Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis

We find that Bax, a proapoptotic member of the Bcl-2 family, translocates to discrete foci on mitochondria during the initial stages of apoptosis, which subsequently become mitochondrial scission sites. A dominant negative mutant of Drp1, Drp1(K38A), inhibits apoptotic scission of mitochondria, but...

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Autores principales: Karbowski, Mariusz, Lee, Yang-Ja, Gaume, Brigitte, Jeong, Seon-Yong, Frank, Stephan, Nechushtan, Amotz, Santel, Ansgar, Fuller, Margaret, Smith, Carolyn L., Youle, Richard J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173996/
https://www.ncbi.nlm.nih.gov/pubmed/12499352
http://dx.doi.org/10.1083/jcb.200209124
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author Karbowski, Mariusz
Lee, Yang-Ja
Gaume, Brigitte
Jeong, Seon-Yong
Frank, Stephan
Nechushtan, Amotz
Santel, Ansgar
Fuller, Margaret
Smith, Carolyn L.
Youle, Richard J.
author_facet Karbowski, Mariusz
Lee, Yang-Ja
Gaume, Brigitte
Jeong, Seon-Yong
Frank, Stephan
Nechushtan, Amotz
Santel, Ansgar
Fuller, Margaret
Smith, Carolyn L.
Youle, Richard J.
author_sort Karbowski, Mariusz
collection PubMed
description We find that Bax, a proapoptotic member of the Bcl-2 family, translocates to discrete foci on mitochondria during the initial stages of apoptosis, which subsequently become mitochondrial scission sites. A dominant negative mutant of Drp1, Drp1(K38A), inhibits apoptotic scission of mitochondria, but does not inhibit Bax translocation or coalescence into foci. However, Drp1(K38A) causes the accumulation of mitochondrial fission intermediates that are associated with clusters of Bax. Surprisingly, Drp1 and Mfn2, but not other proteins implicated in the regulation of mitochondrial morphology, colocalize with Bax in these foci. We suggest that Bax participates in apoptotic fragmentation of mitochondria.
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spelling pubmed-21739962008-05-01 Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis Karbowski, Mariusz Lee, Yang-Ja Gaume, Brigitte Jeong, Seon-Yong Frank, Stephan Nechushtan, Amotz Santel, Ansgar Fuller, Margaret Smith, Carolyn L. Youle, Richard J. J Cell Biol Report We find that Bax, a proapoptotic member of the Bcl-2 family, translocates to discrete foci on mitochondria during the initial stages of apoptosis, which subsequently become mitochondrial scission sites. A dominant negative mutant of Drp1, Drp1(K38A), inhibits apoptotic scission of mitochondria, but does not inhibit Bax translocation or coalescence into foci. However, Drp1(K38A) causes the accumulation of mitochondrial fission intermediates that are associated with clusters of Bax. Surprisingly, Drp1 and Mfn2, but not other proteins implicated in the regulation of mitochondrial morphology, colocalize with Bax in these foci. We suggest that Bax participates in apoptotic fragmentation of mitochondria. The Rockefeller University Press 2002-12-23 /pmc/articles/PMC2173996/ /pubmed/12499352 http://dx.doi.org/10.1083/jcb.200209124 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Report
Karbowski, Mariusz
Lee, Yang-Ja
Gaume, Brigitte
Jeong, Seon-Yong
Frank, Stephan
Nechushtan, Amotz
Santel, Ansgar
Fuller, Margaret
Smith, Carolyn L.
Youle, Richard J.
Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis
title Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis
title_full Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis
title_fullStr Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis
title_full_unstemmed Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis
title_short Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis
title_sort spatial and temporal association of bax with mitochondrial fission sites, drp1, and mfn2 during apoptosis
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173996/
https://www.ncbi.nlm.nih.gov/pubmed/12499352
http://dx.doi.org/10.1083/jcb.200209124
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