Circulating Skeletal Stem Cells

We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are distinguishable from skeletal stem cells found in bone marrow (stromal stem cells, “mesenchymal stem c...

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Detalles Bibliográficos
Autores principales: Kuznetsov, Sergei A., Mankani, Mahesh H., Gronthos, Stan, Satomura, Kazuhito, Bianco, Paolo, Robey, Pamela Gehron
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2001
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174322/
https://www.ncbi.nlm.nih.gov/pubmed/11381097
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author Kuznetsov, Sergei A.
Mankani, Mahesh H.
Gronthos, Stan
Satomura, Kazuhito
Bianco, Paolo
Robey, Pamela Gehron
author_facet Kuznetsov, Sergei A.
Mankani, Mahesh H.
Gronthos, Stan
Satomura, Kazuhito
Bianco, Paolo
Robey, Pamela Gehron
author_sort Kuznetsov, Sergei A.
collection PubMed
description We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are distinguishable from skeletal stem cells found in bone marrow (stromal stem cells, “mesenchymal stem cells”). The osteogenic potential of the blood-borne cells was proven by an in vivo transplantation assay in which either polyclonal or single colony–derived strains were transplanted into the subcutis of immunocompromised mice, and the donor origin of the fully differentiated bone cells was proven using species-specific probes. This is the first definitive proof of the existence of circulating skeletal stem cells in mammals.
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spelling pubmed-21743222008-05-01 Circulating Skeletal Stem Cells Kuznetsov, Sergei A. Mankani, Mahesh H. Gronthos, Stan Satomura, Kazuhito Bianco, Paolo Robey, Pamela Gehron J Cell Biol Report We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are distinguishable from skeletal stem cells found in bone marrow (stromal stem cells, “mesenchymal stem cells”). The osteogenic potential of the blood-borne cells was proven by an in vivo transplantation assay in which either polyclonal or single colony–derived strains were transplanted into the subcutis of immunocompromised mice, and the donor origin of the fully differentiated bone cells was proven using species-specific probes. This is the first definitive proof of the existence of circulating skeletal stem cells in mammals. The Rockefeller University Press 2001-05-28 /pmc/articles/PMC2174322/ /pubmed/11381097 Text en © 2001 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Report
Kuznetsov, Sergei A.
Mankani, Mahesh H.
Gronthos, Stan
Satomura, Kazuhito
Bianco, Paolo
Robey, Pamela Gehron
Circulating Skeletal Stem Cells
title Circulating Skeletal Stem Cells
title_full Circulating Skeletal Stem Cells
title_fullStr Circulating Skeletal Stem Cells
title_full_unstemmed Circulating Skeletal Stem Cells
title_short Circulating Skeletal Stem Cells
title_sort circulating skeletal stem cells
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174322/
https://www.ncbi.nlm.nih.gov/pubmed/11381097
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AT gronthosstan circulatingskeletalstemcells
AT satomurakazuhito circulatingskeletalstemcells
AT biancopaolo circulatingskeletalstemcells
AT robeypamelagehron circulatingskeletalstemcells

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