in growth medium. The sadA(−) mutant also showed slightly impaired cytokinesis, an aberrant F-actin organization, and a phagocytosis defect. Deletion of the sadA gene by homologous recombination recreated the original mutant phenotype. Expression of sadA–GFP in sadA-null cells restored the wild-type phenotype. In sadA–GFP-rescued mutant cells, sadA–GFP localized to the cell surface, appropriate for an adhesion molecule. SadA contains nine putative transmembrane domains and three conserved EGF-like repeats in a predicted extracellular domain. The EGF repeats are similar to corresponding regions in proteins known to be involved in adhesion, such as tenascins and integrins. Our data combined suggest that sadA is the first substrate adhesion receptor to be identified in Dictyostelium.
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spelling pubmed-21739912008-05-01 SadA, a novel adhesion receptor in Dictyostelium Fey, Petra Stephens, Stephen Titus, Margaret A. Chisholm, Rex L. J Cell Biol Article Little is known about cell–substrate adhesion and how motile and adhesive forces work together in moving cells. The ability to rapidly screen a large number of insertional mutants prompted us to perform a genetic screen in Dictyostelium to isolate adhesion-deficient mutants. The resulting substrate adhesion–deficient (sad) mutants grew in plastic dishes without attaching to the substrate. The cells were often larger than their wild-type parents and displayed a rough surface with many apparent blebs. One of these mutants, sadA(−), completely lacked substrate adhesion in growth medium. The sadA(−) mutant also showed slightly impaired cytokinesis, an aberrant F-actin organization, and a phagocytosis defect. Deletion of the sadA gene by homologous recombination recreated the original mutant phenotype. Expression of sadA–GFP in sadA-null cells restored the wild-type phenotype. In sadA–GFP-rescued mutant cells, sadA–GFP localized to the cell surface, appropriate for an adhesion molecule. SadA contains nine putative transmembrane domains and three conserved EGF-like repeats in a predicted extracellular domain. The EGF repeats are similar to corresponding regions in proteins known to be involved in adhesion, such as tenascins and integrins. Our data combined suggest that sadA is the first substrate adhesion receptor to be identified in Dictyostelium. The Rockefeller University Press 2002-12-23 /pmc/articles/PMC2173991/ /pubmed/12499361 http://dx.doi.org/10.1083/jcb.200206067 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Fey, Petra
Stephens, Stephen
Titus, Margaret A.
Chisholm, Rex L.
SadA, a novel adhesion receptor in Dictyostelium
title SadA, a novel adhesion receptor in Dictyostelium
title_full SadA, a novel adhesion receptor in Dictyostelium
title_fullStr SadA, a novel adhesion receptor in Dictyostelium
title_full_unstemmed SadA, a novel adhesion receptor in Dictyostelium
title_short SadA, a novel adhesion receptor in Dictyostelium
title_sort sada, a novel adhesion receptor in dictyostelium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173991/
https://www.ncbi.nlm.nih.gov/pubmed/12499361
http://dx.doi.org/10.1083/jcb.200206067
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