Cargando…

Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p

Cdc24p, the GDP/GTP exchange factor for the regulator of actin cytoskeleton Cdc42p, localizes to sites of polarized growth. Here we show that Cdc24p shuttles in and out of the yeast nucleus during vegetative growth. Far1p is necessary and sufficient for nuclear accumulation of Cdc24p, suggesting tha...

Descripción completa

Detalles Bibliográficos
Autores principales: Nern, Aljoscha, Arkowitz, Robert A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174313/
https://www.ncbi.nlm.nih.gov/pubmed/10725324
_version_ 1782145318950797312
author Nern, Aljoscha
Arkowitz, Robert A.
author_facet Nern, Aljoscha
Arkowitz, Robert A.
author_sort Nern, Aljoscha
collection PubMed
description Cdc24p, the GDP/GTP exchange factor for the regulator of actin cytoskeleton Cdc42p, localizes to sites of polarized growth. Here we show that Cdc24p shuttles in and out of the yeast nucleus during vegetative growth. Far1p is necessary and sufficient for nuclear accumulation of Cdc24p, suggesting that its nuclear import occurs via an association with Far1p. Nuclear export is triggered either by entry into the cell cycle or by mating pheromone. As Far1p is degraded upon entry into the cell cycle, cell cycle–dependent export of Cdc24p occurs in the absence of Far1p, whereas during mating similar export kinetics indicate that a Cdc24p–Far1p complex is exported. Our results suggest that the nucleus serves as a store of preformed Cdc24p–Far1p complex which is required for chemotropism.
format Text
id pubmed-2174313
institution National Center for Biotechnology Information
language English
publishDate 2000
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21743132008-05-01 Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p Nern, Aljoscha Arkowitz, Robert A. J Cell Biol Brief Report Cdc24p, the GDP/GTP exchange factor for the regulator of actin cytoskeleton Cdc42p, localizes to sites of polarized growth. Here we show that Cdc24p shuttles in and out of the yeast nucleus during vegetative growth. Far1p is necessary and sufficient for nuclear accumulation of Cdc24p, suggesting that its nuclear import occurs via an association with Far1p. Nuclear export is triggered either by entry into the cell cycle or by mating pheromone. As Far1p is degraded upon entry into the cell cycle, cell cycle–dependent export of Cdc24p occurs in the absence of Far1p, whereas during mating similar export kinetics indicate that a Cdc24p–Far1p complex is exported. Our results suggest that the nucleus serves as a store of preformed Cdc24p–Far1p complex which is required for chemotropism. The Rockefeller University Press 2000-03-20 /pmc/articles/PMC2174313/ /pubmed/10725324 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Report
Nern, Aljoscha
Arkowitz, Robert A.
Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p
title Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p
title_full Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p
title_fullStr Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p
title_full_unstemmed Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p
title_short Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p
title_sort nucleocytoplasmic shuttling of the cdc42p exchange factor cdc24p
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174313/
https://www.ncbi.nlm.nih.gov/pubmed/10725324
work_keys_str_mv AT nernaljoscha nucleocytoplasmicshuttlingofthecdc42pexchangefactorcdc24p
AT arkowitzroberta nucleocytoplasmicshuttlingofthecdc42pexchangefactorcdc24p