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Inhibition of Cytokinesis by a Lipid Metabolite, Psychosine
Although a number of cellular components of cytokinesis have been identified, little is known about the detailed mechanisms underlying this process. Here, we report that the lipid metabolite psychosine (galactosylsphingosine), derived from galactosylceramide, induced formation of multinuclear cells...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2000
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174564/ https://www.ncbi.nlm.nih.gov/pubmed/10811833 |
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author | Kanazawa, Takayuki Nakamura, Sachiko Momoi, Michiko Yamaji, Toshiyuki Takematsu, Hiromu Yano, Hajime Sabe, Hisataka Yamamoto, Akitsugu Kawasaki, Toshisuke Kozutsumi, Yasunori |
author_facet | Kanazawa, Takayuki Nakamura, Sachiko Momoi, Michiko Yamaji, Toshiyuki Takematsu, Hiromu Yano, Hajime Sabe, Hisataka Yamamoto, Akitsugu Kawasaki, Toshisuke Kozutsumi, Yasunori |
author_sort | Kanazawa, Takayuki |
collection | PubMed |
description | Although a number of cellular components of cytokinesis have been identified, little is known about the detailed mechanisms underlying this process. Here, we report that the lipid metabolite psychosine (galactosylsphingosine), derived from galactosylceramide, induced formation of multinuclear cells from a variety of nonadherent and adherent cells due to inhibition of cytokinesis. When psychosine was added to the human myelomonocyte cell line U937, which was the most sensitive among the cell lines tested, cleavage furrow formed either incompletely or almost completely. However, abnormal contractile movement was detected in which the cellular contents of one of the hemispheres of the contracting cell were transferred into its counterpart. Finally, the cleavage furrow disappeared and cytokinesis was reversed. Psychosine treatment also induced giant clots of actin filaments in the cells that probably consisted of small vacuoles with filamentous structures, suggesting that psychosine affected actin reorganization. These observations could account for the formation of multinuclear globoid cells in the brains of patients with globoid cell leukodystrophy, a neurological disorder characterized by the accumulation of psychosine due to galactosylceramidase deficiency. |
format | Text |
id | pubmed-2174564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21745642008-05-01 Inhibition of Cytokinesis by a Lipid Metabolite, Psychosine Kanazawa, Takayuki Nakamura, Sachiko Momoi, Michiko Yamaji, Toshiyuki Takematsu, Hiromu Yano, Hajime Sabe, Hisataka Yamamoto, Akitsugu Kawasaki, Toshisuke Kozutsumi, Yasunori J Cell Biol Original Article Although a number of cellular components of cytokinesis have been identified, little is known about the detailed mechanisms underlying this process. Here, we report that the lipid metabolite psychosine (galactosylsphingosine), derived from galactosylceramide, induced formation of multinuclear cells from a variety of nonadherent and adherent cells due to inhibition of cytokinesis. When psychosine was added to the human myelomonocyte cell line U937, which was the most sensitive among the cell lines tested, cleavage furrow formed either incompletely or almost completely. However, abnormal contractile movement was detected in which the cellular contents of one of the hemispheres of the contracting cell were transferred into its counterpart. Finally, the cleavage furrow disappeared and cytokinesis was reversed. Psychosine treatment also induced giant clots of actin filaments in the cells that probably consisted of small vacuoles with filamentous structures, suggesting that psychosine affected actin reorganization. These observations could account for the formation of multinuclear globoid cells in the brains of patients with globoid cell leukodystrophy, a neurological disorder characterized by the accumulation of psychosine due to galactosylceramidase deficiency. The Rockefeller University Press 2000-05-15 /pmc/articles/PMC2174564/ /pubmed/10811833 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article Kanazawa, Takayuki Nakamura, Sachiko Momoi, Michiko Yamaji, Toshiyuki Takematsu, Hiromu Yano, Hajime Sabe, Hisataka Yamamoto, Akitsugu Kawasaki, Toshisuke Kozutsumi, Yasunori Inhibition of Cytokinesis by a Lipid Metabolite, Psychosine |
title | Inhibition of Cytokinesis by a Lipid Metabolite, Psychosine |
title_full | Inhibition of Cytokinesis by a Lipid Metabolite, Psychosine |
title_fullStr | Inhibition of Cytokinesis by a Lipid Metabolite, Psychosine |
title_full_unstemmed | Inhibition of Cytokinesis by a Lipid Metabolite, Psychosine |
title_short | Inhibition of Cytokinesis by a Lipid Metabolite, Psychosine |
title_sort | inhibition of cytokinesis by a lipid metabolite, psychosine |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174564/ https://www.ncbi.nlm.nih.gov/pubmed/10811833 |
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