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Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p
Cdc24p, the GDP/GTP exchange factor for the regulator of actin cytoskeleton Cdc42p, localizes to sites of polarized growth. Here we show that Cdc24p shuttles in and out of the yeast nucleus during vegetative growth. Far1p is necessary and sufficient for nuclear accumulation of Cdc24p, suggesting tha...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2000
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174313/ https://www.ncbi.nlm.nih.gov/pubmed/10725324 |
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author | Nern, Aljoscha Arkowitz, Robert A. |
author_facet | Nern, Aljoscha Arkowitz, Robert A. |
author_sort | Nern, Aljoscha |
collection | PubMed |
description | Cdc24p, the GDP/GTP exchange factor for the regulator of actin cytoskeleton Cdc42p, localizes to sites of polarized growth. Here we show that Cdc24p shuttles in and out of the yeast nucleus during vegetative growth. Far1p is necessary and sufficient for nuclear accumulation of Cdc24p, suggesting that its nuclear import occurs via an association with Far1p. Nuclear export is triggered either by entry into the cell cycle or by mating pheromone. As Far1p is degraded upon entry into the cell cycle, cell cycle–dependent export of Cdc24p occurs in the absence of Far1p, whereas during mating similar export kinetics indicate that a Cdc24p–Far1p complex is exported. Our results suggest that the nucleus serves as a store of preformed Cdc24p–Far1p complex which is required for chemotropism. |
format | Text |
id | pubmed-2174313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21743132008-05-01 Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p Nern, Aljoscha Arkowitz, Robert A. J Cell Biol Brief Report Cdc24p, the GDP/GTP exchange factor for the regulator of actin cytoskeleton Cdc42p, localizes to sites of polarized growth. Here we show that Cdc24p shuttles in and out of the yeast nucleus during vegetative growth. Far1p is necessary and sufficient for nuclear accumulation of Cdc24p, suggesting that its nuclear import occurs via an association with Far1p. Nuclear export is triggered either by entry into the cell cycle or by mating pheromone. As Far1p is degraded upon entry into the cell cycle, cell cycle–dependent export of Cdc24p occurs in the absence of Far1p, whereas during mating similar export kinetics indicate that a Cdc24p–Far1p complex is exported. Our results suggest that the nucleus serves as a store of preformed Cdc24p–Far1p complex which is required for chemotropism. The Rockefeller University Press 2000-03-20 /pmc/articles/PMC2174313/ /pubmed/10725324 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Brief Report Nern, Aljoscha Arkowitz, Robert A. Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p |
title | Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p |
title_full | Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p |
title_fullStr | Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p |
title_full_unstemmed | Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p |
title_short | Nucleocytoplasmic Shuttling of the Cdc42p Exchange Factor Cdc24p |
title_sort | nucleocytoplasmic shuttling of the cdc42p exchange factor cdc24p |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174313/ https://www.ncbi.nlm.nih.gov/pubmed/10725324 |
work_keys_str_mv | AT nernaljoscha nucleocytoplasmicshuttlingofthecdc42pexchangefactorcdc24p AT arkowitzroberta nucleocytoplasmicshuttlingofthecdc42pexchangefactorcdc24p |