A Kinesin-Related Protein, Krp(180), Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division

We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle,...

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Detalles Bibliográficos
Autores principales: Rogers, Gregory C., Chui, Kitty K., Lee, Edwin W., Wedaman, Karen P., Sharp, David J., Holland, Gina, Morris, Robert L., Scholey, Jonathan M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175202/
https://www.ncbi.nlm.nih.gov/pubmed/10931863
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author Rogers, Gregory C.
Chui, Kitty K.
Lee, Edwin W.
Wedaman, Karen P.
Sharp, David J.
Holland, Gina
Morris, Robert L.
Scholey, Jonathan M.
author_facet Rogers, Gregory C.
Chui, Kitty K.
Lee, Edwin W.
Wedaman, Karen P.
Sharp, David J.
Holland, Gina
Morris, Robert L.
Scholey, Jonathan M.
author_sort Rogers, Gregory C.
collection PubMed
description We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.
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spelling pubmed-21752022008-05-01 A Kinesin-Related Protein, Krp(180), Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division Rogers, Gregory C. Chui, Kitty K. Lee, Edwin W. Wedaman, Karen P. Sharp, David J. Holland, Gina Morris, Robert L. Scholey, Jonathan M. J Cell Biol Original Article We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase. The Rockefeller University Press 2000-08-07 /pmc/articles/PMC2175202/ /pubmed/10931863 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Rogers, Gregory C.
Chui, Kitty K.
Lee, Edwin W.
Wedaman, Karen P.
Sharp, David J.
Holland, Gina
Morris, Robert L.
Scholey, Jonathan M.
A Kinesin-Related Protein, Krp(180), Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division
title A Kinesin-Related Protein, Krp(180), Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division
title_full A Kinesin-Related Protein, Krp(180), Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division
title_fullStr A Kinesin-Related Protein, Krp(180), Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division
title_full_unstemmed A Kinesin-Related Protein, Krp(180), Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division
title_short A Kinesin-Related Protein, Krp(180), Positions Prometaphase Spindle Poles during Early Sea Urchin Embryonic Cell Division
title_sort kinesin-related protein, krp(180), positions prometaphase spindle poles during early sea urchin embryonic cell division
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175202/
https://www.ncbi.nlm.nih.gov/pubmed/10931863
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