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PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-Glycanase

It has been proposed that cytoplasmic peptide:N-glycanase (PNGase) may be involved in the proteasome-dependent quality control machinery used to degrade newly synthesized glycoproteins that do not correctly fold in the ER. However, a lack of information about the structure of the enzyme has limited...

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Autores principales: Suzuki, Tadashi, Park, Hangil, Hollingsworth, Nancy M., Sternglanz, Rolf, Lennarz, William J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174826/
https://www.ncbi.nlm.nih.gov/pubmed/10831608
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author Suzuki, Tadashi
Park, Hangil
Hollingsworth, Nancy M.
Sternglanz, Rolf
Lennarz, William J.
author_facet Suzuki, Tadashi
Park, Hangil
Hollingsworth, Nancy M.
Sternglanz, Rolf
Lennarz, William J.
author_sort Suzuki, Tadashi
collection PubMed
description It has been proposed that cytoplasmic peptide:N-glycanase (PNGase) may be involved in the proteasome-dependent quality control machinery used to degrade newly synthesized glycoproteins that do not correctly fold in the ER. However, a lack of information about the structure of the enzyme has limited our ability to obtain insight into its precise biological function. A PNGase-defective mutant (png1-1) was identified by screening a collection of mutagenized strains for the absence of PNGase activity in cell extracts. The PNG1 gene was mapped to the left arm of chromosome XVI by genetic approaches and its open reading frame was identified. PNG1 encodes a soluble protein that, when expressed in Escherichia coli, exhibited PNGase activity. PNG1 may be required for efficient proteasome-mediated degradation of a misfolded glycoprotein. Subcellular localization studies indicate that Png1p is present in the nucleus as well as the cytosol. Sequencing of expressed sequence tag clones revealed that Png1p is highly conserved in a wide variety of eukaryotes including mammals, suggesting that the enzyme has an important function.
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spelling pubmed-21748262008-05-01 PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-Glycanase Suzuki, Tadashi Park, Hangil Hollingsworth, Nancy M. Sternglanz, Rolf Lennarz, William J. J Cell Biol Original Article It has been proposed that cytoplasmic peptide:N-glycanase (PNGase) may be involved in the proteasome-dependent quality control machinery used to degrade newly synthesized glycoproteins that do not correctly fold in the ER. However, a lack of information about the structure of the enzyme has limited our ability to obtain insight into its precise biological function. A PNGase-defective mutant (png1-1) was identified by screening a collection of mutagenized strains for the absence of PNGase activity in cell extracts. The PNG1 gene was mapped to the left arm of chromosome XVI by genetic approaches and its open reading frame was identified. PNG1 encodes a soluble protein that, when expressed in Escherichia coli, exhibited PNGase activity. PNG1 may be required for efficient proteasome-mediated degradation of a misfolded glycoprotein. Subcellular localization studies indicate that Png1p is present in the nucleus as well as the cytosol. Sequencing of expressed sequence tag clones revealed that Png1p is highly conserved in a wide variety of eukaryotes including mammals, suggesting that the enzyme has an important function. The Rockefeller University Press 2000-05-29 /pmc/articles/PMC2174826/ /pubmed/10831608 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Suzuki, Tadashi
Park, Hangil
Hollingsworth, Nancy M.
Sternglanz, Rolf
Lennarz, William J.
PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-Glycanase
title PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-Glycanase
title_full PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-Glycanase
title_fullStr PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-Glycanase
title_full_unstemmed PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-Glycanase
title_short PNG1, a Yeast Gene Encoding a Highly Conserved Peptide:N-Glycanase
title_sort png1, a yeast gene encoding a highly conserved peptide:n-glycanase
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174826/
https://www.ncbi.nlm.nih.gov/pubmed/10831608
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