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Normal Fertilization Occurs with Eggs Lacking the Integrin α6β1 and Is Cd9-Dependent
Previous results, based on inhibition of fertilization by an anti–α6 integrin mAb (GoH3), suggest that the α6β1 integrin on mouse eggs functions as the receptor for sperm (Almeida, E.A., A.P. Huovila, A.E. Sutherland, L.E. Stephens, P.G. Calarco, L.M. Shaw, A.M. Mercurio, A. Sonnenberg, P. Primakoff...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2000
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175111/ https://www.ncbi.nlm.nih.gov/pubmed/10851025 |
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author | Miller, Brent J. Georges-Labouesse, Elisabeth Primakoff, Paul Myles, Diana G. |
author_facet | Miller, Brent J. Georges-Labouesse, Elisabeth Primakoff, Paul Myles, Diana G. |
author_sort | Miller, Brent J. |
collection | PubMed |
description | Previous results, based on inhibition of fertilization by an anti–α6 integrin mAb (GoH3), suggest that the α6β1 integrin on mouse eggs functions as the receptor for sperm (Almeida, E.A., A.P. Huovila, A.E. Sutherland, L.E. Stephens, P.G. Calarco, L.M. Shaw, A.M. Mercurio, A. Sonnenberg, P. Primakoff, D.G. Myles, and J.M. White. 1995. Cell. 81:1095–1104). Because the egg surface tetraspanin CD9 is essential for gamete fusion (Kaji, K., S. Oda, T. Shikano, T. Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, S. Miyazaki, and A. Kudo. 2000. Nat. Genet. 24:279–282; Le Naour, F., E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix. 2000. Science. 287:319–321; Miyado, K., G. Yamada, S. Yamada, H. Hasuwa, Y. Nakamura, F. Ryu, K. Suzuki, K. Kosai, K. Inoue, A. Ogura, M. Okabe, and E. Mekada. 2000. Science. 287:321–324) and CD9 is known to associate with integrins, recent models of gamete fusion have posited that egg CD9 acts in association with α6β1 in fusion (Chen, M.S., K.S. Tung, S.A. Coonrod, Y. Takahashi, D. Bigler, A. Chang, Y. Yamashita, P.W. Kincade, J.C. Herr, and J.M. White. 1999. Proc. Natl. Acad. Sci. USA. 96:11830–11835; Kaji, K., S. Oda, T. Shikano, T. Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, S. Miyazaki, and A. Kudo. 2000. Nat. Genet. 24:279–282; Le Naour, F., E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix. 2000. Science. 287:319–321; Miyado, K., G. Yamada, S. Yamada, H. Hasuwa, Y. Nakamura, F. Ryu, K. Su- zuki, K. Kosai, K. Inoue, A. Ogura, M. Okabe, and E. Mekada. 2000. Science. 287:321–324). Using eggs from cultured ovaries of mice lacking the α6 integrin subunit, we found that the fertilization rate, fertilization index, and sperm binding were not impaired compared with wild-type or heterozygous controls. Furthermore, a reexamination of antibody inhibition, using an assay that better simulates in vivo fertilization conditions, revealed no inhibition of fusion by the GoH3 mAb. We also found that an anti-CD9 mAb completely blocks sperm fusion with either wild-type eggs or eggs lacking α6β1. Based on these results, we conclude that the α6β1 integrin is not essential for sperm–egg fusion, and we suggest a new model in which CD9 acts by itself, or interacts with egg protein(s) other than α6β1, to function in sperm–egg fusion. |
format | Text |
id | pubmed-2175111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21751112008-05-01 Normal Fertilization Occurs with Eggs Lacking the Integrin α6β1 and Is Cd9-Dependent Miller, Brent J. Georges-Labouesse, Elisabeth Primakoff, Paul Myles, Diana G. J Cell Biol Original Article Previous results, based on inhibition of fertilization by an anti–α6 integrin mAb (GoH3), suggest that the α6β1 integrin on mouse eggs functions as the receptor for sperm (Almeida, E.A., A.P. Huovila, A.E. Sutherland, L.E. Stephens, P.G. Calarco, L.M. Shaw, A.M. Mercurio, A. Sonnenberg, P. Primakoff, D.G. Myles, and J.M. White. 1995. Cell. 81:1095–1104). Because the egg surface tetraspanin CD9 is essential for gamete fusion (Kaji, K., S. Oda, T. Shikano, T. Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, S. Miyazaki, and A. Kudo. 2000. Nat. Genet. 24:279–282; Le Naour, F., E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix. 2000. Science. 287:319–321; Miyado, K., G. Yamada, S. Yamada, H. Hasuwa, Y. Nakamura, F. Ryu, K. Suzuki, K. Kosai, K. Inoue, A. Ogura, M. Okabe, and E. Mekada. 2000. Science. 287:321–324) and CD9 is known to associate with integrins, recent models of gamete fusion have posited that egg CD9 acts in association with α6β1 in fusion (Chen, M.S., K.S. Tung, S.A. Coonrod, Y. Takahashi, D. Bigler, A. Chang, Y. Yamashita, P.W. Kincade, J.C. Herr, and J.M. White. 1999. Proc. Natl. Acad. Sci. USA. 96:11830–11835; Kaji, K., S. Oda, T. Shikano, T. Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, S. Miyazaki, and A. Kudo. 2000. Nat. Genet. 24:279–282; Le Naour, F., E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix. 2000. Science. 287:319–321; Miyado, K., G. Yamada, S. Yamada, H. Hasuwa, Y. Nakamura, F. Ryu, K. Su- zuki, K. Kosai, K. Inoue, A. Ogura, M. Okabe, and E. Mekada. 2000. Science. 287:321–324). Using eggs from cultured ovaries of mice lacking the α6 integrin subunit, we found that the fertilization rate, fertilization index, and sperm binding were not impaired compared with wild-type or heterozygous controls. Furthermore, a reexamination of antibody inhibition, using an assay that better simulates in vivo fertilization conditions, revealed no inhibition of fusion by the GoH3 mAb. We also found that an anti-CD9 mAb completely blocks sperm fusion with either wild-type eggs or eggs lacking α6β1. Based on these results, we conclude that the α6β1 integrin is not essential for sperm–egg fusion, and we suggest a new model in which CD9 acts by itself, or interacts with egg protein(s) other than α6β1, to function in sperm–egg fusion. The Rockefeller University Press 2000-06-12 /pmc/articles/PMC2175111/ /pubmed/10851025 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article Miller, Brent J. Georges-Labouesse, Elisabeth Primakoff, Paul Myles, Diana G. Normal Fertilization Occurs with Eggs Lacking the Integrin α6β1 and Is Cd9-Dependent |
title | Normal Fertilization Occurs with Eggs Lacking the Integrin α6β1 and Is Cd9-Dependent |
title_full | Normal Fertilization Occurs with Eggs Lacking the Integrin α6β1 and Is Cd9-Dependent |
title_fullStr | Normal Fertilization Occurs with Eggs Lacking the Integrin α6β1 and Is Cd9-Dependent |
title_full_unstemmed | Normal Fertilization Occurs with Eggs Lacking the Integrin α6β1 and Is Cd9-Dependent |
title_short | Normal Fertilization Occurs with Eggs Lacking the Integrin α6β1 and Is Cd9-Dependent |
title_sort | normal fertilization occurs with eggs lacking the integrin α6β1 and is cd9-dependent |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175111/ https://www.ncbi.nlm.nih.gov/pubmed/10851025 |
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