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Ren : a novel, developmentally regulated gene that promotes neural cell differentiation

Expansion and fate choice of pluripotent stem cells along the neuroectodermal lineage is regulated by a number of signals, including EGF, retinoic acid, and NGF, which also control the proliferation and differentiation of central nervous system (CNS) and peripheral nervous system (PNS) neural progen...

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Autores principales: Gallo, Rita, Zazzeroni, Francesca, Alesse, Edoardo, Mincione, Claudia, Borello, Ugo, Buanne, Pasquale, D'Eugenio, Roberta, Mackay, Andrew R., Argenti, Beatrice, Gradini, Roberto, Russo, Matteo A., Maroder, Marella, Cossu, Giulio, Frati, Luigi, Screpanti, Isabella, Gulino, Alberto
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174014/
https://www.ncbi.nlm.nih.gov/pubmed/12186855
http://dx.doi.org/10.1083/jcb.200202024
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author Gallo, Rita
Zazzeroni, Francesca
Alesse, Edoardo
Mincione, Claudia
Borello, Ugo
Buanne, Pasquale
D'Eugenio, Roberta
Mackay, Andrew R.
Argenti, Beatrice
Gradini, Roberto
Russo, Matteo A.
Maroder, Marella
Cossu, Giulio
Frati, Luigi
Screpanti, Isabella
Gulino, Alberto
author_facet Gallo, Rita
Zazzeroni, Francesca
Alesse, Edoardo
Mincione, Claudia
Borello, Ugo
Buanne, Pasquale
D'Eugenio, Roberta
Mackay, Andrew R.
Argenti, Beatrice
Gradini, Roberto
Russo, Matteo A.
Maroder, Marella
Cossu, Giulio
Frati, Luigi
Screpanti, Isabella
Gulino, Alberto
author_sort Gallo, Rita
collection PubMed
description Expansion and fate choice of pluripotent stem cells along the neuroectodermal lineage is regulated by a number of signals, including EGF, retinoic acid, and NGF, which also control the proliferation and differentiation of central nervous system (CNS) and peripheral nervous system (PNS) neural progenitor cells. We report here the identification of a novel gene, REN, upregulated by neurogenic signals (retinoic acid, EGF, and NGF) in pluripotent embryonal stem (ES) cells and neural progenitor cell lines in association with neurotypic differentiation. Consistent with a role in neural promotion, REN overexpression induced neuronal differentiation as well as growth arrest and p27(Kip1) expression in CNS and PNS neural progenitor cell lines, and its inhibition impaired retinoic acid induction of neurogenin-1 and NeuroD expression. REN expression is developmentally regulated, initially detected in the neural fold epithelium of the mouse embryo during gastrulation, and subsequently throughout the ventral neural tube, the outer layer of the ventricular encephalic neuroepithelium and in neural crest derivatives including dorsal root ganglia. We propose that REN represents a novel component of the neurogenic signaling cascade induced by retinoic acid, EGF, and NGF, and is both a marker and a regulator of neuronal differentiation.
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spelling pubmed-21740142008-05-01 Ren : a novel, developmentally regulated gene that promotes neural cell differentiation Gallo, Rita Zazzeroni, Francesca Alesse, Edoardo Mincione, Claudia Borello, Ugo Buanne, Pasquale D'Eugenio, Roberta Mackay, Andrew R. Argenti, Beatrice Gradini, Roberto Russo, Matteo A. Maroder, Marella Cossu, Giulio Frati, Luigi Screpanti, Isabella Gulino, Alberto J Cell Biol Article Expansion and fate choice of pluripotent stem cells along the neuroectodermal lineage is regulated by a number of signals, including EGF, retinoic acid, and NGF, which also control the proliferation and differentiation of central nervous system (CNS) and peripheral nervous system (PNS) neural progenitor cells. We report here the identification of a novel gene, REN, upregulated by neurogenic signals (retinoic acid, EGF, and NGF) in pluripotent embryonal stem (ES) cells and neural progenitor cell lines in association with neurotypic differentiation. Consistent with a role in neural promotion, REN overexpression induced neuronal differentiation as well as growth arrest and p27(Kip1) expression in CNS and PNS neural progenitor cell lines, and its inhibition impaired retinoic acid induction of neurogenin-1 and NeuroD expression. REN expression is developmentally regulated, initially detected in the neural fold epithelium of the mouse embryo during gastrulation, and subsequently throughout the ventral neural tube, the outer layer of the ventricular encephalic neuroepithelium and in neural crest derivatives including dorsal root ganglia. We propose that REN represents a novel component of the neurogenic signaling cascade induced by retinoic acid, EGF, and NGF, and is both a marker and a regulator of neuronal differentiation. The Rockefeller University Press 2002-08-19 /pmc/articles/PMC2174014/ /pubmed/12186855 http://dx.doi.org/10.1083/jcb.200202024 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Gallo, Rita
Zazzeroni, Francesca
Alesse, Edoardo
Mincione, Claudia
Borello, Ugo
Buanne, Pasquale
D'Eugenio, Roberta
Mackay, Andrew R.
Argenti, Beatrice
Gradini, Roberto
Russo, Matteo A.
Maroder, Marella
Cossu, Giulio
Frati, Luigi
Screpanti, Isabella
Gulino, Alberto
Ren : a novel, developmentally regulated gene that promotes neural cell differentiation
title Ren : a novel, developmentally regulated gene that promotes neural cell differentiation
title_full Ren : a novel, developmentally regulated gene that promotes neural cell differentiation
title_fullStr Ren : a novel, developmentally regulated gene that promotes neural cell differentiation
title_full_unstemmed Ren : a novel, developmentally regulated gene that promotes neural cell differentiation
title_short Ren : a novel, developmentally regulated gene that promotes neural cell differentiation
title_sort ren : a novel, developmentally regulated gene that promotes neural cell differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174014/
https://www.ncbi.nlm.nih.gov/pubmed/12186855
http://dx.doi.org/10.1083/jcb.200202024
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