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Coordination of opposite-polarity microtubule motors
Many cargoes move bidirectionally, frequently reversing course between plus- and minus-end microtubule travel. For such cargoes, the extent and importance of interactions between the opposite-polarity motors is unknown. In this paper we test whether opposite-polarity motors on lipid droplets in Dros...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174082/ https://www.ncbi.nlm.nih.gov/pubmed/11854311 http://dx.doi.org/10.1083/jcb.200109047 |
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author | Gross, Steven P. Welte, Michael A. Block, Steven M. Wieschaus, Eric F. |
author_facet | Gross, Steven P. Welte, Michael A. Block, Steven M. Wieschaus, Eric F. |
author_sort | Gross, Steven P. |
collection | PubMed |
description | Many cargoes move bidirectionally, frequently reversing course between plus- and minus-end microtubule travel. For such cargoes, the extent and importance of interactions between the opposite-polarity motors is unknown. In this paper we test whether opposite-polarity motors on lipid droplets in Drosophila embryos are coordinated and avoid interfering with each other's activity, or whether they engage in a tug of war. To this end we impaired the minus-end transport machinery using dynein and dynactin mutations, and then investigated whether plus-end motion was improved or disrupted. We observe a surprisingly severe impairment of plus-end motion due to these alterations of minus-end motor activity. These observations are consistent with a coordination hypothesis, but cannot be easily explained with a tug of war model. Our measurements indicate that dynactin plays a crucial role in the coordination of plus- and minus-end–directed motors. Specifically, we propose that dynactin enables dynein to participate efficiently in bidirectional transport, increasing its ability to stay “on” during minus-end motion and keeping it “off” during plus-end motion. |
format | Text |
id | pubmed-2174082 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21740822008-05-01 Coordination of opposite-polarity microtubule motors Gross, Steven P. Welte, Michael A. Block, Steven M. Wieschaus, Eric F. J Cell Biol Article Many cargoes move bidirectionally, frequently reversing course between plus- and minus-end microtubule travel. For such cargoes, the extent and importance of interactions between the opposite-polarity motors is unknown. In this paper we test whether opposite-polarity motors on lipid droplets in Drosophila embryos are coordinated and avoid interfering with each other's activity, or whether they engage in a tug of war. To this end we impaired the minus-end transport machinery using dynein and dynactin mutations, and then investigated whether plus-end motion was improved or disrupted. We observe a surprisingly severe impairment of plus-end motion due to these alterations of minus-end motor activity. These observations are consistent with a coordination hypothesis, but cannot be easily explained with a tug of war model. Our measurements indicate that dynactin plays a crucial role in the coordination of plus- and minus-end–directed motors. Specifically, we propose that dynactin enables dynein to participate efficiently in bidirectional transport, increasing its ability to stay “on” during minus-end motion and keeping it “off” during plus-end motion. The Rockefeller University Press 2002-02-18 /pmc/articles/PMC2174082/ /pubmed/11854311 http://dx.doi.org/10.1083/jcb.200109047 Text en Copyright © 2002, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Gross, Steven P. Welte, Michael A. Block, Steven M. Wieschaus, Eric F. Coordination of opposite-polarity microtubule motors |
title | Coordination of opposite-polarity microtubule motors |
title_full | Coordination of opposite-polarity microtubule motors |
title_fullStr | Coordination of opposite-polarity microtubule motors |
title_full_unstemmed | Coordination of opposite-polarity microtubule motors |
title_short | Coordination of opposite-polarity microtubule motors |
title_sort | coordination of opposite-polarity microtubule motors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174082/ https://www.ncbi.nlm.nih.gov/pubmed/11854311 http://dx.doi.org/10.1083/jcb.200109047 |
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