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Cyk-4: A Rho Family Gtpase Activating Protein (Gap) Required for Central Spindle Formation and Cytokinesis

During cytokinesis of animal cells, the mitotic spindle plays at least two roles. Initially, the spindle positions the contractile ring. Subsequently, the central spindle, which is composed of microtubule bundles that form during anaphase, promotes a late step in cytokinesis. How the central spindle...

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Autores principales: Jantsch-Plunger, Verena, Gönczy, Pierre, Romano, Alper, Schnabel, Heinke, Hamill, Danielle, Schnabel, Ralf, Hyman, Anthony A., Glotzer, Michael
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175131/
https://www.ncbi.nlm.nih.gov/pubmed/10871280
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author Jantsch-Plunger, Verena
Gönczy, Pierre
Romano, Alper
Schnabel, Heinke
Hamill, Danielle
Schnabel, Ralf
Hyman, Anthony A.
Glotzer, Michael
author_facet Jantsch-Plunger, Verena
Gönczy, Pierre
Romano, Alper
Schnabel, Heinke
Hamill, Danielle
Schnabel, Ralf
Hyman, Anthony A.
Glotzer, Michael
author_sort Jantsch-Plunger, Verena
collection PubMed
description During cytokinesis of animal cells, the mitotic spindle plays at least two roles. Initially, the spindle positions the contractile ring. Subsequently, the central spindle, which is composed of microtubule bundles that form during anaphase, promotes a late step in cytokinesis. How the central spindle assembles and functions in cytokinesis is poorly understood. The cyk-4 gene has been identified by genetic analysis in Caenorhabditis elegans. Embryos from cyk-4(t1689ts) mutant hermaphrodites initiate, but fail to complete, cytokinesis. These embryos also fail to assemble the central spindle. We show that the cyk-4 gene encodes a GTPase activating protein (GAP) for Rho family GTPases. CYK-4 activates GTP hydrolysis by RhoA, Rac1, and Cdc42 in vitro. RNA-mediated interference of RhoA, Rac1, and Cdc42 indicates that only RhoA is essential for cytokinesis and, thus, RhoA is the likely target of CYK-4 GAP activity for cytokinesis. CYK-4 and a CYK-4:GFP fusion protein localize to the central spindle and persist at cell division remnants. CYK-4 localization is dependent on the kinesin-like protein ZEN-4/CeMKLP1 and vice versa. These data suggest that CYK-4 and ZEN-4/CeMKLP1 cooperate in central spindle assembly. Central spindle localization of CYK-4 could accelerate GTP hydrolysis by RhoA, thereby allowing contractile ring disassembly and completion of cytokinesis.
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spelling pubmed-21751312008-05-01 Cyk-4: A Rho Family Gtpase Activating Protein (Gap) Required for Central Spindle Formation and Cytokinesis Jantsch-Plunger, Verena Gönczy, Pierre Romano, Alper Schnabel, Heinke Hamill, Danielle Schnabel, Ralf Hyman, Anthony A. Glotzer, Michael J Cell Biol Original Article During cytokinesis of animal cells, the mitotic spindle plays at least two roles. Initially, the spindle positions the contractile ring. Subsequently, the central spindle, which is composed of microtubule bundles that form during anaphase, promotes a late step in cytokinesis. How the central spindle assembles and functions in cytokinesis is poorly understood. The cyk-4 gene has been identified by genetic analysis in Caenorhabditis elegans. Embryos from cyk-4(t1689ts) mutant hermaphrodites initiate, but fail to complete, cytokinesis. These embryos also fail to assemble the central spindle. We show that the cyk-4 gene encodes a GTPase activating protein (GAP) for Rho family GTPases. CYK-4 activates GTP hydrolysis by RhoA, Rac1, and Cdc42 in vitro. RNA-mediated interference of RhoA, Rac1, and Cdc42 indicates that only RhoA is essential for cytokinesis and, thus, RhoA is the likely target of CYK-4 GAP activity for cytokinesis. CYK-4 and a CYK-4:GFP fusion protein localize to the central spindle and persist at cell division remnants. CYK-4 localization is dependent on the kinesin-like protein ZEN-4/CeMKLP1 and vice versa. These data suggest that CYK-4 and ZEN-4/CeMKLP1 cooperate in central spindle assembly. Central spindle localization of CYK-4 could accelerate GTP hydrolysis by RhoA, thereby allowing contractile ring disassembly and completion of cytokinesis. The Rockefeller University Press 2000-06-26 /pmc/articles/PMC2175131/ /pubmed/10871280 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Jantsch-Plunger, Verena
Gönczy, Pierre
Romano, Alper
Schnabel, Heinke
Hamill, Danielle
Schnabel, Ralf
Hyman, Anthony A.
Glotzer, Michael
Cyk-4: A Rho Family Gtpase Activating Protein (Gap) Required for Central Spindle Formation and Cytokinesis
title Cyk-4: A Rho Family Gtpase Activating Protein (Gap) Required for Central Spindle Formation and Cytokinesis
title_full Cyk-4: A Rho Family Gtpase Activating Protein (Gap) Required for Central Spindle Formation and Cytokinesis
title_fullStr Cyk-4: A Rho Family Gtpase Activating Protein (Gap) Required for Central Spindle Formation and Cytokinesis
title_full_unstemmed Cyk-4: A Rho Family Gtpase Activating Protein (Gap) Required for Central Spindle Formation and Cytokinesis
title_short Cyk-4: A Rho Family Gtpase Activating Protein (Gap) Required for Central Spindle Formation and Cytokinesis
title_sort cyk-4: a rho family gtpase activating protein (gap) required for central spindle formation and cytokinesis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175131/
https://www.ncbi.nlm.nih.gov/pubmed/10871280
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