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Remodeling of organelle-bound actin is required for yeast vacuole fusion
Actin participates in several intracellular trafficking pathways. We now find that actin, bound to the surface of purified yeast vacuoles in the absence of cytosol or cytoskeleton, regulates the last compartment mixing stage of homotypic vacuole fusion. The Cdc42p GTPase is known to be required for...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174018/ https://www.ncbi.nlm.nih.gov/pubmed/12177043 http://dx.doi.org/10.1083/jcb.200204089 |
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author | Eitzen, Gary Wang, Li Thorngren, Naomi Wickner, William |
author_facet | Eitzen, Gary Wang, Li Thorngren, Naomi Wickner, William |
author_sort | Eitzen, Gary |
collection | PubMed |
description | Actin participates in several intracellular trafficking pathways. We now find that actin, bound to the surface of purified yeast vacuoles in the absence of cytosol or cytoskeleton, regulates the last compartment mixing stage of homotypic vacuole fusion. The Cdc42p GTPase is known to be required for vacuole fusion. We now show that proteins of the Cdc42p-regulated actin remodeling cascade (Cdc42p → Cla4p → Las17p/Vrp1p → Arp2/3 complex → actin) are enriched on isolated vacuoles. Vacuole fusion is dramatically altered by perturbation of the vacuole-bound actin, either by mutation of the ACT1 gene, addition of specific actin ligands such as latrunculin B or jasplakinolide, antibody to the actin regulatory proteins Las17p (yeast Wiskott-Aldrich syndrome protein) or Arp2/3, or deletion of actin regulatory genes. On docked vacuoles, actin is enriched at the “vertex ring” membrane microdomain where fusion occurs and is required for the terminal steps leading to membrane fusion. This role for actin may extend to other trafficking systems. |
format | Text |
id | pubmed-2174018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21740182008-05-01 Remodeling of organelle-bound actin is required for yeast vacuole fusion Eitzen, Gary Wang, Li Thorngren, Naomi Wickner, William J Cell Biol Article Actin participates in several intracellular trafficking pathways. We now find that actin, bound to the surface of purified yeast vacuoles in the absence of cytosol or cytoskeleton, regulates the last compartment mixing stage of homotypic vacuole fusion. The Cdc42p GTPase is known to be required for vacuole fusion. We now show that proteins of the Cdc42p-regulated actin remodeling cascade (Cdc42p → Cla4p → Las17p/Vrp1p → Arp2/3 complex → actin) are enriched on isolated vacuoles. Vacuole fusion is dramatically altered by perturbation of the vacuole-bound actin, either by mutation of the ACT1 gene, addition of specific actin ligands such as latrunculin B or jasplakinolide, antibody to the actin regulatory proteins Las17p (yeast Wiskott-Aldrich syndrome protein) or Arp2/3, or deletion of actin regulatory genes. On docked vacuoles, actin is enriched at the “vertex ring” membrane microdomain where fusion occurs and is required for the terminal steps leading to membrane fusion. This role for actin may extend to other trafficking systems. The Rockefeller University Press 2002-08-19 /pmc/articles/PMC2174018/ /pubmed/12177043 http://dx.doi.org/10.1083/jcb.200204089 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Eitzen, Gary Wang, Li Thorngren, Naomi Wickner, William Remodeling of organelle-bound actin is required for yeast vacuole fusion |
title | Remodeling of organelle-bound actin is required for yeast vacuole fusion |
title_full | Remodeling of organelle-bound actin is required for yeast vacuole fusion |
title_fullStr | Remodeling of organelle-bound actin is required for yeast vacuole fusion |
title_full_unstemmed | Remodeling of organelle-bound actin is required for yeast vacuole fusion |
title_short | Remodeling of organelle-bound actin is required for yeast vacuole fusion |
title_sort | remodeling of organelle-bound actin is required for yeast vacuole fusion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174018/ https://www.ncbi.nlm.nih.gov/pubmed/12177043 http://dx.doi.org/10.1083/jcb.200204089 |
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