A Kinesin-Related Protein, Krp(180), Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division

We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle,...

Descripción completa

Detalles Bibliográficos
Autores principales: Rogers, Gregory C., Chui, Kitty K., Lee, Edwin W., Wedaman, Karen P., Sharp, David J., Holland, Gina, Morris, Robert L., Scholey, Jonathan M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175202/
https://www.ncbi.nlm.nih.gov/pubmed/10931863
Descripción
Sumario:We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

Ejemplares similares